FastQCFastQC Report
Fri 10 Feb 2017
ERR1632418.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632418.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32209
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2070.6426775124965072No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1290.4005091744543451No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1220.3787761184762023No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT1200.37256667391101866TruSeq Adapter, Index 11 (95% over 23bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1140.3539383402154677No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1090.3384147288025086No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1090.3384147288025086No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA970.3011580614114068No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC950.2949486168462231No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA940.2918438945636313No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT840.26079667173771304No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT830.25769194945512125No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC800.24837778260734578No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT780.24216833804216212No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT760.23595889347697846No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT730.226644726629203No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG730.226644726629203No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT670.2080163929336521No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC650.20180694836846844No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC650.20180694836846844No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA630.1955975038032848No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA620.192492781520693No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC620.192492781520693No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC610.18938805923810115No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG600.18628333695550933No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA590.1831786146729175No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA580.1800738923903257No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA560.17386444782514204No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA540.1676550032599584No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC520.16144555869477475No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC520.16144555869477475No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT500.1552361141295911No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA500.1552361141295911No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC490.15213139184699928No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT470.14592194728181565No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC470.14592194728181565No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG460.1428172249992238No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC460.1428172249992238No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC460.1428172249992238No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC460.1428172249992238No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC460.1428172249992238No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA460.1428172249992238No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA450.139712502716632No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC450.139712502716632No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA440.13660778043404018No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC430.13350305815144836No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT430.13350305815144836No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA420.13039833586885652No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA420.13039833586885652No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA400.12418889130367289No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT400.12418889130367289No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT390.12108416902108106No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG390.12108416902108106No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG390.12108416902108106No Hit
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC390.12108416902108106TruSeq Adapter, Index 11 (95% over 21bp)
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG380.11797944673848923No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG380.11797944673848923No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT380.11797944673848923No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA370.11487472445589743No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA370.11487472445589743No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG370.11487472445589743No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA370.11487472445589743No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT360.1117700021733056No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG360.1117700021733056No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG360.1117700021733056No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC360.1117700021733056No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAA360.1117700021733056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT350.10866527989071377No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC350.10866527989071377No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC350.10866527989071377No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG350.10866527989071377No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA350.10866527989071377No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA350.10866527989071377No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT340.10556055760812194No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCG405.7654965E-527.75000218
TTCTTCT405.7654965E-527.75000215
TCTTCTG405.7654965E-527.75000216
TGCGGGT405.7654965E-527.75000221
CTTCTGC405.7654965E-527.75000217
GCGGGTA405.7654965E-527.75000222
GTAACGT358.6828007E-426.42857226
TCTGCGG451.2846208E-424.66666819
CTGCGGG451.2846208E-424.66666820
GGTATCA851.8007995E-923.9411771
GGGTAAC400.001890940923.12500224
TAGCCGG502.6239257E-422.25
GCGGTAA753.512996E-722.223
CGGGTAA502.6239257E-422.223
GCCGGTG502.6239257E-422.27
GCAGCCG753.512996E-722.217
CGCGGTA753.512996E-722.222
AGCCGGT502.6239257E-422.26
AGCCGCG753.512996E-722.219
TTAGCCG502.6239257E-422.24
Produced by FastQC (version 0.11.5)