FastQCFastQC Report
Fri 10 Feb 2017
ERR1632417.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632417.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730175
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA38780.5311055568870476No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA29560.4048344574930667No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA24280.33252302530215355No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA23060.31581470195501077No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA22620.30978874927243466No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA20860.28568493854213034No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA20100.2752764748176807No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC20040.2744547539973294No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG19190.26281370904235285No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC17370.2378881774916972No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT17070.23377957338994076No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT16390.22446673742595952No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA16120.22076899373437872No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT15790.21624952922244667No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT15680.21474304105180264No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA15600.2136474132913343No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT15590.21351045982127573No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA15580.21337350635121716No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT15570.21323655288115864No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA15110.20693669325846545No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC15020.20570411202793848No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC14490.19844557811483549No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC14080.19283048584243503No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT13810.18913274215085424No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC13720.18790016092032732No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA13670.18721539357003458No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC13630.18666757968980038No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC13490.18475023110898073No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA12790.1751634882048824No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA12280.16817886123189646No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC12230.16749409388160372No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC12000.16434416407025712No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC11940.16352244324990584No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG11580.15859211832779813No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA11540.15804430444756395No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA11380.15585304892662719No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC11370.15571609545656864No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG11160.15284007258533913No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11150.1527031191152806No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG11060.15147053788475365No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC10940.14982709624405108No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA10920.149553189303934No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA10730.1469510733728216No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT10180.13941863251960146No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA10090.13818605128907455No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC9810.1343513541274352No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC9620.13174923819632278No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT9500.1301057965556202No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9470.12969493614544458No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC9280.1270928202143322No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9190.12586023898380527No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG9170.12558633204368816No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC9040.12380593693292705No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA8950.1225733557024001No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT8920.12216249529222448No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8870.12147772794193173No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC8760.11997123977128771No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC8750.11983428630122916No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA8740.1196973328311706No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC8720.11942342589105351No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA8690.11901256548087788No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT8600.11777998425035094No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG8570.1173691238401753No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC8560.11723217037011675No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG8270.11326051973841887No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA8160.11175403156777484No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC8030.10997363645701373No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA7940.1087410552264868No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC7850.10750847399595988No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT7780.10654979970555004No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7710.10559112541514021No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC7540.1032629164241449No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7490.10257814907385215No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT7490.10257814907385215No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG7330.10038689355291538No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTGGT303.598204E-430.8333328
CGTTATT502.7184797E-729.610
TAGGATC250.005495046329.631
TGTACTT250.005495046329.65
GGTATCA16000.029.0218751
AATGACT604.318281E-827.74999834
CACTGTG706.5847416E-926.428577
AGACCAT358.866286E-426.428576
ACTGTGC706.5847416E-926.428578
GCCTAGT706.5847416E-926.428571
ATACGGC2450.026.4285729
CTTTTTA1000.025.9000022
TATTGTT509.085157E-625.90000213
TTATTGT509.085157E-625.90000212
ATACACA1500.025.937
CAATGCT659.3819835E-825.61538533
CAAACAT951.0913936E-1125.3157912
AGAACAT951.0913936E-1125.315795
AACATTA951.0913936E-1125.3157914
AAACATT951.0913936E-1125.3157913
Produced by FastQC (version 0.11.5)