##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632420.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4255
Sequences flagged as poor quality	0
Sequence length	43
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.84535840188014	34.0	31.0	34.0	31.0	34.0
2	32.95370152761457	34.0	33.0	34.0	31.0	34.0
3	33.06509988249119	34.0	33.0	34.0	31.0	34.0
4	36.39647473560517	37.0	37.0	37.0	35.0	37.0
5	36.342655699177435	37.0	37.0	37.0	35.0	37.0
6	36.39764982373678	37.0	37.0	37.0	35.0	37.0
7	36.36075205640423	37.0	37.0	37.0	35.0	37.0
8	36.3572267920094	37.0	37.0	37.0	35.0	37.0
9	38.07356051703878	39.0	39.0	39.0	37.0	39.0
10	38.17931844888366	39.0	39.0	39.0	37.0	39.0
11	38.24324324324324	39.0	39.0	39.0	37.0	39.0
12	38.21410105757932	39.0	39.0	39.0	37.0	39.0
13	38.23924794359577	39.0	39.0	39.0	37.0	39.0
14	39.74383078730905	41.0	40.0	41.0	38.0	41.0
15	39.790129259694474	41.0	40.0	41.0	38.0	41.0
16	39.70199764982374	41.0	40.0	41.0	37.0	41.0
17	39.65569917743831	41.0	40.0	41.0	37.0	41.0
18	39.736545240893065	41.0	40.0	41.0	38.0	41.0
19	39.611515863689775	41.0	40.0	41.0	37.0	41.0
20	39.58096357226792	41.0	40.0	41.0	37.0	41.0
21	39.55981198589894	41.0	39.0	41.0	37.0	41.0
22	39.541950646298474	41.0	39.0	41.0	37.0	41.0
23	39.521974148061105	41.0	40.0	41.0	37.0	41.0
24	39.4951821386604	41.0	39.0	41.0	37.0	41.0
25	39.44794359576968	41.0	39.0	41.0	37.0	41.0
26	39.36051703877791	41.0	39.0	41.0	36.0	41.0
27	39.21786133960047	41.0	39.0	41.0	36.0	41.0
28	39.20587544065805	41.0	39.0	41.0	35.0	41.0
29	39.17344300822562	41.0	39.0	41.0	35.0	41.0
30	39.01081081081081	41.0	39.0	41.0	35.0	41.0
31	38.99200940070505	40.0	39.0	41.0	35.0	41.0
32	38.90176263219742	40.0	39.0	41.0	35.0	41.0
33	38.786133960047	40.0	38.0	41.0	35.0	41.0
34	38.781198589894245	40.0	38.0	41.0	35.0	41.0
35	38.74829612220917	40.0	38.0	41.0	35.0	41.0
36	38.67426556991774	40.0	38.0	41.0	35.0	41.0
37	38.66298472385429	40.0	38.0	41.0	35.0	41.0
38	38.5283196239718	40.0	38.0	41.0	35.0	41.0
39	38.38119858989424	40.0	38.0	41.0	34.0	41.0
40	38.32996474735605	40.0	37.0	41.0	34.0	41.0
41	38.22937720329025	40.0	37.0	41.0	34.0	41.0
42	38.18448883666275	40.0	37.0	41.0	34.0	41.0
43	37.31680376028202	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	0.0
23	2.0
24	5.0
25	5.0
26	6.0
27	9.0
28	16.0
29	19.0
30	28.0
31	45.0
32	46.0
33	69.0
34	83.0
35	135.0
36	230.0
37	384.0
38	881.0
39	2291.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.30082256169213	15.417156286721504	12.314923619271445	27.967097532314924
2	22.232667450058756	17.391304347826086	30.8813160987074	29.494712103407757
3	21.92714453584019	18.143360752056402	27.920094007050526	32.00940070505288
4	17.2502937720329	14.500587544065805	31.351351351351354	36.89776733254994
5	17.344300822561692	32.52643948296122	31.515863689776737	18.613396004700352
6	36.827262044653345	32.00940070505288	14.35957696827262	16.803760282021152
7	31.045828437132784	26.415981198589893	18.683901292596943	23.854289071680377
8	28.061104582843715	29.471210340775556	19.200940070505286	23.266745005875443
9	28.860164512338425	11.562867215041129	16.145710928319623	43.431257344300825
10	20.305522914218564	21.856639247943598	29.28319623971798	28.554641598119858
11	41.36310223266745	18.143360752056402	17.156286721504113	23.337250293772033
12	23.948296122209165	21.90364277320799	25.82843713278496	28.319623971797885
13	36.051703877790835	16.051703877790835	18.871915393654522	29.024676850763807
14	25.875440658049353	18.777908343125734	21.292596944770857	34.054054054054056
15	29.870740305522915	24.488836662749705	18.51938895417156	27.121034077555816
16	28.860164512338425	21.974148061104586	20.634547591069328	28.53113983548766
17	27.473560517038777	23.29024676850764	21.433607520564042	27.80258519388954
18	29.306698002350178	19.71797884841363	20.752056404230316	30.223266745005876
19	28.860164512338425	21.62162162162162	21.55111633372503	27.967097532314924
20	31.30434782608696	20.517038777908343	21.410105757931845	26.768507638072858
21	29.494712103407757	20.117508813160985	20.65804935370153	29.72972972972973
22	28.46063454759107	21.034077555816687	20.987074030552293	29.518213866039954
23	29.424206815511162	21.292596944770857	21.128084606345475	28.155111633372503
24	29.400705052878966	20.44653349001175	20.517038777908343	29.63572267920094
25	27.873090481786132	21.997649823736783	22.326674500587544	27.80258519388954
26	28.90716803760282	21.339600470035254	21.574618096357227	28.1786133960047
27	26.90951821386604	20.82256169212691	23.83078730904818	28.437132784958873
28	27.82608695652174	21.48061104582844	22.115158636897768	28.578143360752055
29	28.484136310223267	21.504112808460636	21.715628672150412	28.296122209165688
30	29.048178613396004	20.82256169212691	21.292596944770857	28.83666274970623
31	28.64864864864865	20.681551116333726	21.997649823736783	28.672150411280846
32	28.789659224441834	20.869565217391305	21.128084606345475	29.212690951821386
33	28.1786133960047	20.094007050528788	23.47826086956522	28.24911868390129
34	28.34312573443008	20.72855464159812	22.068155111633374	28.860164512338425
35	26.321974148061106	21.26909518213866	25.875440658049353	26.53349001175088
36	27.614571092831962	19.741480611045827	23.54876615746181	29.095182138660398
37	27.19153936545241	20.282021151586367	23.877790834312574	28.64864864864865
38	24.159811985898944	21.45710928319624	25.311398354876612	29.0716803760282
39	26.486486486486488	19.506462984723854	25.687426556991777	28.319623971797885
40	26.50998824911868	18.848413631022325	26.439482961222094	28.202115158636897
41	23.83078730904818	20.0	27.003525264394828	29.165687426556993
42	22.115158636897768	19.482961222091657	30.17626321974148	28.225616921269097
43	22.7497062279671	20.18801410105758	27.84958871915394	29.212690951821386
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.0
10	1.5
11	2.0
12	2.0
13	2.0
14	2.0
15	2.0
16	2.0
17	5.5
18	9.0
19	9.0
20	8.0
21	7.0
22	5.0
23	3.0
24	5.0
25	7.0
26	7.0
27	7.0
28	7.0
29	8.5
30	10.0
31	13.5
32	17.0
33	17.0
34	24.5
35	32.0
36	32.5
37	33.0
38	45.0
39	57.0
40	57.0
41	73.5
42	90.0
43	119.0
44	148.0
45	197.0
46	246.0
47	246.0
48	286.0
49	326.0
50	374.0
51	422.0
52	432.0
53	442.0
54	442.0
55	439.0
56	436.0
57	416.5
58	397.0
59	376.0
60	355.0
61	355.0
62	352.5
63	350.0
64	307.5
65	265.0
66	228.5
67	192.0
68	192.0
69	164.0
70	136.0
71	116.0
72	96.0
73	79.0
74	62.0
75	62.0
76	53.0
77	44.0
78	32.5
79	21.0
80	18.5
81	16.0
82	16.0
83	14.5
84	13.0
85	8.5
86	4.0
87	3.0
88	2.0
89	2.0
90	1.5
91	1.0
92	1.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4255.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.34077555816687
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.69302809573361	85.54641598119859
2	3.2518210197710715	5.875440658049354
3	1.040582726326743	2.82021151586369
4	0.3902185223725286	1.410105757931845
5	0.2601456815816858	1.1750881316098707
6	0.07804370447450572	0.42303172737955347
7	0.0	0.0
8	0.18210197710718	1.3160987074030552
9	0.026014568158168577	0.21151586368977673
>10	0.07804370447450572	1.2220916568742655
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	28	0.6580493537015276	TruSeq Adapter, Index 12 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	13	0.30552291421856637	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	11	0.25851938895417154	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	9	0.21151586368977673	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	8	0.18801410105757932	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	8	0.18801410105757932	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	8	0.18801410105757932	No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC	8	0.18801410105757932	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	8	0.18801410105757932	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	8	0.18801410105757932	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	8	0.18801410105757932	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	6	0.1410105757931845	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	6	0.1410105757931845	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	6	0.1410105757931845	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	5	0.11750881316098707	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	5	0.11750881316098707	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	5	0.11750881316098707	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	5	0.11750881316098707	No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA	5	0.11750881316098707	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	5	0.11750881316098707	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	5	0.11750881316098707	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	5	0.11750881316098707	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	5	0.11750881316098707	No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC	5	0.11750881316098707	RNA PCR Primer, Index 12 (95% over 23bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.023501762632197415	0.0
28	0.0	0.0	0.0	0.1410105757931845	0.0
29	0.0	0.0	0.0	0.30552291421856637	0.0
30	0.0	0.0	0.0	0.6345475910693302	0.0
31	0.0	0.0	0.0	1.0105757931844888	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE