FastQCFastQC Report
Fri 10 Feb 2017
ERR1632415.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632415.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181396
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4690.2585503539218064No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3480.19184546517012502No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2970.1637301814813998No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2730.1504994597455291No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2730.1504994597455291No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2680.1477430593838894No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2630.14498665902224966No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2550.14057641844362612No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2450.13506361772034664No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2220.12238417605680389No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2210.12183289598447596No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2180.1201790557674921No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1990.10970473439326114No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1880.10364065359765376No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1880.10364065359765376No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1870.1030893735253258No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1830.10088425323601402No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGACC303.5876405E-430.8333324
TAATACT250.005484295529.64
TGCGGTA250.005484295529.65
CTTGATT250.005484295529.615
GAAAACC250.005484295529.637
GGTGCGT250.005484295529.68
TAGGAGA250.005484295529.64
TTTTCCG250.005484295529.620
TACGGCT453.983834E-628.77777730
GGTATCA2200.027.751
ATCTAAG556.212995E-726.9090932
TTTCCGG358.840502E-426.4285721
TAAGTAC509.036083E-625.90000235
ATACGGC509.036083E-625.90000229
TCTAAGT659.3008566E-825.61538533
CTAAGTA551.8912282E-523.54545434
CATCTAA652.6622147E-622.7692331
GTAACGT652.6622147E-622.7692326
CTAATAC502.689961E-422.23
CGGTAAT1500.022.19999924
Produced by FastQC (version 0.11.5)