FastQCFastQC Report
Fri 10 Feb 2017
ERR1632419.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632419.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1666
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA110.6602641056422569No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA90.5402160864345739No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT90.5402160864345739RNA PCR Primer, Index 17 (95% over 21bp)
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA80.4801920768307323No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT60.36014405762304924No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA50.3001200480192077No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC50.3001200480192077No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC50.3001200480192077No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA50.3001200480192077No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA50.3001200480192077No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC50.3001200480192077No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC50.3001200480192077No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT50.3001200480192077No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC40.24009603841536614RNA PCR Primer, Index 17 (95% over 22bp)
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC40.24009603841536614No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT40.24009603841536614No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT40.24009603841536614No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA30.18007202881152462No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG30.18007202881152462No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC30.18007202881152462No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC30.18007202881152462No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA30.18007202881152462No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC30.18007202881152462No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA30.18007202881152462No Hit
GAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAA30.18007202881152462No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG30.18007202881152462No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT30.18007202881152462No Hit
TTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT30.18007202881152462No Hit
GGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTC30.18007202881152462No Hit
TGCGTGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACC30.18007202881152462No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA30.18007202881152462No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA30.18007202881152462No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT30.18007202881152462No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC30.18007202881152462No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG30.18007202881152462No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA30.18007202881152462No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC30.18007202881152462No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG20.12004801920768307No Hit
GGTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGGCCCC20.12004801920768307No Hit
ATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGAC20.12004801920768307No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACC20.12004801920768307No Hit
CAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAA20.12004801920768307No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20.12004801920768307No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACCTGTCTCTTA20.12004801920768307No Hit
TATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCAT20.12004801920768307No Hit
CATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACT20.12004801920768307No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCTGTCT20.12004801920768307No Hit
CGCTTGCAGCATGGCTGACCAACTGACTGAAGAGCAGCTGTCT20.12004801920768307No Hit
GCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACAATAAC20.12004801920768307No Hit
GTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGC20.12004801920768307No Hit
GGTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGAT20.12004801920768307No Hit
GACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCA20.12004801920768307No Hit
GTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGC20.12004801920768307No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC20.12004801920768307No Hit
TCAGCAGCCTTTCTGAACACATGCACGGCCACATTGATGGCAG20.12004801920768307No Hit
GTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGAC20.12004801920768307No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC20.12004801920768307No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG20.12004801920768307No Hit
TTAGTGGACCATGAAGTGCGATTGCCTCGGTGATAACGACATT20.12004801920768307No Hit
GTGTAGGATAGGTGGGAGGCTTTGAAGTGTGGACGCCAGTCTG20.12004801920768307No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC20.12004801920768307No Hit
CTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTT20.12004801920768307No Hit
GACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTG20.12004801920768307No Hit
GTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAA20.12004801920768307No Hit
ACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT20.12004801920768307No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT20.12004801920768307No Hit
GCATCAAACCTACCCCCCACGGTGTCCTGCTCTTGTCCAAGGA20.12004801920768307No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC20.12004801920768307No Hit
CTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCG20.12004801920768307No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC20.12004801920768307No Hit
TACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCT20.12004801920768307No Hit
ACGTCAATGAGCAAAGGTATTAACTTTACTCCCTTCCTCCCCG20.12004801920768307No Hit
GTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATG20.12004801920768307No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC20.12004801920768307No Hit
ATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAA20.12004801920768307No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT20.12004801920768307No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCTGTCTCTT20.12004801920768307No Hit
CTACTGGGGGAATCTCGGTTGATTTCTTTTCCTCGGGGTACTT20.12004801920768307No Hit
ATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGAATACCA20.12004801920768307No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC20.12004801920768307No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC20.12004801920768307No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA20.12004801920768307No Hit
ATCCAGATCATTCTCAGCTTCCCAGGCAGACCCACTCAGTGAT20.12004801920768307No Hit
GGACTAGGATGATGGCGGGCAGGATAGTTCAGACGCTGTCTCT20.12004801920768307No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG20.12004801920768307No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG20.12004801920768307No Hit
CGTTAGCACCCGCCGTGTGTCTCCCGTGATAACATTCTCCGGT20.12004801920768307No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT20.12004801920768307No Hit
GCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGT20.12004801920768307No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC20.12004801920768307No Hit
GCCATCAATGTGGCCGTGCATGTGTTCAGAAAGGCTGCTGATG20.12004801920768307No Hit
CTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTT20.12004801920768307No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATACTGTCTCT20.12004801920768307No Hit
CGTCAATGAGCAAAGGTATTAACTTTACTCCCTTCCTCCCCGC20.12004801920768307No Hit
CCCATCGACTACGCCTTTCGGCCTCGCCTTAGGGGTCGACTCA20.12004801920768307No Hit
GGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGC20.12004801920768307No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT20.12004801920768307No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA20.12004801920768307No Hit
CTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATC20.12004801920768307No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC20.12004801920768307No Hit
GTGATGACGAGGCACTACGGTGCTGAAGCAACAAATGCCCTGC20.12004801920768307No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC20.12004801920768307No Hit
CTTGAGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCC20.12004801920768307No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC20.12004801920768307No Hit
GTATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG20.12004801920768307No Hit
CAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGT20.12004801920768307No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA20.12004801920768307No Hit
GCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGA20.12004801920768307No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA20.12004801920768307No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA20.12004801920768307No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGTC20.12004801920768307No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGAT20.12004801920768307No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers