##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632422.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	42141
Sequences flagged as poor quality	0
Sequence length	43
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.98761301345483	34.0	33.0	34.0	31.0	34.0
2	33.124534301511595	34.0	33.0	34.0	31.0	34.0
3	33.2102940129565	34.0	33.0	34.0	31.0	34.0
4	36.52234166251394	37.0	37.0	37.0	35.0	37.0
5	36.470017322797275	37.0	37.0	37.0	35.0	37.0
6	36.51299209795686	37.0	37.0	37.0	35.0	37.0
7	36.510595382169384	37.0	37.0	37.0	35.0	37.0
8	36.477610877767496	37.0	37.0	37.0	35.0	37.0
9	38.281720889395125	39.0	39.0	39.0	37.0	39.0
10	38.29216202747918	39.0	39.0	39.0	37.0	39.0
11	38.382382952469094	39.0	39.0	39.0	37.0	39.0
12	38.317268218599466	39.0	39.0	39.0	37.0	39.0
13	38.36040910277402	39.0	39.0	39.0	37.0	39.0
14	39.897605657198454	41.0	40.0	41.0	38.0	41.0
15	39.89304952421632	41.0	40.0	41.0	38.0	41.0
16	39.84867468735911	41.0	40.0	41.0	38.0	41.0
17	39.840725184499654	41.0	40.0	41.0	38.0	41.0
18	39.84993236990105	41.0	40.0	41.0	38.0	41.0
19	39.84995609976033	41.0	40.0	41.0	38.0	41.0
20	39.82662964808619	41.0	40.0	41.0	38.0	41.0
21	39.78885171210935	41.0	40.0	41.0	38.0	41.0
22	39.7492465769678	41.0	40.0	41.0	38.0	41.0
23	39.689471061436606	41.0	40.0	41.0	38.0	41.0
24	39.66367670439714	41.0	40.0	41.0	37.0	41.0
25	39.62611233715384	41.0	40.0	41.0	37.0	41.0
26	39.53494221779265	41.0	40.0	41.0	37.0	41.0
27	39.45307420326998	41.0	39.0	41.0	37.0	41.0
28	39.38857644574168	41.0	39.0	41.0	36.0	41.0
29	39.331150186279395	41.0	39.0	41.0	36.0	41.0
30	39.28850762914976	41.0	39.0	41.0	36.0	41.0
31	39.20165634417788	41.0	39.0	41.0	36.0	41.0
32	39.152654184760685	41.0	39.0	41.0	35.0	41.0
33	39.07783393844475	41.0	39.0	41.0	35.0	41.0
34	39.038561021333145	41.0	39.0	41.0	35.0	41.0
35	38.934315749507604	40.0	39.0	41.0	35.0	41.0
36	38.89338174224627	40.0	39.0	41.0	35.0	41.0
37	38.8255618044185	40.0	38.0	41.0	35.0	41.0
38	38.745568448779096	40.0	38.0	41.0	35.0	41.0
39	38.64635390712133	40.0	38.0	41.0	35.0	41.0
40	38.567713153461	40.0	38.0	41.0	35.0	41.0
41	38.49816093590565	40.0	38.0	41.0	35.0	41.0
42	38.41024180726608	40.0	38.0	41.0	35.0	41.0
43	37.64604541895066	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	0.0
18	3.0
19	0.0
20	4.0
21	5.0
22	13.0
23	10.0
24	26.0
25	49.0
26	50.0
27	83.0
28	123.0
29	138.0
30	208.0
31	279.0
32	365.0
33	515.0
34	763.0
35	1143.0
36	1742.0
37	3557.0
38	8797.0
39	24267.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.22156569611542	15.139650221874184	12.144941980494057	29.49384210151634
2	21.724686172610998	18.77269167793835	30.350490021594172	29.152132127856483
3	21.091098929783346	18.817778410574025	28.032082769749177	32.059039889893455
4	15.98443321231105	14.553522697610402	32.71872997793123	36.74331411214731
5	17.49839823449847	32.6238105408035	32.460074511758144	17.417716712939892
6	36.84297952113144	32.31532237013835	14.895232671270259	15.946465437459956
7	31.615291521321275	26.84796279158065	18.782183621651125	22.75456206544695
8	28.995515056595718	29.783346384755937	19.375430103699482	21.845708454948863
9	28.670415984433212	12.325288911036758	16.07223369165421	42.932061412875825
10	19.712394105502952	22.61692888161173	28.525663842813415	29.145013170071906
11	41.24961438978667	17.930281673429675	17.692983080610333	23.12712085617332
12	24.064450297809735	22.837616572933722	25.447901093946513	27.65003203531003
13	36.25685199686765	16.276310481478845	19.605609738734252	27.861227782919247
14	25.030255570584465	17.970622434208966	21.45179279086875	35.54732920433782
15	30.407441683870818	23.065423222040295	18.2838565767305	28.243278517358394
16	27.56223155596687	22.927790038205075	20.735151040554328	28.774827365273726
17	28.490069053890508	22.476922711848317	19.985287487245202	29.04772074701597
18	27.934790346693244	20.068341994731973	21.95486580764576	30.042001850929022
19	29.292138297619896	21.214494198049405	21.587052988775778	27.906314515554925
20	31.8264872689305	19.733750978856694	20.768372843549038	27.671388908663776
21	28.89822263353978	20.732778054626134	20.19173726299803	30.177262048836052
22	29.918606582662967	21.21212121212121	19.71714007735934	29.152132127856483
23	29.68130798984362	20.98194157708645	20.201229206710806	29.135521226359128
24	29.292138297619896	20.538193208514276	20.61887473007285	29.55079376379298
25	29.790465342540518	20.504971405519566	21.12669371870625	28.577869533233667
26	29.052466718872356	21.380603213022948	20.71379416720059	28.85313590090411
27	28.43549037754206	20.493106475878598	22.301321753162004	28.770081393417335
28	27.678507866448353	21.603663890273133	21.1433046202036	29.574523623074917
29	28.535155786526182	22.491160627417482	20.79922166061555	28.17446192544078
30	27.963266177831564	20.889395125886903	22.196910372321494	28.950428323960036
31	28.822287083837594	21.750789017821123	21.083979971998765	28.342943926342517
32	26.895422510144513	20.9985524785838	21.96198476543034	30.144040245841342
33	26.70083766403265	20.711421181272396	23.492560689115113	29.09518046557984
34	27.43646330177262	21.064996084573217	22.40810611993071	29.090434493723453
35	26.340143802947246	21.992833582496857	23.850881564272324	27.816141050283573
36	27.094753328112763	20.89414109774329	23.77257302864194	28.238532545502004
37	27.168315891886763	20.675826392349496	23.84138962055955	28.31446809520419
38	26.60829121283311	20.16326143185971	24.77871906219596	28.44972829311122
39	25.682826700837662	19.131012553095562	26.07911535084597	29.107045395220805
40	25.035001542440856	19.610355710590635	27.775800289504282	27.578842457464226
41	23.793929901995682	19.064568947106146	28.528036828741605	28.613464322156567
42	22.510144514843027	19.448992667473483	29.65995111648988	28.38091170119361
43	21.12669371870625	19.883249092332882	29.510453003013694	29.479604185947178
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.0
6	2.0
7	2.0
8	1.5
9	1.0
10	1.5
11	2.0
12	2.0
13	6.5
14	11.0
15	14.5
16	18.0
17	18.0
18	18.0
19	18.0
20	24.5
21	31.0
22	25.5
23	20.0
24	30.5
25	41.0
26	41.0
27	52.5
28	64.0
29	90.5
30	117.0
31	155.5
32	194.0
33	194.0
34	233.0
35	272.0
36	332.5
37	393.0
38	543.5
39	694.0
40	694.0
41	841.0
42	988.0
43	1197.0
44	1406.0
45	1894.5
46	2383.0
47	2383.0
48	2792.5
49	3202.0
50	3662.5
51	4123.0
52	4436.0
53	4749.0
54	4749.0
55	4471.5
56	4194.0
57	4074.5
58	3955.0
59	3895.0
60	3835.0
61	3835.0
62	3591.5
63	3348.0
64	2880.5
65	2413.0
66	2099.5
67	1786.0
68	1786.0
69	1568.5
70	1351.0
71	1166.0
72	981.0
73	810.5
74	640.0
75	640.0
76	509.0
77	378.0
78	302.5
79	227.0
80	180.5
81	134.0
82	134.0
83	106.0
84	78.0
85	56.5
86	35.0
87	31.0
88	27.0
89	27.0
90	21.0
91	15.0
92	12.0
93	9.0
94	6.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	42141.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.55209890605349
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.41655791450489	66.66192069481028
2	5.4811089537511535	8.172563536698227
3	1.8652321991278606	4.171709261764078
4	0.983543941178343	2.9330106072470987
5	0.6365980201801572	2.3729859281934456
6	0.3915077824107967	1.751263615006763
7	0.2259922971639558	1.1793740063121425
8	0.18143043575134482	1.0820815832562112
9	0.11140465353152752	0.7474905673809354
>10	0.6652449310882643	8.751572103177429
>50	0.03819588121080943	1.9126266581239175
>100	0.0031829901009007865	0.2634014380294725
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	111	0.2634014380294725	No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT	88	0.20882276168102323	Illumina PCR Primer Index 10 (95% over 22bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	87	0.20644977575282977	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	86	0.20407678982463634	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	72	0.17085498682992808	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	65	0.15424408533257397	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	63	0.14949811347618708	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	61	0.14475214161980018	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	60	0.14237915569160675	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	60	0.14237915569160675	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	57	0.1352601979070264	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	56	0.13288721197883296	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	51	0.12102228233786573	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	50	0.1186492964096723	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	49	0.11627631048147884	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	48	0.1139033245532854	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	48	0.1139033245532854	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.002372985928193446	0.0
22	0.0	0.0	0.0	0.004745971856386892	0.0
23	0.0	0.0	0.0	0.007118957784580338	0.0
24	0.0	0.0	0.0	0.007118957784580338	0.0
25	0.0	0.0	0.0	0.009491943712773784	0.0
26	0.0	0.0	0.0	0.009491943712773784	0.0
27	0.0	0.0	0.0	0.047459718563868915	0.0
28	0.0	0.0	0.0	0.15899005718896086	0.0
29	0.0	0.0	0.0	0.35832087515721034	0.0
30	0.0	0.0	0.0	0.6407062006122304	0.0
31	0.0	0.0	0.0	1.1936119218813033	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCTCGG	20	0.0018218708	37.0	20
GCCGGAA	20	0.0018218708	37.0	27
TGGGAAA	20	0.0018218708	37.0	5
TCTGGGA	25	0.00543715	29.6	3
GGTATCA	45	3.889065E-6	28.777777	1
GTATCAA	65	6.702542E-5	19.923077	2
TCTTTAG	65	6.702542E-5	19.923077	1
CTTTAGT	70	1.1841892E-4	18.5	2
TTTAGTG	70	1.1841892E-4	18.5	3
AGTGACC	50	0.006925646	18.5	6
TCACTAA	60	9.036955E-4	18.5	31
CTTATAC	80	3.2868062E-4	16.1875	37
AGTACGG	105	8.923314E-6	15.857144	12
GAGTACG	105	8.923314E-6	15.857144	11
TATCAAC	105	8.923314E-6	15.857144	1
ACTTCAT	70	0.0025373073	15.857143	22
AACGCAG	110	1.3869385E-5	15.136364	5
ACTGTCT	100	1.0541924E-4	14.8	37
GTCACTA	75	0.0040177517	14.8	30
ACGCAGA	115	2.1115817E-5	14.478261	6
>>END_MODULE