##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632423.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25065
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.02968282465589	34.0	33.0	34.0	31.0	34.0
2	33.16197885497706	34.0	33.0	34.0	31.0	34.0
3	33.244763614602036	34.0	34.0	34.0	31.0	34.0
4	36.51234789547178	37.0	37.0	37.0	35.0	37.0
5	36.49331737482545	37.0	37.0	37.0	35.0	37.0
6	36.517295032914426	37.0	37.0	37.0	35.0	37.0
7	36.53724316776381	37.0	37.0	37.0	35.0	37.0
8	36.50305206463196	37.0	37.0	37.0	35.0	37.0
9	38.2754837422701	39.0	39.0	39.0	37.0	39.0
10	38.32092559345701	39.0	39.0	39.0	37.0	39.0
11	38.388749251944944	39.0	39.0	39.0	37.0	39.0
12	38.35208458009176	39.0	39.0	39.0	37.0	39.0
13	38.37330939557151	39.0	39.0	39.0	37.0	39.0
14	39.93145820865749	41.0	40.0	41.0	38.0	41.0
15	39.90867743865949	41.0	40.0	41.0	38.0	41.0
16	39.88430081787353	41.0	40.0	41.0	38.0	41.0
17	39.876999800518654	41.0	40.0	41.0	38.0	41.0
18	39.8852184320766	41.0	40.0	41.0	38.0	41.0
19	39.9149411530022	41.0	40.0	41.0	38.0	41.0
20	39.86315579493317	41.0	40.0	41.0	38.0	41.0
21	39.82158388190704	41.0	40.0	41.0	38.0	41.0
22	39.80271294633952	41.0	40.0	41.0	38.0	41.0
23	39.76365449830441	41.0	40.0	41.0	38.0	41.0
24	39.74282864552164	41.0	40.0	41.0	38.0	41.0
25	39.68813085976461	41.0	40.0	41.0	37.0	41.0
26	39.609974067424695	41.0	40.0	41.0	37.0	41.0
27	39.5211649710752	41.0	40.0	41.0	37.0	41.0
28	39.474566128067025	41.0	40.0	41.0	37.0	41.0
29	39.444524236983845	41.0	39.0	41.0	37.0	41.0
30	39.39457410732096	41.0	39.0	41.0	36.0	41.0
31	39.33042090564532	41.0	39.0	41.0	36.0	41.0
32	39.262158388190706	41.0	39.0	41.0	36.0	41.0
33	39.17406742469579	41.0	39.0	41.0	35.0	41.0
34	39.14781567923399	41.0	39.0	41.0	35.0	41.0
35	39.10317175344105	41.0	39.0	41.0	35.0	41.0
36	39.05186515060842	41.0	39.0	41.0	35.0	41.0
37	38.99936165968482	41.0	39.0	41.0	35.0	41.0
38	38.93676441252743	41.0	39.0	41.0	35.0	41.0
39	38.807699980051865	40.0	39.0	41.0	35.0	41.0
40	38.715220426890085	40.0	38.0	41.0	35.0	41.0
41	38.67500498703371	40.0	38.0	41.0	35.0	41.0
42	38.61715539597048	40.0	38.0	41.0	35.0	41.0
43	37.88158787153401	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	1.0
21	3.0
22	4.0
23	8.0
24	17.0
25	27.0
26	33.0
27	49.0
28	67.0
29	97.0
30	96.0
31	178.0
32	211.0
33	295.0
34	382.0
35	642.0
36	979.0
37	1884.0
38	4817.0
39	15273.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.59724715739078	16.540993417115498	12.483542788749252	28.37821663674446
2	21.867145421903054	18.563734290843804	31.577897466586872	27.991222820666266
3	20.80590464791542	19.581089168162777	28.649511270696188	30.963494913225613
4	15.727109515260324	15.627368841013364	33.80410931577897	34.84141232794734
5	16.860163574705762	33.01815280271295	33.55675244364652	16.56493117893477
6	34.83343307400758	34.52623179732695	14.633951725513663	16.006383403151805
7	31.23478954717734	27.069618990624377	19.52523439058448	22.170357071613804
8	27.620187512467588	30.193496908039098	20.29124276880112	21.895072810692202
9	28.023139836425294	12.387791741472173	17.318970676241772	42.27009774586076
10	18.962696987831638	22.94035507680032	29.47935368043088	28.617594254937163
11	40.22341911031319	18.99860363056054	18.34031518053062	22.43766207859565
12	22.98424097346898	23.60662278077	26.933971673648514	26.475164572112508
13	35.60742070616397	17.48254538200678	19.868342309994016	27.041691601835225
14	24.88330341113106	19.249950129662878	22.33792140434869	33.52882505485737
15	29.52722920406942	23.69040494713744	19.988031119090365	26.794334729702772
16	26.27568322361859	23.774187113504887	21.77937362856573	28.17075603431079
17	26.957909435467787	23.470975463794137	21.488130859764613	28.08298424097347
18	26.574905246359464	21.508078994614007	22.75284260921604	29.16417314981049
19	28.729303810093754	22.018751246758427	22.90843806104129	26.34350688210652
20	30.165569519249953	20.630361061240773	22.17833632555356	27.02573309395572
21	27.743865948533813	21.70756034310792	21.715539597047677	28.83303411131059
22	28.87692000797926	22.32994215040894	20.86973867943347	27.923399162178335
23	28.469978057051666	21.35248354278875	22.22222222222222	27.955316177937362
24	27.903451027328945	21.54797526431279	21.508078994614007	29.040494713744263
25	28.50987432675045	21.70756034310792	22.50947536405346	27.273089966088172
26	28.473967684021545	22.206263714342708	22.03869938160782	27.281069220027927
27	26.973867943347297	21.895072810692202	23.582685018950727	27.548374227009774
28	26.89806503091961	22.513464991023337	21.915020945541592	28.673449032515457
29	27.723917813684416	23.020147616197885	21.926989826451226	27.328944743666465
30	26.58288450029922	21.883103929782564	23.486933971673647	28.047077598244563
31	27.7279074406543	22.577299022541393	22.16237781767405	27.53241571913026
32	26.25174546179932	22.06263714342709	22.549371633752244	29.136245761021346
33	26.02034709754638	22.050668262517455	24.623977658088968	27.305006981847196
34	26.866148015160583	21.54797526431279	24.025533612607223	27.56034310791941
35	26.423299421504087	22.932375822860564	24.205066826251745	26.439257929383604
36	26.59086375423898	21.75144623977658	24.424496309595053	27.233193696389385
37	26.646718531817275	21.675643327348894	24.117295032914424	27.56034310791941
38	25.864751645721125	20.670257330939556	25.86076201875125	27.60422900458807
39	25.46179932176342	19.940155595451824	26.459206064232994	28.138839018551764
40	24.484340714143226	20.95352084580092	27.648114901256733	26.91402353879912
41	23.69040494713744	20.530620386993817	28.19868342309994	27.5802912427688
42	21.78336325553561	21.228805106722522	29.682824655894674	27.305006981847196
43	21.480151605824854	21.053261520047876	29.024536205864752	28.44205066826252
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.0
6	2.5
7	3.0
8	2.5
9	2.0
10	3.0
11	4.0
12	4.0
13	8.5
14	13.0
15	18.5
16	24.0
17	26.5
18	29.0
19	29.0
20	35.0
21	41.0
22	30.0
23	19.0
24	28.0
25	37.0
26	37.0
27	47.5
28	58.0
29	83.0
30	108.0
31	128.0
32	148.0
33	148.0
34	204.5
35	261.0
36	333.0
37	405.0
38	501.5
39	598.0
40	598.0
41	713.0
42	828.0
43	953.5
44	1079.0
45	1401.5
46	1724.0
47	1724.0
48	1927.5
49	2131.0
50	2366.5
51	2602.0
52	2690.0
53	2778.0
54	2778.0
55	2598.5
56	2419.0
57	2380.5
58	2342.0
59	2226.0
60	2110.0
61	2110.0
62	1901.0
63	1692.0
64	1406.5
65	1121.0
66	989.0
67	857.0
68	857.0
69	733.5
70	610.0
71	504.0
72	398.0
73	315.5
74	233.0
75	233.0
76	190.5
77	148.0
78	119.5
79	91.0
80	79.0
81	67.0
82	67.0
83	55.0
84	43.0
85	33.0
86	23.0
87	16.0
88	9.0
89	9.0
90	8.0
91	7.0
92	4.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	25065.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.50109714741671
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.10169491525424	68.92878515858767
2	5.241199478487614	8.019150209455415
3	1.6427640156453716	3.7701974865350087
4	0.938722294654498	2.872531418312388
5	0.5267275097783573	2.01476161978855
6	0.30247718383311606	1.3883901855176541
7	0.22946544980443287	1.2288051067225214
8	0.20860495436766624	1.2766806303610612
9	0.14602346805736635	1.0053859964093357
>10	0.636245110821382	8.222621184919209
>50	0.02607561929595828	1.272691003391183
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	74	0.2952323957709954	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	67	0.26730500698184717	No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT	67	0.26730500698184717	RNA PCR Primer, Index 27 (95% over 23bp)
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	60	0.23937761819269898	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	51	0.20347097546379417	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	49	0.19549172152403752	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	46	0.18352284061440255	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	41	0.16357470576501096	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	40	0.15958507879513265	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	36	0.1436265709156194	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	36	0.1436265709156194	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	35	0.13963694394574108	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	35	0.13963694394574108	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	33	0.13165769000598446	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	30	0.11968880909634949	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	27	0.10771992818671454	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	26	0.10373030121683623	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	26	0.10373030121683623	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	26	0.10373030121683623	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	26	0.10373030121683623	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.003989626969878316	0.0
22	0.0	0.0	0.0	0.011968880909634948	0.0
23	0.0	0.0	0.0	0.015958507879513265	0.0
24	0.0	0.0	0.0	0.01994813484939158	0.0
25	0.0	0.0	0.0	0.01994813484939158	0.0
26	0.0	0.0	0.0	0.01994813484939158	0.0
27	0.0	0.0	0.0	0.03590664272890485	0.0
28	0.0	0.0	0.0	0.13564731697586277	0.0
29	0.0	0.0	0.0	0.27528426092160385	0.0
30	0.0	0.0	0.0	0.4867344903251546	0.0
31	0.0	0.0	0.0	0.9615000997406743	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	40	0.0018792378	23.125	1
CTCTTAT	60	3.542623E-5	21.583334	37
TCTCTTA	110	1.3425712E-5	15.136364	37
GTCTCTT	170	6.372003E-5	10.882353	37
CTGTCTC	305	3.3778633E-9	10.311476	37
CCTGTCT	170	0.008735002	8.705882	36
TGTCTCT	220	8.5367815E-4	8.409091	36
>>END_MODULE