FastQCFastQC Report
Fri 10 Feb 2017
ERR1632427.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632427.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1033846
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA28040.2712202784553986No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA23120.2236309856593729No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA18950.183296158228595No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA16980.1642410958692107No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA16560.16017859526467193No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16240.15708335670883283No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA15300.14799109345105557No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC14480.14005954465171794No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT13890.13435269856438967No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA13710.13261162687673017No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA13670.1322247220572503No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG13490.13048365036959084No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT12820.12400299464330278No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT12500.12090775608746371No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT12400.119940494038764No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC12220.11819942235110452No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT12030.11636162445857506No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC11670.1128794810832561No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC11470.1109449569858567No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT11440.11065477837124678No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA11020.106592277766708No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC10820.10465775366930857No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA10730.10378721782547884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15750.030.1873021
TACGGCT2600.027.03846230
CTAGACA358.8687905E-426.428574
TTGTACT400.00193105623.1250024
ATACGGC3100.022.67741829
CCGACCA502.7018483E-422.1999999
GTGTAGA603.7258185E-521.5833341
CGCGGTA13500.021.37777922
GCGGTAA13250.021.36226523
GGTAATA13450.021.18215825
AACTCCG13850.020.9711195
CTTATAC11400.020.77192937
TATACAC3600.020.55555537
GTATCAA23050.020.5466382
AGCCGCG14150.020.3957619
GTCCGCT1001.2894816E-820.34999810
GGGCTAA555.142613E-420.1818181
TAGCGAA1101.7535058E-920.18181810
CCGCTTA555.142613E-420.18181825
TTTTACG555.142613E-420.1818184
Produced by FastQC (version 0.11.5)