FastQCFastQC Report
Fri 10 Feb 2017
ERR1632421.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632421.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3568
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA110.30829596412556054No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT100.2802690582959641RNA PCR Primer, Index 26 (100% over 22bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA90.2522421524663677No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT80.2242152466367713No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC70.1961883408071749No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA70.1961883408071749No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC70.1961883408071749No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG70.1961883408071749No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC60.1681614349775785No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC60.1681614349775785No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG60.1681614349775785No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC60.1681614349775785No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA60.1681614349775785No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT50.14013452914798205No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC50.14013452914798205No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA50.14013452914798205No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT50.14013452914798205No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA50.14013452914798205No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA50.14013452914798205No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT50.14013452914798205No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA50.14013452914798205No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC50.14013452914798205No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC50.14013452914798205No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT50.14013452914798205No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA50.14013452914798205No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC40.11210762331838565No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG40.11210762331838565No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT40.11210762331838565No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC40.11210762331838565No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT40.11210762331838565No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC40.11210762331838565No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA40.11210762331838565No Hit
GTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT40.11210762331838565No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC40.11210762331838565No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG40.11210762331838565No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA40.11210762331838565No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG40.11210762331838565No Hit
CTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTT40.11210762331838565No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA40.11210762331838565No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)