##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632427.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1033846
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.868933090615045	34.0	31.0	34.0	31.0	34.0
2	33.02325781596098	34.0	33.0	34.0	31.0	34.0
3	33.08876273642303	34.0	33.0	34.0	31.0	34.0
4	36.449517626416316	37.0	37.0	37.0	35.0	37.0
5	36.386263524741594	37.0	37.0	37.0	35.0	37.0
6	36.42552565856037	37.0	37.0	37.0	35.0	37.0
7	36.43193086784686	37.0	37.0	37.0	35.0	37.0
8	36.39874894326621	37.0	37.0	37.0	35.0	37.0
9	38.15248305840522	39.0	38.0	39.0	37.0	39.0
10	38.16009541072848	39.0	38.0	39.0	37.0	39.0
11	38.23534936537937	39.0	39.0	39.0	37.0	39.0
12	38.19053901644926	39.0	38.0	39.0	37.0	39.0
13	38.22683842661286	39.0	39.0	39.0	37.0	39.0
14	39.70351967314281	41.0	40.0	41.0	38.0	41.0
15	39.69832257415514	41.0	40.0	41.0	38.0	41.0
16	39.6590768837912	41.0	40.0	41.0	37.0	41.0
17	39.662358803922444	41.0	40.0	41.0	37.0	41.0
18	39.661716541922104	41.0	40.0	41.0	37.0	41.0
19	39.666480307511954	41.0	40.0	41.0	38.0	41.0
20	39.641294738287904	41.0	40.0	41.0	37.0	41.0
21	39.6013526192489	41.0	40.0	41.0	37.0	41.0
22	39.570791007558185	41.0	39.0	41.0	37.0	41.0
23	39.53346629962296	41.0	39.0	41.0	37.0	41.0
24	39.50890461442033	41.0	39.0	41.0	37.0	41.0
25	39.464099101800464	41.0	39.0	41.0	37.0	41.0
26	39.37804953542404	41.0	39.0	41.0	37.0	41.0
27	39.27368292763139	40.0	39.0	41.0	36.0	41.0
28	39.23166312971178	40.0	39.0	41.0	36.0	41.0
29	39.19281304952575	40.0	39.0	41.0	36.0	41.0
30	39.147756048773225	40.0	39.0	41.0	36.0	41.0
31	39.091208942144185	40.0	39.0	41.0	35.0	41.0
32	39.03144182015503	40.0	39.0	41.0	35.0	41.0
33	38.967169191543036	40.0	39.0	41.0	35.0	41.0
34	38.92971390323124	40.0	39.0	41.0	35.0	41.0
35	38.85226716551595	40.0	38.0	41.0	35.0	41.0
36	38.80122377994402	40.0	38.0	41.0	35.0	41.0
37	38.74286015518752	40.0	38.0	41.0	35.0	41.0
38	38.65901207723394	40.0	38.0	41.0	35.0	41.0
39	38.57743609783275	40.0	38.0	41.0	35.0	41.0
40	38.50069062510277	40.0	38.0	41.0	35.0	41.0
41	38.44698049806257	40.0	38.0	41.0	35.0	41.0
42	38.37206121608054	40.0	38.0	41.0	35.0	41.0
43	37.61298007633632	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	0.0
15	3.0
16	6.0
17	8.0
18	20.0
19	35.0
20	80.0
21	160.0
22	303.0
23	482.0
24	795.0
25	1112.0
26	1584.0
27	2172.0
28	2989.0
29	4136.0
30	5536.0
31	7252.0
32	9673.0
33	12994.0
34	19574.0
35	29925.0
36	48381.0
37	94113.0
38	260461.0
39	532049.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.64010307144391	16.749593266308523	12.916817398335922	27.693486263911648
2	21.35472788016784	20.074363106304034	32.224335152430825	26.3465738610973
3	21.304817158454934	20.056662210812828	29.525383857944025	29.113136772788213
4	15.92558272702124	16.12164674429267	34.04868810248335	33.90408242620274
5	17.15632695778675	33.10493052156704	32.77625487741888	16.962487643227327
6	33.35516121356566	34.161083952542256	15.569243388280265	16.914511445611822
7	29.478568374786963	28.145971450293374	19.85653569293686	22.51892448198281
8	26.903813527353204	30.963799250565366	20.473939058621884	21.65844816345955
9	27.98269761647286	13.093245996018748	17.594883570667196	41.329172816841194
10	19.403179970711303	23.61792762171542	29.369751394308242	27.60914101326503
11	39.220541550675826	19.692391323272517	18.812569763775265	22.274497362276392
12	22.899058467121797	24.08318066713998	26.367466721349214	26.650294144389008
13	35.156590053063994	17.728752638207236	20.88870102510432	26.225956283624445
14	24.102429181909105	20.3894970817704	22.8729423918069	32.63513134451359
15	29.599089226054943	24.65608997858482	19.748589248301972	25.99623154705827
16	25.471878790458153	24.323061655217508	22.264824741789397	27.940234812534943
17	27.129475763314847	23.868448492328646	21.507071652838043	27.495004091518467
18	26.491469715992515	21.339348413593516	23.495278793940297	28.67390307647367
19	28.492734894752214	22.4960970976335	23.141841241345425	25.869326766268863
20	29.902616056936914	20.83201946905052	22.81471321647518	26.450651257537388
21	27.483300220729202	22.469497391294254	21.68514459600366	28.362057791972884
22	28.005525000822175	23.104698378675355	21.18922934363532	27.700547276867155
23	27.89622438931911	22.143916985701935	22.277495874627363	27.6823627503516
24	27.997013094793616	21.793091040638547	22.437771196096904	27.772124668470934
25	28.063850902358766	22.381669997272322	22.64776378686961	26.9067153134993
26	28.340487848286884	22.68094087514001	21.973485412721043	27.005085863852063
27	27.09513796058601	21.94630534915258	24.003768452941735	26.954788237319676
28	26.334289633078818	23.08245135155526	22.057540484753048	28.525718530612878
29	26.87576292794091	23.734192519969124	22.169839608607084	27.22020494348288
30	26.51768251751228	22.7859855336288	23.675963344637402	27.020368604221517
31	28.08377650056198	22.34955689725549	22.558195321160017	27.008471281022512
32	25.17647696078526	22.42384262259563	22.922659661110067	29.47702075550904
33	26.059877389862706	22.028329170882316	24.333798263958077	27.5779951752969
34	25.98394731903978	21.993701189538868	23.975621127324573	28.046730364096778
35	25.85046515631922	23.662131497340997	24.08037560719875	26.407027739141032
36	26.09053959680649	22.18773395650803	24.892101918467546	26.829624528217938
37	26.847131971299397	21.640263636943992	24.239200035595243	27.27340435616136
38	25.658366913447455	21.22627548010052	25.860331229215955	27.25502637723607
39	25.173091543614813	20.18347026539736	26.7456661823908	27.897772008597027
40	24.763069161171007	21.226759111124867	27.5837987475891	26.426372980115026
41	23.399519851119024	20.32130510733707	28.61963967554162	27.659535366002284
42	21.967101483199624	20.997227826968427	29.553531183561187	27.48213950627076
43	20.998678720041475	21.62111184837974	29.14234808665894	28.237861344919846
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6.0
1	6.0
2	6.0
3	19.0
4	32.0
5	32.0
6	38.0
7	44.0
8	52.0
9	60.0
10	96.5
11	133.0
12	133.0
13	223.5
14	314.0
15	631.0
16	948.0
17	982.0
18	1016.0
19	1016.0
20	1194.5
21	1373.0
22	1441.0
23	1509.0
24	1850.0
25	2191.0
26	2191.0
27	2796.0
28	3401.0
29	4939.5
30	6478.0
31	7374.5
32	8271.0
33	8271.0
34	10087.0
35	11903.0
36	13452.5
37	15002.0
38	20093.0
39	25184.0
40	25184.0
41	30161.5
42	35139.0
43	39709.0
44	44279.0
45	59422.5
46	74566.0
47	74566.0
48	84747.0
49	94928.0
50	105772.5
51	116617.0
52	121429.5
53	126242.0
54	126242.0
55	114821.5
56	103401.0
57	98986.5
58	94572.0
59	87021.5
60	79471.0
61	79471.0
62	71248.5
63	63026.0
64	51257.0
65	39488.0
66	34047.5
67	28607.0
68	28607.0
69	23935.5
70	19264.0
71	16608.5
72	13953.0
73	11013.0
74	8073.0
75	8073.0
76	6587.5
77	5102.0
78	4198.5
79	3295.0
80	2811.0
81	2327.0
82	2327.0
83	1966.5
84	1606.0
85	1286.5
86	967.0
87	768.0
88	569.0
89	569.0
90	451.5
91	334.0
92	221.5
93	109.0
94	69.0
95	29.0
96	29.0
97	18.5
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1033846.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.79479123381094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.70233836499948	33.309170810169576
2	7.9324000945859785	6.313364114942223
3	2.371643723856792	2.8313720061557697
4	1.24455544259583	1.9810729606801691
5	0.7799774718990825	1.5519520330649814
6	0.5307147449745451	1.2671809488580341
7	0.4098808881047874	1.1417787061001323
8	0.3233646875759055	1.0294584187575724
9	0.24805893909148943	0.8884308325343802
>10	1.9116906398889868	15.714053258094033
>50	0.2985190062358961	8.445687743048943
>100	0.22443669545233114	17.51458511250553
>500	0.015839723348181792	4.383373816638764
>1k	0.006579577390783206	3.6285192384498712
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2804	0.2712202784553986	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2312	0.2236309856593729	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1895	0.183296158228595	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1698	0.1642410958692107	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1656	0.16017859526467193	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1624	0.15708335670883283	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1530	0.14799109345105557	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1448	0.14005954465171794	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1389	0.13435269856438967	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1371	0.13261162687673017	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1367	0.1322247220572503	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1349	0.13048365036959084	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1282	0.12400299464330278	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	1250	0.12090775608746371	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1240	0.119940494038764	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1222	0.11819942235110452	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	1203	0.11636162445857506	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1167	0.1128794810832561	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	1147	0.1109449569858567	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1144	0.11065477837124678	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1102	0.106592277766708	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	1082	0.10465775366930857	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	1073	0.10378721782547884	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	9.672620486997096E-5	0.0
3	0.0	0.0	0.0	9.672620486997096E-5	0.0
4	0.0	0.0	0.0	9.672620486997096E-5	0.0
5	0.0	0.0	0.0	9.672620486997096E-5	0.0
6	0.0	0.0	0.0	9.672620486997096E-5	0.0
7	0.0	0.0	0.0	9.672620486997096E-5	0.0
8	9.672620486997096E-5	0.0	0.0	9.672620486997096E-5	0.0
9	9.672620486997096E-5	0.0	0.0	1.9345240973994192E-4	0.0
10	9.672620486997096E-5	0.0	0.0	1.9345240973994192E-4	0.0
11	1.9345240973994192E-4	0.0	0.0	1.9345240973994192E-4	0.0
12	1.9345240973994192E-4	0.0	0.0	1.9345240973994192E-4	0.0
13	1.9345240973994192E-4	0.0	0.0	2.9017861460991286E-4	0.0
14	1.9345240973994192E-4	0.0	0.0	3.8690481947988383E-4	0.0
15	1.9345240973994192E-4	0.0	0.0	4.836310243498548E-4	0.0
16	1.9345240973994192E-4	0.0	0.0	8.705358438297387E-4	0.0
17	1.9345240973994192E-4	0.0	0.0	0.0012574406633096226	0.0
18	2.9017861460991286E-4	0.0	0.0	0.0015476192779195353	0.0
19	2.9017861460991286E-4	0.0	0.0	0.0015476192779195353	0.0
20	3.8690481947988383E-4	0.0	0.0	0.002127976507139361	0.0
21	3.8690481947988383E-4	0.0	0.0	0.003385417170448984	0.0
22	3.8690481947988383E-4	0.0	0.0	0.005706846087328287	0.0
23	3.8690481947988383E-4	0.0	0.0	0.0069642867506379096	0.0
24	3.8690481947988383E-4	0.0	0.0	0.008511906028557444	0.0
25	3.8690481947988383E-4	0.0	0.0	0.009672620486997097	0.0
26	3.8690481947988383E-4	0.0	0.0	0.012477680428226254	0.0
27	3.8690481947988383E-4	0.0	0.0	0.032403278631440276	0.0
28	3.8690481947988383E-4	0.0	0.0	0.12313245879947303	0.0
29	3.8690481947988383E-4	0.0	0.0	0.2716071832748785	0.0
30	3.8690481947988383E-4	0.0	0.0	0.4679613791609195	0.0
31	3.8690481947988383E-4	0.0	0.0	1.005952530647698	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1575	0.0	30.187302	1
TACGGCT	260	0.0	27.038462	30
CTAGACA	35	8.8687905E-4	26.42857	4
TTGTACT	40	0.001931056	23.125002	4
ATACGGC	310	0.0	22.677418	29
CCGACCA	50	2.7018483E-4	22.199999	9
GTGTAGA	60	3.7258185E-5	21.583334	1
CGCGGTA	1350	0.0	21.377779	22
GCGGTAA	1325	0.0	21.362265	23
GGTAATA	1345	0.0	21.182158	25
AACTCCG	1385	0.0	20.971119	5
CTTATAC	1140	0.0	20.771929	37
TATACAC	360	0.0	20.555555	37
GTATCAA	2305	0.0	20.546638	2
AGCCGCG	1415	0.0	20.39576	19
GTCCGCT	100	1.2894816E-8	20.349998	10
GGGCTAA	55	5.142613E-4	20.181818	1
TAGCGAA	110	1.7535058E-9	20.181818	10
CCGCTTA	55	5.142613E-4	20.181818	25
TTTTACG	55	5.142613E-4	20.181818	4
>>END_MODULE