FastQCFastQC Report
Fri 10 Feb 2017
ERR1632420.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632420.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4255
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT280.6580493537015276TruSeq Adapter, Index 12 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA130.30552291421856637No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA110.25851938895417154No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT90.21151586368977673No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC80.18801410105757932No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT80.18801410105757932No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA80.18801410105757932No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC80.18801410105757932No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG80.18801410105757932No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA80.18801410105757932No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA80.18801410105757932No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT60.1410105757931845No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA60.1410105757931845No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT60.1410105757931845No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC50.11750881316098707No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT50.11750881316098707No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG50.11750881316098707No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA50.11750881316098707No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA50.11750881316098707No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA50.11750881316098707No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC50.11750881316098707No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA50.11750881316098707No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC50.11750881316098707No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC50.11750881316098707RNA PCR Primer, Index 12 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)