FastQCFastQC Report
Fri 10 Feb 2017
ERR1632422.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632422.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42141
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1110.2634014380294725No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT880.20882276168102323Illumina PCR Primer Index 10 (95% over 22bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA870.20644977575282977No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA860.20407678982463634No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA720.17085498682992808No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG650.15424408533257397No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT630.14949811347618708No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC610.14475214161980018No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA600.14237915569160675No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT600.14237915569160675No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC570.1352601979070264No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC560.13288721197883296No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC510.12102228233786573No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT500.1186492964096723No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA490.11627631048147884No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT480.1139033245532854No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA480.1139033245532854No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCGG200.001821870837.020
GCCGGAA200.001821870837.027
TGGGAAA200.001821870837.05
TCTGGGA250.0054371529.63
GGTATCA453.889065E-628.7777771
GTATCAA656.702542E-519.9230772
TCTTTAG656.702542E-519.9230771
CTTTAGT701.1841892E-418.52
TTTAGTG701.1841892E-418.53
AGTGACC500.00692564618.56
TCACTAA609.036955E-418.531
CTTATAC803.2868062E-416.187537
AGTACGG1058.923314E-615.85714412
GAGTACG1058.923314E-615.85714411
TATCAAC1058.923314E-615.8571441
ACTTCAT700.002537307315.85714322
AACGCAG1101.3869385E-515.1363645
ACTGTCT1001.0541924E-414.837
GTCACTA750.004017751714.830
ACGCAGA1152.1115817E-514.4782616