##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632416.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1898388
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.07492409349406	34.0	33.0	34.0	31.0	34.0
2	33.21754193557903	34.0	33.0	34.0	31.0	34.0
3	33.29394728580248	34.0	34.0	34.0	31.0	34.0
4	36.5678149040133	37.0	37.0	37.0	35.0	37.0
5	36.535887289637316	37.0	37.0	37.0	35.0	37.0
6	36.57766325956548	37.0	37.0	37.0	35.0	37.0
7	36.57423192729832	37.0	37.0	37.0	35.0	37.0
8	36.54819668055213	37.0	37.0	37.0	35.0	37.0
9	38.3399984618529	39.0	39.0	39.0	37.0	39.0
10	38.380739343063695	39.0	39.0	39.0	37.0	39.0
11	38.44338775845612	39.0	39.0	39.0	37.0	39.0
12	38.40677511657259	39.0	39.0	39.0	37.0	39.0
13	38.43890974869205	39.0	39.0	39.0	37.0	39.0
14	40.009073487611595	41.0	40.0	41.0	38.0	41.0
15	40.00238992239732	41.0	40.0	41.0	38.0	41.0
16	39.97179607119303	41.0	40.0	41.0	38.0	41.0
17	39.96816772967381	41.0	40.0	41.0	38.0	41.0
18	39.97141206118033	41.0	40.0	41.0	38.0	41.0
19	39.97533644334035	41.0	40.0	41.0	38.0	41.0
20	39.94871490970234	41.0	40.0	41.0	38.0	41.0
21	39.91839023424084	41.0	40.0	41.0	38.0	41.0
22	39.88714793814542	41.0	40.0	41.0	38.0	41.0
23	39.85355944095728	41.0	40.0	41.0	38.0	41.0
24	39.836979584784565	41.0	40.0	41.0	38.0	41.0
25	39.79681392844877	41.0	40.0	41.0	38.0	41.0
26	39.72246347954159	41.0	40.0	41.0	38.0	41.0
27	39.647721119181114	41.0	40.0	41.0	37.0	41.0
28	39.608968240422925	41.0	40.0	41.0	37.0	41.0
29	39.570083670988225	41.0	40.0	41.0	37.0	41.0
30	39.51995903893198	41.0	40.0	41.0	37.0	41.0
31	39.46600958286715	41.0	40.0	41.0	37.0	41.0
32	39.42098770114434	41.0	39.0	41.0	36.0	41.0
33	39.361875970560284	41.0	39.0	41.0	36.0	41.0
34	39.322969277091936	41.0	39.0	41.0	36.0	41.0
35	39.24561628076031	41.0	39.0	41.0	35.0	41.0
36	39.201199649386744	41.0	39.0	41.0	35.0	41.0
37	39.15192257852452	41.0	39.0	41.0	35.0	41.0
38	39.08716658554521	41.0	39.0	41.0	35.0	41.0
39	39.004434815222176	41.0	39.0	41.0	35.0	41.0
40	38.933173302823235	41.0	39.0	41.0	35.0	41.0
41	38.88007193471514	40.0	39.0	41.0	35.0	41.0
42	38.81365189834744	40.0	38.0	41.0	35.0	41.0
43	38.10407250783296	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	1.0
12	0.0
13	0.0
14	1.0
15	3.0
16	3.0
17	8.0
18	13.0
19	38.0
20	90.0
21	202.0
22	315.0
23	579.0
24	1007.0
25	1489.0
26	2149.0
27	2959.0
28	4056.0
29	5701.0
30	7784.0
31	10133.0
32	13203.0
33	17933.0
34	27265.0
35	43561.0
36	69287.0
37	133484.0
38	346553.0
39	1210568.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.88290907864988	16.384427208768702	12.52304586838939	28.20961784419202
2	20.981274639325576	18.817596824253	32.488195247757574	27.712933288663855
3	20.94124067366629	19.428746915804357	28.69297530325729	30.937037107272065
4	15.514478599738304	15.638425864470276	33.59155241183572	35.25554312395569
5	17.23630785698182	32.577323497620085	33.21575989734448	16.97060874805361
6	34.79831309511017	33.462390196313926	15.201897609972251	16.537399098603657
7	30.699625155658378	27.404197666651918	19.76213503245912	22.134042145230584
8	27.40456640054615	30.81635577131756	19.863010090666396	21.916067737469895
9	27.976367317956075	12.893043993114158	17.7258811159784	41.40470757295137
10	19.357581274217917	23.259417990421348	29.619656255728543	27.763344479632195
11	39.5116804362438	19.347941516697322	18.656144054850746	22.484233992208125
12	23.094488587159212	23.61735324917772	26.460502278775465	26.827655884887598
13	34.77787470211569	17.61778940869833	20.645200032869994	26.959135856315992
14	24.116566265694892	19.511606689464955	22.93635442280503	33.435472622035114
15	29.465156754046063	23.79766412345632	20.103003179539694	26.63417594295792
16	25.878324136056484	23.824739726546944	22.42249740305986	27.87443873433671
17	26.890182618094933	23.43256489189776	21.886569025931475	27.790683464075837
18	26.8033194478684	21.606120561234057	23.192413774212646	28.398146216684893
19	28.247544759027132	22.207999629159055	22.966432573320102	26.578023038493708
20	29.549807520907212	21.019517611784313	22.632570370229903	26.798104497078572
21	27.537732012633874	22.21147626301894	21.97474910292311	28.276042621424068
22	28.497124929150413	22.864293284618316	21.314610079709734	27.323971706521533
23	28.356953373072315	22.178237536267613	22.251352199866414	27.213456890793665
24	27.906413230593536	21.79364808458545	22.344536522565463	27.955402162255556
25	28.08240465068258	21.88825466659081	22.867664565936995	27.161676116789614
26	27.70729692770919	22.720697770950935	22.49524333276443	27.07676196857544
27	27.03820293849308	21.944091513431395	23.809410931801086	27.208294616274436
28	26.535039201680583	22.87309022180924	22.352543315697318	28.239327260812857
29	27.541577380387995	23.39216219234424	22.456789655223275	26.609470772044492
30	27.05137200614416	21.87397939725704	23.675402499383686	27.399246097215112
31	27.619116850717557	22.27763765889797	22.79423384471457	27.309011645669905
32	25.640912184442797	22.20267932582802	23.115348390318523	29.04106009941066
33	25.976301999380524	21.724747522634992	24.582856613084363	27.716093864900117
34	26.46713948887161	21.355592218239895	24.501103041106454	27.676165251782038
35	25.73435988849487	22.695887247496298	24.827853947665073	26.74189891634376
36	26.036827034304892	21.353011080980284	25.44885450181944	27.161307382895384
37	25.942799891276174	20.91874790611824	25.439214744298848	27.69923745830673
38	25.135378015453107	20.175275022808826	26.670206512051276	28.019140449686788
39	24.54466631689623	19.39161014502831	27.904727589934197	28.158995948141264
40	24.18293836665634	20.245334462712574	28.874603084300997	26.69712408633009
41	22.668495586782047	19.612323718860424	29.75471821355803	27.9644624807995
42	21.052387604641414	20.477478787265827	30.69114427609108	27.778989332001675
43	20.461939287437552	20.735381808144595	30.341110457925357	28.461568446492496
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	14.0
2	17.0
3	51.0
4	85.0
5	85.0
6	97.5
7	110.0
8	112.5
9	115.0
10	206.0
11	297.0
12	297.0
13	565.0
14	833.0
15	1554.5
16	2276.0
17	2229.0
18	2182.0
19	2182.0
20	2592.0
21	3002.0
22	2631.5
23	2261.0
24	2691.5
25	3122.0
26	3122.0
27	3876.5
28	4631.0
29	6200.5
30	7770.0
31	10012.0
32	12254.0
33	12254.0
34	17355.0
35	22456.0
36	26123.0
37	29790.0
38	39062.0
39	48334.0
40	48334.0
41	57079.5
42	65825.0
43	77950.0
44	90075.0
45	114581.0
46	139087.0
47	139087.0
48	156435.5
49	173784.0
50	190588.5
51	207393.0
52	209811.5
53	212230.0
54	212230.0
55	197439.5
56	182649.0
57	172875.0
58	163101.0
59	154027.5
60	144954.0
61	144954.0
62	132622.0
63	120290.0
64	101412.5
65	82535.0
66	71930.5
67	61326.0
68	61326.0
69	52101.0
70	42876.0
71	36052.5
72	29229.0
73	23281.5
74	17334.0
75	17334.0
76	14255.5
77	11177.0
78	8919.0
79	6661.0
80	5331.0
81	4001.0
82	4001.0
83	3059.5
84	2118.0
85	1631.5
86	1145.0
87	883.5
88	622.0
89	622.0
90	457.0
91	292.0
92	193.5
93	95.0
94	64.5
95	34.0
96	34.0
97	20.5
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1898388.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.765729710262356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.91721563914946	40.56132769967677
2	8.018456159960767	7.660148192605484
3	2.334233161590056	3.3448905083172527
4	1.140172671752486	2.1784471864782775
5	0.6531191108126932	1.5598355457842996
6	0.4691712344792951	1.344618382438088
7	0.33224300907644194	1.1108880838768698
8	0.26118763201717304	0.9980654267676603
9	0.19359880838913923	0.8322649518370047
>10	1.3630675232464613	12.791313040404972
>50	0.16135375668999621	5.42097537132565
>100	0.13665907995203921	12.958023924258766
>500	0.01331059969362105	4.366556221230153
>1k	0.0062116131903564905	4.872645464998769
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	3952	0.20817662142828547	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	3354	0.17667621160690017	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2999	0.15797613554236542	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	2883	0.15186568815226392	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	2742	0.14443833399705436	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	2584	0.13611548324157127	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	2452	0.1291622155218006	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	2427	0.12784530875669253	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2356	0.12410529354378558	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	2274	0.11978583935423107	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	2268	0.11946978173060512	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	2207	0.11625652922374141	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	2001	0.10540521747925082	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0535254120864649E-4
2	0.0	0.0	0.0	0.0	1.0535254120864649E-4
3	0.0	0.0	0.0	0.0	1.0535254120864649E-4
4	0.0	0.0	0.0	5.2676270604323246E-5	1.0535254120864649E-4
5	5.2676270604323246E-5	0.0	0.0	5.2676270604323246E-5	1.0535254120864649E-4
6	1.0535254120864649E-4	0.0	0.0	5.2676270604323246E-5	1.0535254120864649E-4
7	1.0535254120864649E-4	0.0	0.0	5.2676270604323246E-5	1.0535254120864649E-4
8	3.6873389423026275E-4	0.0	0.0	5.2676270604323246E-5	1.0535254120864649E-4
9	3.6873389423026275E-4	0.0	0.0	5.2676270604323246E-5	1.0535254120864649E-4
10	4.2141016483458597E-4	0.0	0.0	5.2676270604323246E-5	1.0535254120864649E-4
11	4.2141016483458597E-4	0.0	0.0	5.2676270604323246E-5	1.0535254120864649E-4
12	4.2141016483458597E-4	0.0	0.0	5.2676270604323246E-5	1.0535254120864649E-4
13	4.2141016483458597E-4	0.0	0.0	5.2676270604323246E-5	1.0535254120864649E-4
14	4.2141016483458597E-4	0.0	0.0	1.0535254120864649E-4	1.0535254120864649E-4
15	4.2141016483458597E-4	0.0	0.0	1.5802881181296974E-4	1.0535254120864649E-4
16	4.7408643543890924E-4	0.0	0.0	7.901440590648487E-4	1.5802881181296974E-4
17	4.7408643543890924E-4	0.0	0.0	0.0014222593063167276	1.5802881181296974E-4
18	4.7408643543890924E-4	0.0	0.0	0.0020543745535686066	1.5802881181296974E-4
19	4.7408643543890924E-4	0.0	0.0	0.0024231084477988694	1.5802881181296974E-4
20	4.7408643543890924E-4	0.0	0.0	0.0035293101304896576	2.1070508241729298E-4
21	5.267627060432325E-4	0.0	0.0	0.005636360954662588	2.6338135302161626E-4
22	5.794389766475557E-4	0.0	0.0	0.008638908379109012	2.6338135302161626E-4
23	5.794389766475557E-4	5.2676270604323246E-5	0.0	0.010587930391468972	2.6338135302161626E-4
24	5.794389766475557E-4	5.2676270604323246E-5	0.0	0.012905686298059195	2.6338135302161626E-4
25	5.794389766475557E-4	5.2676270604323246E-5	0.0	0.01443329814558457	2.6338135302161626E-4
26	5.794389766475557E-4	5.2676270604323246E-5	0.0	0.01833134217030449	2.6338135302161626E-4
27	5.794389766475557E-4	5.2676270604323246E-5	0.0	0.04830414014416442	3.160576236259395E-4
28	5.794389766475557E-4	5.2676270604323246E-5	0.0	0.19558699275385222	3.6873389423026275E-4
29	6.32115247251879E-4	5.2676270604323246E-5	0.0	0.41319266662031157	3.6873389423026275E-4
30	7.374677884605255E-4	5.2676270604323246E-5	0.0	0.6895850584811957	3.6873389423026275E-4
31	7.374677884605255E-4	5.2676270604323246E-5	0.0	1.502169208823486	3.6873389423026275E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2235	0.0	29.633112	1
ATACGGC	390	0.0	22.29487	29
GCGGTAA	1725	0.0	20.162317	23
CTTATAC	3115	0.0	19.598717	37
GTATCAA	3475	0.0	18.952518	2
TAATACG	1710	0.0	18.824562	27
CGGTAAT	1865	0.0	18.648792	24
ATACACA	530	0.0	18.5	37
TACGGCT	475	0.0	18.305264	30
GGTAATA	1980	0.0	18.219698	25
AACGTCA	830	0.0	17.831326	28
TATACAC	855	0.0	17.742691	37
GTAATAC	1950	0.0	17.741026	26
CGCGGTA	2005	0.0	17.623442	22
AGCCGCG	2030	0.0	17.406404	19
AATACGG	1845	0.0	17.246613	28
GTAACGT	895	0.0	17.156425	26
AACTCCG	2060	0.0	17.063107	5
CGTGCCA	2050	0.0	17.056097	10
CATAGGG	350	0.0	16.914286	2
>>END_MODULE