##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632418.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32209
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.04070290912478	34.0	33.0	34.0	31.0	34.0
2	33.176192989537086	34.0	33.0	34.0	31.0	34.0
3	33.238442671303055	34.0	34.0	34.0	31.0	34.0
4	36.52274209071999	37.0	37.0	37.0	35.0	37.0
5	36.49035983731255	37.0	37.0	37.0	35.0	37.0
6	36.545779130056815	37.0	37.0	37.0	35.0	37.0
7	36.561923685926295	37.0	37.0	37.0	35.0	37.0
8	36.53345338259493	37.0	37.0	37.0	35.0	37.0
9	38.30898196156354	39.0	39.0	39.0	37.0	39.0
10	38.3660778043404	39.0	39.0	39.0	37.0	39.0
11	38.43273619174765	39.0	39.0	39.0	37.0	39.0
12	38.37427427116644	39.0	39.0	39.0	37.0	39.0
13	38.44068428079108	39.0	39.0	39.0	37.0	39.0
14	39.95985594088609	41.0	40.0	41.0	38.0	41.0
15	39.97180912167406	41.0	40.0	41.0	38.0	41.0
16	39.8975752118973	41.0	40.0	41.0	38.0	41.0
17	39.907603464870064	41.0	40.0	41.0	38.0	41.0
18	39.93082678754386	41.0	40.0	41.0	38.0	41.0
19	39.928467198609084	41.0	40.0	41.0	38.0	41.0
20	39.90415722313639	41.0	40.0	41.0	38.0	41.0
21	39.86016330839207	41.0	40.0	41.0	38.0	41.0
22	39.82110590207706	41.0	40.0	41.0	38.0	41.0
23	39.81573473252818	41.0	40.0	41.0	38.0	41.0
24	39.77363469837623	41.0	40.0	41.0	38.0	41.0
25	39.7464062839579	41.0	40.0	41.0	38.0	41.0
26	39.650687695985596	41.0	40.0	41.0	37.0	41.0
27	39.512620696078734	41.0	40.0	41.0	37.0	41.0
28	39.4706448508181	41.0	39.0	41.0	37.0	41.0
29	39.447669905926915	41.0	39.0	41.0	37.0	41.0
30	39.40519730510106	41.0	39.0	41.0	36.0	41.0
31	39.35443509578068	41.0	39.0	41.0	36.0	41.0
32	39.324816045204756	41.0	39.0	41.0	36.0	41.0
33	39.215002018069484	41.0	39.0	41.0	35.0	41.0
34	39.161787078145856	41.0	39.0	41.0	35.0	41.0
35	39.088732962836474	41.0	39.0	41.0	35.0	41.0
36	39.02188829209227	41.0	39.0	41.0	35.0	41.0
37	38.9323170542395	40.0	39.0	41.0	35.0	41.0
38	38.883200347728895	40.0	39.0	41.0	35.0	41.0
39	38.72898879195256	40.0	38.0	41.0	35.0	41.0
40	38.66295134900183	40.0	38.0	41.0	35.0	41.0
41	38.56639448601322	40.0	38.0	41.0	35.0	41.0
42	38.494427023502745	40.0	38.0	41.0	35.0	41.0
43	37.752305256294825	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	5.0
23	18.0
24	21.0
25	41.0
26	43.0
27	57.0
28	84.0
29	113.0
30	123.0
31	176.0
32	236.0
33	354.0
34	520.0
35	798.0
36	1251.0
37	2514.0
38	6493.0
39	19360.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.61231332857276	15.725418361327579	13.25405942438449	28.408208885715176
2	22.555807383029588	19.274115930330034	30.699493930267934	27.47058275637244
3	21.643019031947592	18.1098450743581	29.603526964513023	30.643608929181287
4	15.629171970567231	17.252941724362756	34.54314011611661	32.5747461889534
5	17.83973423577261	31.58433978080661	32.56853674438822	18.007389239032566
6	30.75848365363718	34.58350150579031	16.71272004719178	17.945294793380732
7	28.383371107454437	27.62271414821944	19.51007482380701	24.48383992051911
8	26.079667173771305	29.112980843863518	21.63370486509982	23.17364711726536
9	27.75621720637089	12.192244403738085	17.249837002080167	42.80170138781086
10	20.388711229780494	21.571610419447982	27.085597193331058	30.954081157440466
11	40.05091744543451	18.575553416746875	17.82421062435965	23.54931851345897
12	21.254928746623612	23.449967400416032	26.387034679747895	28.908069173212457
13	38.22223602098792	16.613368934148838	19.3237914868515	25.840603558011736
14	23.676612127045235	21.02517929771182	22.602378217268466	32.69583035797448
15	31.6247011704803	22.52165543792108	19.128193983048217	26.725449408550407
16	24.136111024868825	23.893942686826662	23.00288739172281	28.967058896581698
17	28.405104163432583	23.546213791176378	20.180694836846843	27.8679872085442
18	27.215995529199915	19.79570927380546	21.73926542270794	31.24902977428669
19	29.209227234623864	21.792045701512	22.537179049334036	26.461548014530102
20	32.60579341177932	18.71216119718091	21.24561457977584	27.436430811263936
21	28.305753050389644	20.907199850973328	20.944456518364433	29.842590580272592
22	29.79601974603372	21.838616535750877	19.91989816510913	28.445465553106274
23	29.93262752646776	20.56568039988823	20.74885901456115	28.752833059082867
24	28.259182216150762	20.354559284671986	22.8166040547673	28.56965444440995
25	29.22164612375423	21.695799310751653	21.9814337607501	27.101120804744017
26	29.075724176472416	22.490608215095158	21.695799310751653	26.737868297680773
27	26.809276910180387	21.326337359123226	25.381104660188146	26.48328107050824
28	26.141761619423143	23.217113229221646	20.68676456890931	29.9543605824459
29	27.886615542239745	24.06159769008662	20.85441957216927	27.197367195504363
30	27.563724424850193	20.67124095749635	24.151634636281784	27.613399981371668
31	29.327206681362348	21.431897916731348	21.832407091185694	27.408488310720607
32	26.505014126486387	20.941351796081843	22.22049737650967	30.333136700922104
33	26.82790524387594	20.363873451519762	24.794312148778292	28.01390915582601
34	26.6509360737682	21.335651525971002	23.797696296066317	28.215716104194478
35	25.508398273774414	23.487224067807137	24.862616038995313	26.141761619423143
36	27.234623862895464	21.158682355863267	24.800521593343476	26.806172187897793
37	27.67238970474091	20.959980129777392	23.723182961284113	27.644447204197586
38	25.967897171597997	19.624949548262908	26.144866341705736	28.262286938433355
39	25.322114936818902	18.389270079791363	27.09491136017883	29.193703623210904
40	24.90918687323419	20.0068303890217	28.597596944953274	26.486385792790834
41	22.856965444440995	19.603216492284766	28.65348194603993	28.88633611723431
42	20.51290012108417	20.339035673259026	31.64332950417585	27.504734701480952
43	19.26790648576485	21.764103200968673	29.52590890744823	29.44208140581825
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.0
6	1.0
7	0.0
8	2.5
9	5.0
10	5.0
11	5.0
12	5.0
13	9.0
14	13.0
15	32.5
16	52.0
17	47.0
18	42.0
19	42.0
20	54.5
21	67.0
22	41.5
23	16.0
24	20.5
25	25.0
26	25.0
27	21.0
28	17.0
29	22.5
30	28.0
31	24.5
32	21.0
33	21.0
34	47.5
35	74.0
36	74.0
37	74.0
38	153.0
39	232.0
40	232.0
41	376.5
42	521.0
43	749.0
44	977.0
45	1737.5
46	2498.0
47	2498.0
48	2888.5
49	3279.0
50	3786.5
51	4294.0
52	4378.0
53	4462.0
54	4462.0
55	3967.0
56	3472.0
57	3381.5
58	3291.0
59	3043.0
60	2795.0
61	2795.0
62	2551.5
63	2308.0
64	1782.5
65	1257.0
66	1066.5
67	876.0
68	876.0
69	745.0
70	614.0
71	512.5
72	411.0
73	316.5
74	222.0
75	222.0
76	182.5
77	143.0
78	100.5
79	58.0
80	45.0
81	32.0
82	32.0
83	25.0
84	18.0
85	11.0
86	4.0
87	3.0
88	2.0
89	2.0
90	1.5
91	1.0
92	0.5
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	32209.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.47629544537241
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.72376357056696	41.55360303020895
2	8.510253317249699	8.761526281474122
3	3.2448733413751505	5.011021764103201
4	1.9240048250904707	3.961625632587165
5	1.0735826296743065	2.763202831506722
6	0.8745476477683957	2.701108385854885
7	0.5609167671893848	2.021174205967276
8	0.38600723763570566	1.589617808687013
9	0.3558504221954162	1.6486075320562577
>10	2.1592279855247285	21.9814337607501
>50	0.14475271411338964	5.181781489645751
>100	0.04221954161640531	2.8252972771585583
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	207	0.6426775124965072	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	129	0.4005091744543451	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	122	0.3787761184762023	No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT	120	0.37256667391101866	TruSeq Adapter, Index 11 (95% over 23bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	114	0.3539383402154677	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	109	0.3384147288025086	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	109	0.3384147288025086	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	97	0.3011580614114068	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	95	0.2949486168462231	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	94	0.2918438945636313	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	84	0.26079667173771304	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	83	0.25769194945512125	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	80	0.24837778260734578	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	78	0.24216833804216212	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	76	0.23595889347697846	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	73	0.226644726629203	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	73	0.226644726629203	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	67	0.2080163929336521	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	65	0.20180694836846844	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	65	0.20180694836846844	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	63	0.1955975038032848	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	62	0.192492781520693	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	62	0.192492781520693	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	61	0.18938805923810115	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	60	0.18628333695550933	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	59	0.1831786146729175	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	58	0.1800738923903257	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	56	0.17386444782514204	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	54	0.1676550032599584	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	52	0.16144555869477475	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	52	0.16144555869477475	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	50	0.1552361141295911	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	50	0.1552361141295911	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	49	0.15213139184699928	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	47	0.14592194728181565	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	47	0.14592194728181565	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	46	0.1428172249992238	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	46	0.1428172249992238	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	46	0.1428172249992238	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	46	0.1428172249992238	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	46	0.1428172249992238	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	46	0.1428172249992238	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	45	0.139712502716632	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	45	0.139712502716632	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	44	0.13660778043404018	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	43	0.13350305815144836	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	43	0.13350305815144836	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	42	0.13039833586885652	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	42	0.13039833586885652	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	40	0.12418889130367289	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	40	0.12418889130367289	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	39	0.12108416902108106	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	39	0.12108416902108106	No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG	39	0.12108416902108106	No Hit
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC	39	0.12108416902108106	TruSeq Adapter, Index 11 (95% over 21bp)
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	38	0.11797944673848923	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	38	0.11797944673848923	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	38	0.11797944673848923	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	37	0.11487472445589743	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	37	0.11487472445589743	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	37	0.11487472445589743	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	37	0.11487472445589743	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	36	0.1117700021733056	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	36	0.1117700021733056	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	36	0.1117700021733056	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	36	0.1117700021733056	No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAA	36	0.1117700021733056	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	35	0.10866527989071377	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	35	0.10866527989071377	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	35	0.10866527989071377	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG	35	0.10866527989071377	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	35	0.10866527989071377	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	35	0.10866527989071377	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	34	0.10556055760812194	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.003104722282591822	0.0
21	0.0	0.0	0.0	0.006209444565183644	0.0
22	0.0	0.0	0.0	0.006209444565183644	0.0
23	0.0	0.0	0.0	0.009314166847775467	0.0
24	0.0	0.0	0.0	0.012418889130367289	0.0
25	0.0	0.0	0.0	0.015523611412959111	0.0
26	0.0	0.0	0.0	0.021733055978142755	0.0
27	0.0	0.0	0.0	0.05278027880406098	0.0
28	0.0	0.0	0.0	0.21422583749883573	0.0
29	0.0	0.0	0.0	0.4160327858673042	0.0
30	0.0	0.0	0.0	0.7792852929305474	0.0
31	0.0	0.0	0.0	1.5306280853177683	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTGCG	40	5.7654965E-5	27.750002	18
TTCTTCT	40	5.7654965E-5	27.750002	15
TCTTCTG	40	5.7654965E-5	27.750002	16
TGCGGGT	40	5.7654965E-5	27.750002	21
CTTCTGC	40	5.7654965E-5	27.750002	17
GCGGGTA	40	5.7654965E-5	27.750002	22
GTAACGT	35	8.6828007E-4	26.428572	26
TCTGCGG	45	1.2846208E-4	24.666668	19
CTGCGGG	45	1.2846208E-4	24.666668	20
GGTATCA	85	1.8007995E-9	23.941177	1
GGGTAAC	40	0.0018909409	23.125002	24
TAGCCGG	50	2.6239257E-4	22.2	5
GCGGTAA	75	3.512996E-7	22.2	23
CGGGTAA	50	2.6239257E-4	22.2	23
GCCGGTG	50	2.6239257E-4	22.2	7
GCAGCCG	75	3.512996E-7	22.2	17
CGCGGTA	75	3.512996E-7	22.2	22
AGCCGGT	50	2.6239257E-4	22.2	6
AGCCGCG	75	3.512996E-7	22.2	19
TTAGCCG	50	2.6239257E-4	22.2	4
>>END_MODULE