##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632424.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	560890
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.08247428194477	34.0	33.0	34.0	31.0	34.0
2	33.226038973773825	34.0	33.0	34.0	31.0	34.0
3	33.30690688013693	34.0	34.0	34.0	31.0	34.0
4	36.58242614416374	37.0	37.0	37.0	35.0	37.0
5	36.55729109094475	37.0	37.0	37.0	35.0	37.0
6	36.58639483677727	37.0	37.0	37.0	35.0	37.0
7	36.58298775160905	37.0	37.0	37.0	35.0	37.0
8	36.55034142166913	37.0	37.0	37.0	35.0	37.0
9	38.352557542477136	39.0	39.0	39.0	37.0	39.0
10	38.38411631514201	39.0	39.0	39.0	37.0	39.0
11	38.45205833585908	39.0	39.0	39.0	37.0	39.0
12	38.42984720711726	39.0	39.0	39.0	37.0	39.0
13	38.445105100821905	39.0	39.0	39.0	37.0	39.0
14	40.048439087878194	41.0	40.0	41.0	38.0	41.0
15	40.03473051757029	41.0	40.0	41.0	38.0	41.0
16	40.028978944178	41.0	40.0	41.0	38.0	41.0
17	40.01275116332971	41.0	40.0	41.0	38.0	41.0
18	39.980345522294925	41.0	40.0	41.0	38.0	41.0
19	39.99014958369734	41.0	40.0	41.0	38.0	41.0
20	39.935028258660346	41.0	40.0	41.0	38.0	41.0
21	39.925429228547486	41.0	40.0	41.0	38.0	41.0
22	39.90301841715844	41.0	40.0	41.0	38.0	41.0
23	39.87416784039651	41.0	40.0	41.0	38.0	41.0
24	39.86246144520316	41.0	40.0	41.0	38.0	41.0
25	39.8120219650912	41.0	40.0	41.0	38.0	41.0
26	39.74161065449553	41.0	40.0	41.0	38.0	41.0
27	39.69708320704594	41.0	40.0	41.0	38.0	41.0
28	39.63471447164328	41.0	40.0	41.0	37.0	41.0
29	39.603610333576995	41.0	40.0	41.0	37.0	41.0
30	39.55288559254043	41.0	40.0	41.0	37.0	41.0
31	39.52273886145233	41.0	40.0	41.0	37.0	41.0
32	39.47688138494179	41.0	40.0	41.0	37.0	41.0
33	39.41590507942734	41.0	40.0	41.0	36.0	41.0
34	39.368095348464045	41.0	39.0	41.0	36.0	41.0
35	39.291957424807	41.0	39.0	41.0	36.0	41.0
36	39.25857654798624	41.0	39.0	41.0	35.0	41.0
37	39.21379058282373	41.0	39.0	41.0	35.0	41.0
38	39.14591809445702	41.0	39.0	41.0	35.0	41.0
39	39.086580256378255	41.0	39.0	41.0	35.0	41.0
40	38.97785483784699	41.0	39.0	41.0	35.0	41.0
41	38.93705182834424	40.0	39.0	41.0	35.0	41.0
42	38.8795164827328	40.0	39.0	41.0	35.0	41.0
43	38.17563693415821	40.0	38.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	8.0
19	18.0
20	35.0
21	46.0
22	100.0
23	205.0
24	332.0
25	457.0
26	630.0
27	818.0
28	1156.0
29	1629.0
30	2158.0
31	2860.0
32	3713.0
33	4927.0
34	7627.0
35	13440.0
36	20089.0
37	36384.0
38	98996.0
39	365258.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.82124837312129	15.735170889122644	11.428978944178002	29.014601793578066
2	19.91103424913976	17.824350585676335	34.18870010162421	28.075915063559698
3	20.53343792900569	20.17222628322844	27.561197382731017	31.733138405034854
4	15.415143789334806	13.949615789192176	32.171905364688264	38.46333505678475
5	16.8824546702562	34.035550642728516	32.81980423969049	16.262190447324787
6	38.3872060475316	32.240902850826366	14.2059940451782	15.165897056463834
7	33.023052648469395	26.94075487172173	19.530745779029758	20.50544670077912
8	28.56620727771934	32.92766852680561	18.29734885628198	20.208775339193068
9	28.3399597068944	13.164969958458878	16.53176202107365	41.96330831357307
10	18.55337053611225	24.509083777567795	30.732764000071317	26.20478168624864
11	39.04990283299756	19.7539624525308	19.242988821337516	21.953145893134128
12	24.746563497298936	22.414555438677816	26.83075112767209	26.008129936351153
13	32.583572536504484	17.320865053753856	22.09720266005812	27.998359749683537
14	24.472178145447415	17.843962274242724	22.955481466954307	34.72837811335556
15	28.35885824314928	24.05587548360641	20.143165326534614	27.44210094670969
16	27.291447520904278	23.31401879156341	20.535042521706572	28.85949116582574
17	26.556187487742694	23.795396601829236	22.632601757920447	27.015814152507623
18	26.448858064861202	22.28333541336091	23.654014156073384	27.613792365704505
19	28.324270356041293	21.975966767102285	22.349836866408744	27.349926010447685
20	26.99994651357664	22.933373745297654	23.058888552122518	27.007791189003193
21	28.564602685018453	22.264615165183905	21.91499224446861	27.25578990532903
22	28.280946353117365	23.061206297134913	21.623312949063095	27.034534400684628
23	27.603986521421316	23.28424468255808	22.045499117474016	27.066269678546597
24	28.480807288416628	22.61405979782132	21.432722993813407	27.472409919948653
25	27.751074185669207	22.284048565672414	22.85011321292945	27.114764035728932
26	28.267218171120895	22.464297812405285	22.61923015207973	26.64925386439409
27	28.584749237818468	22.242507443527252	21.911069906755333	27.261673411898947
28	26.836278058086254	22.271568400221078	23.38961293658293	27.502540605109736
29	27.901371035318867	22.206849827952006	23.0125336518747	26.87924548485443
30	26.80810854178181	22.380502415803456	23.118080193977427	27.693308848437304
31	26.826293925725185	22.40956337249728	22.84209024942502	27.922052452352514
32	25.92986147016349	22.59409153309918	23.336483089375815	28.139563907361513
33	27.10014441334308	22.62065645670274	23.53866176968746	26.74053736026672
34	27.31266380217155	22.5789370464797	23.40441084704666	26.70398830430209
35	27.52357859829913	22.65667064843374	23.349676407138656	26.470074346128474
36	27.30267966981048	22.305443135017562	22.48961471946371	27.902262475708252
37	26.077305710567135	22.16780473889711	23.58127262029988	28.173616930235873
38	26.887803312592485	21.76023819287204	24.297990693362333	27.053967801173133
39	25.462390129972007	21.318083759738986	25.29711708178074	27.922409028508266
40	25.56169658934907	20.956158961650235	26.599154914510866	26.882989534489827
41	24.475209042771308	20.568560680347307	26.967319795325285	27.9889104815561
42	23.669168642692863	21.152632423469843	27.129205370036907	28.04899356380039
43	23.73460036727344	20.16045927008861	28.163811085952684	27.941129276685267
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	7.0
2	5.0
3	17.0
4	29.0
5	29.0
6	30.5
7	32.0
8	35.5
9	39.0
10	48.0
11	57.0
12	57.0
13	103.0
14	149.0
15	241.5
16	334.0
17	368.0
18	402.0
19	402.0
20	507.0
21	612.0
22	791.5
23	971.0
24	1259.0
25	1547.0
26	1547.0
27	1998.0
28	2449.0
29	3399.5
30	4350.0
31	5437.0
32	6524.0
33	6524.0
34	8370.5
35	10217.0
36	12048.5
37	13880.0
38	16888.0
39	19896.0
40	19896.0
41	22264.5
42	24633.0
43	27055.5
44	29478.0
45	31304.5
46	33131.0
47	33131.0
48	35378.5
49	37626.0
50	40997.0
51	44368.0
52	49134.5
53	53901.0
54	53901.0
55	52759.5
56	51618.0
57	48071.5
58	44525.0
59	42017.0
60	39509.0
61	39509.0
62	39001.0
63	38493.0
64	32124.0
65	25755.0
66	22453.0
67	19151.0
68	19151.0
69	16633.0
70	14115.0
71	12815.5
72	11516.0
73	12161.0
74	12806.0
75	12806.0
76	12754.5
77	12703.0
78	8277.0
79	3851.0
80	2460.0
81	1069.0
82	1069.0
83	834.5
84	600.0
85	450.5
86	301.0
87	215.5
88	130.0
89	130.0
90	100.0
91	70.0
92	46.5
93	23.0
94	16.0
95	9.0
96	9.0
97	6.5
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	560890.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.65085975228659
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.32117316447594	47.72177188016906
2	6.3171378562284	6.904740300331973
3	1.9558874353954596	3.206727897691702
4	1.025978473155791	2.242824225812091
5	0.6253077845801558	1.7086804018551567
6	0.40237408553462795	1.3194053829904515
7	0.30764960234915645	1.1769320689581344
8	0.2367147473680861	1.034933156776898
9	0.1944390688568876	0.9563636054216667
>10	1.3609348588523984	14.637569967176049
>50	0.14226346620148536	5.410666139036121
>100	0.1039113329451386	10.886002968854507
>500	0.005572532050685667	2.1248190829493527
>1k	6.555920059630197E-4	0.6685629219768134
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT	2131	0.37993189395425125	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT	1601	0.28543921267984806	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	929	0.16562962434701992	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	847	0.15101000196116884	No Hit
GTGTGGGGCTGCCTGCGGGCTGCGTCTAGTTGCAGTAGTTCTC	845	0.15065342580541638	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	839	0.149583697338159	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	817	0.1456613596248819	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	796	0.141917309989481	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	749	0.13353777032929806	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	697	0.124266790279734	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	688	0.12266219757884791	No Hit
CATCAGAAGAGGCCATCAAGCAGGTCTTTGCGTTCCAAGGGCC	664	0.11838328370981832	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	660	0.11767013139831341	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGTGGG	616	0.10982545597175916	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	588	0.10483338979122465	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	1.7828807787623242E-4	0.0	0.0	0.0	0.0
8	1.7828807787623242E-4	0.0	0.0	1.7828807787623242E-4	0.0
9	1.7828807787623242E-4	0.0	0.0	1.7828807787623242E-4	0.0
10	1.7828807787623242E-4	0.0	0.0	1.7828807787623242E-4	0.0
11	1.7828807787623242E-4	0.0	0.0	1.7828807787623242E-4	0.0
12	1.7828807787623242E-4	0.0	0.0	1.7828807787623242E-4	0.0
13	1.7828807787623242E-4	0.0	0.0	1.7828807787623242E-4	0.0
14	1.7828807787623242E-4	0.0	0.0	1.7828807787623242E-4	0.0
15	1.7828807787623242E-4	0.0	0.0	1.7828807787623242E-4	0.0
16	1.7828807787623242E-4	0.0	0.0	1.7828807787623242E-4	0.0
17	1.7828807787623242E-4	0.0	0.0	3.5657615575246485E-4	0.0
18	1.7828807787623242E-4	0.0	0.0	7.131523115049297E-4	0.0
19	1.7828807787623242E-4	0.0	0.0	8.91440389381162E-4	0.0
20	1.7828807787623242E-4	0.0	0.0	0.0019611688566385566	0.0
21	5.348642336286972E-4	0.0	0.0	0.003030897323895951	1.7828807787623242E-4
22	7.131523115049297E-4	0.0	0.0	0.003922337713277113	1.7828807787623242E-4
23	7.131523115049297E-4	0.0	0.0	0.00517035425841074	1.7828807787623242E-4
24	7.131523115049297E-4	0.0	0.0	0.006953235037173064	1.7828807787623242E-4
25	7.131523115049297E-4	0.0	0.0	0.007309811192925529	1.7828807787623242E-4
26	7.131523115049297E-4	0.0	0.0	0.009984132361069015	1.7828807787623242E-4
27	7.131523115049297E-4	0.0	0.0	0.021929433578776587	3.5657615575246485E-4
28	7.131523115049297E-4	0.0	0.0	0.08022963504430458	3.5657615575246485E-4
29	7.131523115049297E-4	0.0	0.0	0.1700868262939257	3.5657615575246485E-4
30	7.131523115049297E-4	0.0	0.0	0.27581165647453154	3.5657615575246485E-4
31	7.131523115049297E-4	0.0	0.0	0.6471857226907237	3.5657615575246485E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	230	0.0	28.956522	1
GTTTAGG	70	1.9192703E-7	23.785713	1
GCGGTAA	80	2.719571E-8	23.125	23
TAGACAG	40	0.0019299611	23.125	5
TGCATCA	495	0.0	22.79798	14
TATATAC	65	2.678782E-6	22.769228	3
TAATACT	115	5.456968E-12	22.52174	4
TATTGCG	60	3.7219084E-5	21.583332	9
CTTATAC	435	0.0	20.413794	37
ATAATAC	55	5.138583E-4	20.181818	3
GCATCAG	570	0.0	20.122808	15
GACAGGC	575	0.0	19.947826	7
TTTAGGA	65	6.894082E-5	19.923075	2
TACACGG	65	6.894082E-5	19.923075	5
TATCTAT	75	9.251906E-6	19.733334	5
ATCAGAA	565	0.0	19.646017	17
GCTAGAG	85	1.2429828E-6	19.588236	17
CCAGGAC	700	0.0	19.557144	3
CGGTAAT	95	1.6722879E-7	19.473684	24
CATCAGA	610	0.0	19.409836	16
>>END_MODULE