FastQCFastQC Report
Fri 10 Feb 2017
ERR1632426.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632426.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17676
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA500.2828694274722788No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT480.2715546503733876TruSeq Adapter, Index 1 (95% over 21bp)
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA400.22629554197782306No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA370.2093233763294863No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC340.19235121068114958No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA340.19235121068114958No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT320.18103643358225843No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA310.17537904503281285No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT290.1640642679339217No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC290.1640642679339217No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT270.15274949083503053No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA270.15274949083503053No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA270.15274949083503053No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA260.14709210228558497No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA250.1414347137361394No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG240.1357773251866938No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA230.13011993663724825No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC210.11880515953835709No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC210.11880515953835709No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA200.11314777098891153No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT190.10749038243946595No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC190.10749038243946595No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC180.10183299389002036No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA180.10183299389002036No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC180.10183299389002036No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT180.10183299389002036No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG251.18507945E-437.027
CCAGCAG358.5267803E-426.42857214
GCGGTAA358.5267803E-426.42857223
ACTCCGT358.5267803E-426.4285726
AACTCCG358.5267803E-426.4285725
TAACTCC358.5267803E-426.4285724
CTAACTC358.5267803E-426.4285723
GCTAACT358.5267803E-426.4285722
CGCGGTA358.5267803E-426.42857222
GGCTAAC358.5267803E-426.4285721
TCCGTGC358.5267803E-426.4285728
AATACGG358.5267803E-426.42857228
AGCCGCG358.5267803E-426.42857219
GTAATAC358.5267803E-426.42857226
CTCCGTG358.5267803E-426.4285727
CGGTAAT358.5267803E-426.42857224
GGTAATA358.5267803E-426.42857225
GCCAGCA400.001857282123.12513
GCAGCCG400.001857282123.12517
CGTGCCA400.001857282123.12510
Produced by FastQC (version 0.11.5)