FastQCFastQC Report
Fri 10 Feb 2017
ERR1632416.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632416.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1898388
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA39520.20817662142828547No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA33540.17667621160690017No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA29990.15797613554236542No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA28830.15186568815226392No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC27420.14443833399705436No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA25840.13611548324157127No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA24520.1291622155218006No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG24270.12784530875669253No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA23560.12410529354378558No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT22740.11978583935423107No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC22680.11946978173060512No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT22070.11625652922374141No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA20010.10540521747925082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22350.029.6331121
ATACGGC3900.022.2948729
GCGGTAA17250.020.16231723
CTTATAC31150.019.59871737
GTATCAA34750.018.9525182
TAATACG17100.018.82456227
CGGTAAT18650.018.64879224
ATACACA5300.018.537
TACGGCT4750.018.30526430
GGTAATA19800.018.21969825
AACGTCA8300.017.83132628
TATACAC8550.017.74269137
GTAATAC19500.017.74102626
CGCGGTA20050.017.62344222
AGCCGCG20300.017.40640419
AATACGG18450.017.24661328
GTAACGT8950.017.15642526
AACTCCG20600.017.0631075
CGTGCCA20500.017.05609710
CATAGGG3500.016.9142862
Produced by FastQC (version 0.11.5)