##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632431.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4766
Sequences flagged as poor quality	0
Sequence length	43
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.00041963911036	34.0	33.0	34.0	31.0	34.0
2	33.17750734368443	34.0	33.0	34.0	31.0	34.0
3	33.199118757868234	34.0	33.0	34.0	31.0	34.0
4	36.52454888795636	37.0	37.0	37.0	35.0	37.0
5	36.51070079731431	37.0	37.0	37.0	35.0	37.0
6	36.537347880822495	37.0	37.0	37.0	35.0	37.0
7	36.5180444817457	37.0	37.0	37.0	35.0	37.0
8	36.517205203524966	37.0	37.0	37.0	35.0	37.0
9	38.26982794796475	39.0	39.0	39.0	37.0	39.0
10	38.30297943768359	39.0	39.0	39.0	37.0	39.0
11	38.35774234158624	39.0	39.0	39.0	37.0	39.0
12	38.33067561896769	39.0	39.0	39.0	37.0	39.0
13	38.375577003776755	39.0	39.0	39.0	37.0	39.0
14	39.90684011749895	41.0	40.0	41.0	38.0	41.0
15	39.91019723038187	41.0	40.0	41.0	38.0	41.0
16	39.87956357532522	41.0	40.0	41.0	38.0	41.0
17	39.90453210239194	41.0	40.0	41.0	38.0	41.0
18	39.87935375577004	41.0	40.0	41.0	38.0	41.0
19	39.84347461183382	41.0	40.0	41.0	38.0	41.0
20	39.85879143936215	41.0	40.0	41.0	38.0	41.0
21	39.80633655056651	41.0	40.0	41.0	38.0	41.0
22	39.78262694083089	41.0	40.0	41.0	38.0	41.0
23	39.68254301300881	41.0	40.0	41.0	37.0	41.0
24	39.66512798992866	41.0	40.0	41.0	38.0	41.0
25	39.61728913134704	41.0	40.0	41.0	37.0	41.0
26	39.51552664708351	41.0	40.0	41.0	37.0	41.0
27	39.463071758287875	41.0	39.0	41.0	37.0	41.0
28	39.367813680234995	41.0	39.0	41.0	37.0	41.0
29	39.32417121275703	41.0	39.0	41.0	36.0	41.0
30	39.287242971044904	41.0	39.0	41.0	36.0	41.0
31	39.204993705413344	41.0	39.0	41.0	36.0	41.0
32	39.104280318925724	41.0	39.0	41.0	35.0	41.0
33	39.04888795635753	40.0	39.0	41.0	35.0	41.0
34	39.0312631137222	40.0	39.0	41.0	35.0	41.0
35	38.927822073017204	40.0	39.0	41.0	35.0	41.0
36	38.89110365086026	40.0	39.0	41.0	35.0	41.0
37	38.866974402014264	40.0	39.0	41.0	35.0	41.0
38	38.79039026437264	40.0	38.0	41.0	35.0	41.0
39	38.63638271086865	40.0	38.0	41.0	35.0	41.0
40	38.56273604699958	40.0	38.0	41.0	35.0	41.0
41	38.50167855644146	40.0	38.0	41.0	35.0	41.0
42	38.42005874947545	40.0	38.0	41.0	35.0	41.0
43	37.64015946286194	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	1.0
23	1.0
24	6.0
25	10.0
26	7.0
27	12.0
28	2.0
29	14.0
30	24.0
31	30.0
32	34.0
33	53.0
34	85.0
35	124.0
36	202.0
37	411.0
38	1019.0
39	2730.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.167016365925306	15.820394460763742	11.183382291229542	28.829206882081408
2	22.97524129248846	18.988669744020143	29.815358791439362	28.220730172052033
3	20.62526227444398	19.240453210239195	28.073856483424255	32.06042803189257
4	16.63869072597566	15.002098195551824	32.375157364666386	35.98405371380613
5	18.48510281158204	32.10239194292908	31.26311372219891	18.14939152328997
6	36.3197650020982	32.522031053294164	13.994964330675618	17.163239613932017
7	33.04657994125053	25.870751154007554	18.380193033990768	22.702475870751154
8	28.97608057070919	29.68946705832984	19.156525388166177	22.177926982794798
9	27.69618128409568	13.050776332354175	16.009232060428033	43.24381032312212
10	20.33151489718842	22.597566093159884	28.724297104490137	28.346621905161562
11	40.138480906420476	18.59001258917331	18.715904322282835	22.555602182123373
12	23.394880402853545	22.870331514897188	25.723877465379775	28.010910616869495
13	36.90725975660932	16.44985312631137	18.799832144355854	27.843054972723458
14	25.891733109525806	18.5480486781368	21.548468317247167	34.01174989509022
15	30.82249265631557	22.597566093159884	18.778850188837602	27.80109106168695
16	28.724297104490137	22.28283676038607	20.2475870751154	28.745279060008393
17	28.598405371380615	22.010071338648764	20.415442719261435	28.97608057070919
18	27.61225346202266	20.751154007553506	22.28283676038607	29.353755770037765
19	29.815358791439362	21.443558539655896	21.884179605539238	26.856903063365507
20	30.025178346621907	19.848929920268567	21.2127570289551	28.913134704154427
21	29.122954259336968	19.869911875786823	21.170793117918592	29.836340746957617
22	29.899286613512377	21.00293747377256	20.163659253042386	28.934116659672682
23	29.521611414183802	22.28283676038607	20.877045740663032	27.3185060847671
24	30.69660092320604	20.751154007553506	19.911875786823334	28.640369282417122
25	29.79437683592111	21.821233738984475	20.604280318925724	27.780109106168695
26	30.339907679395722	21.254720939991607	20.10071338648762	28.304657994125055
27	28.955098615190938	21.380612673101133	21.69534200587495	27.96894670583298
28	26.940830885438523	22.30381871590432	20.667226185480487	30.08812421317667
29	28.514477549307593	22.19890893831305	20.877045740663032	28.409567771716326
30	28.38858581619807	22.156945027276542	21.086865295845573	28.367603860679814
31	29.018044481745697	22.261854804867813	20.268569030633653	28.451531682752833
32	28.031892572387747	21.12882920688208	21.947125472093997	28.892152748636175
33	27.570289550986153	20.9819555182543	22.513638271086865	28.934116659672682
34	26.50020981955518	21.884179605539238	21.485522450692404	30.130088124213177
35	26.856903063365507	21.737305916911456	23.688627780109105	27.717163239613935
36	27.90600083927822	20.54133445237096	23.352916491817037	28.19974821653378
37	28.010910616869495	20.562316407889213	23.017205203524966	28.409567771716326
38	27.003776751993286	18.59001258917331	25.891733109525806	28.514477549307593
39	25.849769198489298	20.604280318925724	25.388166177087708	28.157784305497273
40	25.765841376416283	19.534200587494755	27.192614351657575	27.50734368443139
41	23.919429290809905	20.39446076374318	26.458245908518673	29.227864036928242
42	22.618548048678136	20.310532941670164	28.892152748636175	28.178766261015525
43	23.772555602182123	19.848929920268567	27.633235417540913	28.745279060008393
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.5
4	1.0
5	1.0
6	0.5
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	1.0
13	1.5
14	2.0
15	1.5
16	1.0
17	1.0
18	1.0
19	1.0
20	2.0
21	3.0
22	4.5
23	6.0
24	5.5
25	5.0
26	5.0
27	4.5
28	4.0
29	8.5
30	13.0
31	20.5
32	28.0
33	28.0
34	37.5
35	47.0
36	49.0
37	51.0
38	69.5
39	88.0
40	88.0
41	108.5
42	129.0
43	151.5
44	174.0
45	206.0
46	238.0
47	238.0
48	284.5
49	331.0
50	381.5
51	432.0
52	461.5
53	491.0
54	491.0
55	476.5
56	462.0
57	467.5
58	473.0
59	455.0
60	437.0
61	437.0
62	401.5
63	366.0
64	328.0
65	290.0
66	264.0
67	238.0
68	238.0
69	192.5
70	147.0
71	133.0
72	119.0
73	97.0
74	75.0
75	75.0
76	59.5
77	44.0
78	34.5
79	25.0
80	21.0
81	17.0
82	17.0
83	15.5
84	14.0
85	10.0
86	6.0
87	3.5
88	1.0
89	1.0
90	2.5
91	4.0
92	2.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4766.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.37641628199748
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.23650975889782	86.10994544691565
2	3.880597014925373	7.091900965169954
3	1.1710677382319175	3.2102391942929085
4	0.3214695752009185	1.1749895090222409
5	0.20665901262916186	0.9441879983214435
6	0.06888633754305395	0.37767519932857746
7	0.022962112514351322	0.1468736886277801
8	0.0	0.0
9	0.022962112514351322	0.18883759966428873
>10	0.06888633754305395	0.7553503986571549
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT	14	0.2937473772555602	TruSeq Adapter, Index 5 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	12	0.2517834662190516	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	10	0.20981955518254303	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	9	0.18883759966428873	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	7	0.1468736886277801	No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC	6	0.1258917331095258	Illumina Paired End PCR Primer 2 (95% over 23bp)
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	6	0.1258917331095258	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	6	0.1258917331095258	No Hit
GCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGG	5	0.10490977759127151	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	5	0.10490977759127151	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	5	0.10490977759127151	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	5	0.10490977759127151	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	5	0.10490977759127151	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	5	0.10490977759127151	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	5	0.10490977759127151	No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC	5	0.10490977759127151	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	5	0.10490977759127151	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0209819555182543	0.0
23	0.0	0.0	0.0	0.0209819555182543	0.0
24	0.0	0.0	0.0	0.0209819555182543	0.0
25	0.0	0.0	0.0	0.0209819555182543	0.0
26	0.0	0.0	0.0	0.0209819555182543	0.0
27	0.0	0.0	0.0	0.0629458665547629	0.0
28	0.0	0.0	0.0	0.1049097775912715	0.0
29	0.0	0.0	0.0	0.3357112882920688	0.0
30	0.0	0.0	0.0	0.6084767100293748	0.0
31	0.0	0.0	0.0	1.0700797314309693	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTCTC	40	0.0016639535	23.125	37
>>END_MODULE