##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632429.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9248
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.055795847750865	34.0	33.0	34.0	31.0	34.0
2	33.19312283737024	34.0	33.0	34.0	31.0	34.0
3	33.28416955017301	34.0	34.0	34.0	31.0	34.0
4	36.533412629757784	37.0	37.0	37.0	35.0	37.0
5	36.49318771626297	37.0	37.0	37.0	35.0	37.0
6	36.55471453287197	37.0	37.0	37.0	35.0	37.0
7	36.52530276816609	37.0	37.0	37.0	35.0	37.0
8	36.52951989619377	37.0	37.0	37.0	35.0	37.0
9	38.33942474048443	39.0	39.0	39.0	37.0	39.0
10	38.348399653979236	39.0	39.0	39.0	37.0	39.0
11	38.43263408304498	39.0	39.0	39.0	37.0	39.0
12	38.386245674740486	39.0	39.0	39.0	37.0	39.0
13	38.43068771626297	39.0	39.0	39.0	37.0	39.0
14	39.99351211072664	41.0	40.0	41.0	38.0	41.0
15	39.95912629757785	41.0	40.0	41.0	38.0	41.0
16	39.94517733564014	41.0	40.0	41.0	38.0	41.0
17	39.923118512110726	41.0	40.0	41.0	38.0	41.0
18	39.92614619377163	41.0	40.0	41.0	38.0	41.0
19	39.91965830449827	41.0	40.0	41.0	38.0	41.0
20	39.911764705882355	41.0	40.0	41.0	38.0	41.0
21	39.88721885813149	41.0	40.0	41.0	38.0	41.0
22	39.82796280276817	41.0	40.0	41.0	38.0	41.0
23	39.80168685121107	41.0	40.0	41.0	38.0	41.0
24	39.80384948096886	41.0	40.0	41.0	38.0	41.0
25	39.800173010380625	41.0	40.0	41.0	38.0	41.0
26	39.670739619377166	41.0	40.0	41.0	38.0	41.0
27	39.54292820069204	41.0	40.0	41.0	37.0	41.0
28	39.49178200692042	41.0	40.0	41.0	37.0	41.0
29	39.43998702422145	41.0	40.0	41.0	37.0	41.0
30	39.41965830449827	41.0	39.0	41.0	37.0	41.0
31	39.360185986159166	41.0	39.0	41.0	36.0	41.0
32	39.30190311418685	41.0	39.0	41.0	36.0	41.0
33	39.225021626297575	41.0	39.0	41.0	36.0	41.0
34	39.12954152249135	41.0	39.0	41.0	35.0	41.0
35	39.04919982698962	41.0	39.0	41.0	35.0	41.0
36	39.00032439446367	41.0	39.0	41.0	35.0	41.0
37	38.94571799307958	41.0	39.0	41.0	35.0	41.0
38	38.823205017301035	40.0	39.0	41.0	35.0	41.0
39	38.74307958477509	40.0	38.0	41.0	35.0	41.0
40	38.71355968858131	40.0	38.0	41.0	35.0	41.0
41	38.60759083044983	40.0	38.0	41.0	35.0	41.0
42	38.54498269896194	40.0	38.0	41.0	35.0	41.0
43	37.805795847750865	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	0.0
21	1.0
22	2.0
23	2.0
24	3.0
25	12.0
26	9.0
27	21.0
28	32.0
29	33.0
30	43.0
31	55.0
32	66.0
33	103.0
34	157.0
35	207.0
36	379.0
37	666.0
38	1772.0
39	5684.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.62543252595156	16.057525951557096	13.062283737024222	28.254757785467127
2	21.939878892733564	20.231401384083046	30.67690311418685	27.151816608996537
3	21.50735294117647	18.82569204152249	29.638840830449826	30.028114186851212
4	16.446799307958475	15.787197231833911	33.85596885813148	33.910034602076124
5	16.83607266435986	33.38019031141869	32.569204152249135	17.214532871972317
6	33.85596885813148	33.61807958477508	15.2681660899654	17.257785467128027
7	29.55233564013841	27.508650519031143	18.75	24.18901384083045
8	27.216695501730104	29.649653979238757	21.215397923875432	21.91825259515571
9	27.768166089965398	13.051470588235295	16.446799307958475	42.733564013840834
10	19.658304498269896	22.707612456747405	28.784602076124564	28.84948096885813
11	39.80320069204152	18.55536332179931	18.804065743944637	22.837370242214533
12	23.032006920415224	23.107698961937718	26.373269896193776	27.48702422145329
13	35.73745674740484	17.1280276816609	20.05839100346021	27.076124567474047
14	25.80017301038062	19.225778546712803	21.701989619377162	33.27205882352941
15	30.114619377162633	23.507785467128027	19.420415224913494	26.95717993079585
16	26.373269896193776	23.94031141868512	21.745242214532873	27.941176470588236
17	27.47621107266436	23.205017301038062	21.355968858131487	27.96280276816609
18	26.600346020761243	20.22058823529412	22.718425605536332	30.46064013840831
19	29.033304498269896	22.577854671280274	22.750865051903112	25.63797577854671
20	29.974048442906575	20.231401384083046	22.43728373702422	27.357266435986162
21	27.724913494809687	20.750432525951556	21.301903114186853	30.2227508650519
22	28.41695501730104	22.51297577854671	19.874567474048444	29.195501730103807
23	28.957612456747405	21.50735294117647	21.561418685121104	27.97361591695502
24	27.368079584775085	20.717993079584776	21.907439446366784	30.00648788927336
25	29.163062283737023	21.6371107266436	21.929065743944637	27.27076124567474
26	29.563148788927336	21.658737024221452	21.399221453287197	27.378892733564015
27	26.80579584775086	22.80493079584775	23.64835640138408	26.7409169550173
28	27.216695501730104	23.22664359861592	21.62629757785467	27.930363321799305
29	26.535467128027683	23.713235294117645	22.21020761245675	27.541089965397923
30	26.211072664359865	21.647923875432525	24.459342560553633	27.68166089965398
31	27.465397923875436	22.64273356401384	21.853373702422147	28.03849480968858
32	25.475778546712803	21.49653979238754	23.12932525951557	29.898356401384085
33	26.297577854671278	21.9939446366782	23.897058823529413	27.811418685121108
34	26.751730103806224	21.896626297577853	23.410467128027683	27.941176470588236
35	25.497404844290656	24.27551903114187	23.161764705882355	27.065311418685123
36	26.73010380622837	22.32915224913495	24.178200692041525	26.762543252595155
37	27.768166089965398	21.23702422145329	23.388840830449826	27.605968858131487
38	27.0977508650519	20.81531141868512	25.475778546712803	26.611159169550174
39	26.2651384083045	20.17733564013841	25.810986159169552	27.74653979238754
40	25.400086505190313	20.631487889273355	27.724913494809687	26.24351211072664
41	23.291522491349482	20.685553633217992	27.56271626297578	28.460207612456745
42	22.610294117647058	21.334342560553633	29.282006920415228	26.773356401384085
43	22.015570934256054	21.085640138408305	27.71410034602076	29.184688581314877
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.0
6	1.5
7	1.0
8	0.5
9	0.0
10	1.5
11	3.0
12	3.0
13	4.5
14	6.0
15	6.5
16	7.0
17	6.0
18	5.0
19	5.0
20	6.0
21	7.0
22	9.5
23	12.0
24	16.5
25	21.0
26	21.0
27	22.5
28	24.0
29	34.5
30	45.0
31	50.0
32	55.0
33	55.0
34	72.5
35	90.0
36	102.0
37	114.0
38	159.0
39	204.0
40	204.0
41	228.5
42	253.0
43	301.5
44	350.0
45	470.0
46	590.0
47	590.0
48	685.5
49	781.0
50	861.0
51	941.0
52	1015.5
53	1090.0
54	1090.0
55	1014.5
56	939.0
57	911.5
58	884.0
59	861.5
60	839.0
61	839.0
62	773.0
63	707.0
64	555.0
65	403.0
66	353.5
67	304.0
68	304.0
69	261.5
70	219.0
71	191.5
72	164.0
73	126.5
74	89.0
75	89.0
76	66.5
77	44.0
78	28.5
79	13.0
80	17.0
81	21.0
82	21.0
83	16.0
84	11.0
85	8.0
86	5.0
87	4.5
88	4.0
89	4.0
90	2.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	9248.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.37932525951558
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.85151886663942	71.32352941176471
2	5.748535621849885	9.126297577854672
3	1.9207192480588478	4.573961937716263
4	0.7492167279662171	2.378892733564014
5	0.46315215910638874	1.8382352941176472
6	0.27244244653316985	1.2975778546712802
7	0.2179539572265359	1.2110726643598615
8	0.19070971257321892	1.2110726643598615
9	0.06811061163329246	0.4865916955017301
>10	0.5040185260863642	5.979671280276817
>50	0.013622122326658494	0.5730968858131488
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	53	0.5730968858131488	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	36	0.38927335640138405	No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT	33	0.3568339100346021	TruSeq Adapter, Index 7 (95% over 21bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	27	0.29195501730103807	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	25	0.2703287197231834	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	21	0.22707612456747406	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	20	0.21626297577854672	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	19	0.20544982698961936	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	18	0.19463667820069203	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	18	0.19463667820069203	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	17	0.1838235294117647	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	17	0.1838235294117647	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	17	0.1838235294117647	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	14	0.1513840830449827	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	14	0.1513840830449827	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	14	0.1513840830449827	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	13	0.14057093425605538	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	13	0.14057093425605538	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	13	0.14057093425605538	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	12	0.12975778546712802	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	12	0.12975778546712802	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	12	0.12975778546712802	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	12	0.12975778546712802	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	12	0.12975778546712802	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	11	0.1189446366782007	No Hit
TGCGTGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACC	11	0.1189446366782007	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	11	0.1189446366782007	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	11	0.1189446366782007	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	10	0.10813148788927336	No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA	10	0.10813148788927336	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	10	0.10813148788927336	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	10	0.10813148788927336	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	10	0.10813148788927336	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT	10	0.10813148788927336	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	10	0.10813148788927336	No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG	10	0.10813148788927336	No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC	10	0.10813148788927336	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	10	0.10813148788927336	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.02162629757785467	0.0
28	0.0	0.0	0.0	0.10813148788927336	0.0
29	0.0	0.0	0.0	0.2703287197231834	0.0
30	0.0	0.0	0.0	0.4217128027681661	0.0
31	0.0	0.0	0.0	0.9407439446366782	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGCTCA	20	0.0017486714	37.0	29
CGAGCTC	20	0.0017486714	37.0	28
GCTCACA	20	0.0017486714	37.0	31
GCCCTAC	25	0.0052211294	29.599998	17
CCTACTC	25	0.0052211294	29.599998	19
CCACGTG	25	0.0052211294	29.599998	6
ACCACGT	25	0.0052211294	29.599998	5
CACGTGT	25	0.0052211294	29.599998	7
CCGCCCT	25	0.0052211294	29.599998	15
CCCGCCC	25	0.0052211294	29.599998	14
GTCCCGC	25	0.0052211294	29.599998	12
CGTGTCC	25	0.0052211294	29.599998	9
TCCCGCC	25	0.0052211294	29.599998	13
TCATCGA	25	0.0052211294	29.599998	24
CTCATCG	25	0.0052211294	29.599998	23
TACTCAT	25	0.0052211294	29.599998	21
ATCGAGC	25	0.0052211294	29.599998	26
CTACTCA	25	0.0052211294	29.599998	20
ACGTGTC	25	0.0052211294	29.599998	8
CCCTACT	25	0.0052211294	29.599998	18
>>END_MODULE