FastQCFastQC Report
Fri 10 Feb 2017
ERR1632423.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632423.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25065
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA740.2952323957709954No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA670.26730500698184717No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT670.26730500698184717RNA PCR Primer, Index 27 (95% over 23bp)
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA600.23937761819269898No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA510.20347097546379417No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT490.19549172152403752No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA460.18352284061440255No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA410.16357470576501096No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT400.15958507879513265No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC360.1436265709156194No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT360.1436265709156194No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC350.13963694394574108No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG350.13963694394574108No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA330.13165769000598446No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC300.11968880909634949No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC270.10771992818671454No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG260.10373030121683623No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC260.10373030121683623No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA260.10373030121683623No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA260.10373030121683623No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA400.001879237823.1251
CTCTTAT603.542623E-521.58333437
TCTCTTA1101.3425712E-515.13636437
GTCTCTT1706.372003E-510.88235337
CTGTCTC3053.3778633E-910.31147637
CCTGTCT1700.0087350028.70588236
TGTCTCT2208.5367815E-48.40909136