FastQCFastQC Report
Fri 10 Feb 2017
ERR1632429.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632429.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9248
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA530.5730968858131488No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA360.38927335640138405No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT330.3568339100346021TruSeq Adapter, Index 7 (95% over 21bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA270.29195501730103807No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC250.2703287197231834No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT210.22707612456747406No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA200.21626297577854672No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT190.20544982698961936No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT180.19463667820069203No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT180.19463667820069203No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC170.1838235294117647No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC170.1838235294117647No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC170.1838235294117647No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG140.1513840830449827No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT140.1513840830449827No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG140.1513840830449827No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA130.14057093425605538No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA130.14057093425605538No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA130.14057093425605538No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC120.12975778546712802No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA120.12975778546712802No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC120.12975778546712802No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT120.12975778546712802No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC120.12975778546712802No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA110.1189446366782007No Hit
TGCGTGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACC110.1189446366782007No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA110.1189446366782007No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA110.1189446366782007No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT100.10813148788927336No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA100.10813148788927336No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC100.10813148788927336No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC100.10813148788927336No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC100.10813148788927336No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT100.10813148788927336No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT100.10813148788927336No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG100.10813148788927336No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC100.10813148788927336No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA100.10813148788927336No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTCA200.001748671437.029
CGAGCTC200.001748671437.028
GCTCACA200.001748671437.031
GCCCTAC250.005221129429.59999817
CCTACTC250.005221129429.59999819
CCACGTG250.005221129429.5999986
ACCACGT250.005221129429.5999985
CACGTGT250.005221129429.5999987
CCGCCCT250.005221129429.59999815
CCCGCCC250.005221129429.59999814
GTCCCGC250.005221129429.59999812
CGTGTCC250.005221129429.5999989
TCCCGCC250.005221129429.59999813
TCATCGA250.005221129429.59999824
CTCATCG250.005221129429.59999823
TACTCAT250.005221129429.59999821
ATCGAGC250.005221129429.59999826
CTACTCA250.005221129429.59999820
ACGTGTC250.005221129429.5999988
CCCTACT250.005221129429.59999818
Produced by FastQC (version 0.11.5)