FastQCFastQC Report
Fri 10 Feb 2017
ERR1632432.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632432.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2299
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT220.9569377990430622RNA PCR Primer, Index 33 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA190.8264462809917356No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC110.4784688995215311No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA100.4349717268377556No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC90.39147455415397997No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA90.39147455415397997No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT70.3044802087864289No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT70.3044802087864289No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA70.3044802087864289No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT60.26098303610265333No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC60.26098303610265333No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT60.26098303610265333No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA60.26098303610265333No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC50.2174858634188778No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA50.2174858634188778No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50.2174858634188778No Hit
TCCTTCATCGCCTCTGACTGCCAGGGCATCCACCGTGTACGCT50.2174858634188778No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA50.2174858634188778No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA50.2174858634188778No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA50.2174858634188778No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC50.2174858634188778No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC50.2174858634188778No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT50.2174858634188778No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG50.2174858634188778No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA50.2174858634188778No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA50.2174858634188778No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT50.2174858634188778No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40.17398869073510223No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC40.17398869073510223No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC40.17398869073510223No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC40.17398869073510223No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT40.17398869073510223No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC40.17398869073510223No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC40.17398869073510223No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT40.17398869073510223No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG40.17398869073510223No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC40.17398869073510223RNA PCR Primer, Index 33 (95% over 23bp)
CATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACT40.17398869073510223No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA40.17398869073510223No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA40.17398869073510223No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC40.17398869073510223No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG40.17398869073510223No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT40.17398869073510223No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA40.17398869073510223No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA40.17398869073510223No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC40.17398869073510223No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT40.17398869073510223No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA40.17398869073510223No Hit
ACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCC40.17398869073510223No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC40.17398869073510223No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA30.13049151805132667No Hit
GTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGC30.13049151805132667No Hit
TTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCAT30.13049151805132667No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC30.13049151805132667No Hit
CTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGA30.13049151805132667No Hit
GTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCG30.13049151805132667No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA30.13049151805132667No Hit
CAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGA30.13049151805132667No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA30.13049151805132667No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG30.13049151805132667No Hit
CGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGT30.13049151805132667No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC30.13049151805132667No Hit
GCTTTGAAGTGTGGACGCCAGTCTGCATGGAGCCGACCTTGAA30.13049151805132667No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG30.13049151805132667No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG30.13049151805132667No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG30.13049151805132667No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG30.13049151805132667No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA30.13049151805132667No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA30.13049151805132667No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA30.13049151805132667No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG30.13049151805132667No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC30.13049151805132667No Hit
CCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGTTGT30.13049151805132667No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG30.13049151805132667No Hit
GATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGC30.13049151805132667No Hit
GCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGA30.13049151805132667No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA30.13049151805132667No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGCTGTC30.13049151805132667No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG30.13049151805132667No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT30.13049151805132667No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC30.13049151805132667No Hit
GGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCT30.13049151805132667No Hit
AACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCT30.13049151805132667No Hit
ATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCA30.13049151805132667No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATC30.13049151805132667No Hit
CCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGCCT30.13049151805132667No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC30.13049151805132667No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT30.13049151805132667No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC30.13049151805132667No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTA30.13049151805132667No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGAC30.13049151805132667No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC30.13049151805132667No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACC30.13049151805132667No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT30.13049151805132667No Hit
CACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTA30.13049151805132667No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA30.13049151805132667No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers