FastQCFastQC Report
Fri 10 Feb 2017
ERR1632431.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632431.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4766
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT140.2937473772555602TruSeq Adapter, Index 5 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA120.2517834662190516No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC100.20981955518254303No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA90.18883759966428873No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA70.1468736886277801No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC60.1258917331095258Illumina Paired End PCR Primer 2 (95% over 23bp)
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT60.1258917331095258No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA60.1258917331095258No Hit
GCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGG50.10490977759127151No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT50.10490977759127151No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT50.10490977759127151No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA50.10490977759127151No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC50.10490977759127151No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC50.10490977759127151No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA50.10490977759127151No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC50.10490977759127151No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG50.10490977759127151No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTC400.001663953523.12537
Produced by FastQC (version 0.11.5)