FastQCFastQC Report
Fri 10 Feb 2017
ERR1632424.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632424.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560890
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT21310.37993189395425125No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT16010.28543921267984806No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG9290.16562962434701992No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT8470.15101000196116884No Hit
GTGTGGGGCTGCCTGCGGGCTGCGTCTAGTTGCAGTAGTTCTC8450.15065342580541638No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG8390.149583697338159No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG8170.1456613596248819No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT7960.141917309989481No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT7490.13353777032929806No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG6970.124266790279734No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT6880.12266219757884791No Hit
CATCAGAAGAGGCCATCAAGCAGGTCTTTGCGTTCCAAGGGCC6640.11838328370981832No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT6600.11767013139831341No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGTGGG6160.10982545597175916No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC5880.10483338979122465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2300.028.9565221
GTTTAGG701.9192703E-723.7857131
GCGGTAA802.719571E-823.12523
TAGACAG400.001929961123.1255
TGCATCA4950.022.7979814
TATATAC652.678782E-622.7692283
TAATACT1155.456968E-1222.521744
TATTGCG603.7219084E-521.5833329
CTTATAC4350.020.41379437
ATAATAC555.138583E-420.1818183
GCATCAG5700.020.12280815
GACAGGC5750.019.9478267
TTTAGGA656.894082E-519.9230752
TACACGG656.894082E-519.9230755
TATCTAT759.251906E-619.7333345
ATCAGAA5650.019.64601717
GCTAGAG851.2429828E-619.58823617
CCAGGAC7000.019.5571443
CGGTAAT951.6722879E-719.47368424
CATCAGA6100.019.40983616
Produced by FastQC (version 0.11.5)