##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632415.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	181396
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.06483053650577	34.0	33.0	34.0	31.0	34.0
2	33.20719861518446	34.0	33.0	34.0	31.0	34.0
3	33.27992348232596	34.0	34.0	34.0	31.0	34.0
4	36.553209552581094	37.0	37.0	37.0	35.0	37.0
5	36.522442611744474	37.0	37.0	37.0	35.0	37.0
6	36.56650091512492	37.0	37.0	37.0	35.0	37.0
7	36.563672848353875	37.0	37.0	37.0	35.0	37.0
8	36.54019934287415	37.0	37.0	37.0	35.0	37.0
9	38.341755055238266	39.0	39.0	39.0	37.0	39.0
10	38.37404352907451	39.0	39.0	39.0	37.0	39.0
11	38.44121700588767	39.0	39.0	39.0	37.0	39.0
12	38.39979382125295	39.0	39.0	39.0	37.0	39.0
13	38.4306158900968	39.0	39.0	39.0	37.0	39.0
14	39.989178372180206	41.0	40.0	41.0	38.0	41.0
15	39.98518710445655	41.0	40.0	41.0	38.0	41.0
16	39.9507210743346	41.0	40.0	41.0	38.0	41.0
17	39.95207722331253	41.0	40.0	41.0	38.0	41.0
18	39.94792057156718	41.0	40.0	41.0	38.0	41.0
19	39.947832366755605	41.0	40.0	41.0	38.0	41.0
20	39.92526847339522	41.0	40.0	41.0	38.0	41.0
21	39.89352025402986	41.0	40.0	41.0	38.0	41.0
22	39.859390504752035	41.0	40.0	41.0	38.0	41.0
23	39.82754856777437	41.0	40.0	41.0	38.0	41.0
24	39.81208516174557	41.0	40.0	41.0	38.0	41.0
25	39.763925334627004	41.0	40.0	41.0	38.0	41.0
26	39.67374142759488	41.0	40.0	41.0	37.0	41.0
27	39.597091446338396	41.0	40.0	41.0	37.0	41.0
28	39.55184789080244	41.0	40.0	41.0	37.0	41.0
29	39.52193543407793	41.0	40.0	41.0	37.0	41.0
30	39.48372069946416	41.0	40.0	41.0	37.0	41.0
31	39.40752828066771	41.0	39.0	41.0	36.0	41.0
32	39.370972899071646	41.0	39.0	41.0	36.0	41.0
33	39.298948157622	41.0	39.0	41.0	36.0	41.0
34	39.250418972854966	41.0	39.0	41.0	35.0	41.0
35	39.18247921674127	41.0	39.0	41.0	35.0	41.0
36	39.13274824141657	41.0	39.0	41.0	35.0	41.0
37	39.073601402456504	41.0	39.0	41.0	35.0	41.0
38	39.002646144347175	41.0	39.0	41.0	35.0	41.0
39	38.89823921144898	41.0	39.0	41.0	35.0	41.0
40	38.82590024035811	40.0	38.0	41.0	35.0	41.0
41	38.77474144964608	40.0	38.0	41.0	35.0	41.0
42	38.69161392753975	40.0	38.0	41.0	35.0	41.0
43	37.96096385807846	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	1.0
19	6.0
20	7.0
21	21.0
22	30.0
23	53.0
24	107.0
25	148.0
26	212.0
27	301.0
28	419.0
29	556.0
30	794.0
31	1091.0
32	1398.0
33	1826.0
34	2688.0
35	4229.0
36	6742.0
37	13301.0
38	33914.0
39	113549.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.36424176938852	15.78866127147236	12.400493946944806	28.446603012194316
2	21.49826898057289	18.45575426139496	31.48801517122759	28.55796158680456
3	21.727601490661318	18.8471631127478	27.83027189133167	31.59496350525921
4	16.37577454850162	15.086330459326556	32.413614412666206	36.124280579505616
5	18.177909104941676	31.94998787183841	32.19530750402434	17.676795519195572
6	35.93574279476945	32.02055172109638	14.886767073143838	17.156938410990318
7	31.2278109770888	26.581622527508873	19.05885466052173	23.13171183488059
8	28.11197600829125	30.08776378751461	19.40505854594368	22.395201658250457
9	28.14174513219696	12.7081082273038	17.163002491785928	41.98714414871331
10	19.732518908906478	22.80314891177314	29.002844605173212	28.46148757414717
11	40.266599042977795	18.69390725264063	18.172396304218395	22.86709740016318
12	23.181327041390105	22.82575139473858	26.429469227546363	27.563452336324946
13	35.72680764735716	17.0499900769587	19.82899292156387	27.39420935412027
14	24.652142274361065	19.285982050320847	22.119010342014157	33.942865333303935
15	30.003417936448436	23.377031467066526	19.31630245429888	27.303248142186153
16	26.742596308628634	22.91781516681735	21.899049593155308	28.440538931398706
17	27.672605790645882	22.778341308518378	21.23034686542151	28.31870603541423
18	27.44933736135306	20.96738627092108	22.559483119804185	29.023793247921674
19	28.754217292553307	21.66751196277757	22.443714304615316	27.134556440053803
20	29.951597609649603	20.574323579351255	22.018126088778143	27.455952722221
21	28.14725793292024	21.52748682440627	21.46629473637787	28.858960506295617
22	28.92290899468566	22.29872764559307	20.95305298904055	27.82531037068072
23	28.99733180444993	21.496615140355907	21.514807382742728	27.99124567245143
24	28.490154137908224	21.319654237138636	21.728704050805973	28.46148757414717
25	29.007806125824164	21.518115063176698	22.132241063750026	27.341837747249116
26	28.53094886326049	22.07545921630025	21.899049593155308	27.494542327283956
27	27.622990584136364	21.255154468676267	23.490595161966084	27.631259785221285
28	26.994531301682507	22.385278616948554	21.396833447264548	29.22335663410439
29	28.04747623982888	22.878123001609737	21.758473174711682	27.315927583849696
30	27.757502921784383	21.19010342014157	23.028622461355265	28.023771196718783
31	28.297206112593443	21.913934155108162	21.94094687865223	27.847912853646168
32	26.508853557961586	21.742486052614172	22.313612207545923	29.435048181878322
33	26.85450616331121	21.415025689651372	23.535800127896977	28.194668019140444
34	26.911839290833317	21.116231890449626	23.350570023594788	28.621358795122276
35	26.527045800348407	22.244150918432602	23.990606187567533	27.238197093651458
36	26.84678824229862	20.81468169088624	24.46856601027586	27.86996405653929
37	26.970274978500075	20.446426602571172	24.574411784162827	28.00888663476593
38	26.128470308055306	19.804736598381442	25.750843458510666	28.31594963505259
39	25.48402390350394	19.078700743125538	26.944364815100663	28.49291053826986
40	25.042448565569252	20.042338309554786	27.826964210897703	27.08824891397826
41	23.55950517100708	19.24243092460694	28.626871595845554	28.571192308540432
42	21.953075040243444	20.188427528721693	29.880482480319305	27.97801495071556
43	21.37533352444376	20.740810161194293	28.910780833094446	28.9730754812675
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.5
2	3.0
3	7.0
4	11.0
5	11.0
6	16.0
7	21.0
8	15.5
9	10.0
10	22.5
11	35.0
12	35.0
13	56.5
14	78.0
15	131.0
16	184.0
17	206.0
18	228.0
19	228.0
20	241.5
21	255.0
22	216.0
23	177.0
24	217.5
25	258.0
26	258.0
27	297.5
28	337.0
29	436.0
30	535.0
31	719.5
32	904.0
33	904.0
34	1251.0
35	1598.0
36	1801.0
37	2004.0
38	2625.5
39	3247.0
40	3247.0
41	4002.0
42	4757.0
43	5723.0
44	6689.0
45	9194.5
46	11700.0
47	11700.0
48	13390.5
49	15081.0
50	16936.5
51	18792.0
52	19578.5
53	20365.0
54	20365.0
55	19428.5
56	18492.0
57	17941.5
58	17391.0
59	16523.0
60	15655.0
61	15655.0
62	14391.5
63	13128.0
64	11209.5
65	9291.0
66	8075.5
67	6860.0
68	6860.0
69	5915.5
70	4971.0
71	4148.5
72	3326.0
73	2717.5
74	2109.0
75	2109.0
76	1673.0
77	1237.0
78	991.5
79	746.0
80	604.0
81	462.0
82	462.0
83	346.5
84	231.0
85	180.5
86	130.0
87	93.5
88	57.0
89	57.0
90	42.5
91	28.0
92	17.0
93	6.0
94	5.0
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	181396.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.69707324344269
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.10008332128267	53.738202201831385
2	6.75654070571804	8.337175735859757
3	1.9836694361982394	3.6715979548770434
4	0.9877537360107084	2.437660583885473
5	0.609729753478365	1.8809270629530477
6	0.436027975932429	1.6140989980375882
7	0.29932496061105196	1.2927231812887472
8	0.2803664240933846	1.3838230241833331
9	0.17560302610267162	0.9750773486904037
>10	1.204277364071363	14.280202271588893
>50	0.10975187194681482	4.5780612137303685
>100	0.056871424554258594	5.810450423073948
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	469	0.2585503539218064	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	348	0.19184546517012502	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	297	0.1637301814813998	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	273	0.1504994597455291	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	273	0.1504994597455291	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	268	0.1477430593838894	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	263	0.14498665902224966	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	255	0.14057641844362612	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	245	0.13506361772034664	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	222	0.12238417605680389	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	221	0.12183289598447596	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	218	0.1201790557674921	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	199	0.10970473439326114	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	188	0.10364065359765376	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	188	0.10364065359765376	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	187	0.1030893735253258	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	183	0.10088425323601402	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	5.512800723279455E-4	0.0	0.0
11	0.0	0.0	5.512800723279455E-4	0.0	0.0
12	0.0	0.0	5.512800723279455E-4	0.0	0.0
13	0.0	0.0	5.512800723279455E-4	0.0	0.0
14	0.0	0.0	5.512800723279455E-4	0.0	0.0
15	0.0	0.0	5.512800723279455E-4	0.0	0.0
16	0.0	0.0	5.512800723279455E-4	5.512800723279455E-4	0.0
17	0.0	0.0	5.512800723279455E-4	0.001102560144655891	0.0
18	0.0	0.0	5.512800723279455E-4	0.001102560144655891	0.0
19	0.0	0.0	5.512800723279455E-4	0.0016538402169838365	0.0
20	0.0	0.0	5.512800723279455E-4	0.0016538402169838365	0.0
21	0.0	0.0	5.512800723279455E-4	0.002205120289311782	0.0
22	0.0	0.0	5.512800723279455E-4	0.004410240578623564	0.0
23	0.0	0.0	5.512800723279455E-4	0.0055128007232794545	0.0
24	0.0	0.0	5.512800723279455E-4	0.008269201084919183	0.0
25	0.0	0.0	5.512800723279455E-4	0.010474321374230964	0.0
26	0.0	0.0	5.512800723279455E-4	0.016538402169838366	0.0
27	0.0	0.0	5.512800723279455E-4	0.046307526075547424	0.0
28	0.0	0.0	5.512800723279455E-4	0.1708968224216631	0.0
29	0.0	0.0	5.512800723279455E-4	0.35447308650686893	0.0
30	0.0	0.0	5.512800723279455E-4	0.5821517563783104	0.0
31	0.0	0.0	5.512800723279455E-4	1.2778672076561777	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGACC	30	3.5876405E-4	30.833332	4
TAATACT	25	0.0054842955	29.6	4
TGCGGTA	25	0.0054842955	29.6	5
CTTGATT	25	0.0054842955	29.6	15
GAAAACC	25	0.0054842955	29.6	37
GGTGCGT	25	0.0054842955	29.6	8
TAGGAGA	25	0.0054842955	29.6	4
TTTTCCG	25	0.0054842955	29.6	20
TACGGCT	45	3.983834E-6	28.777777	30
GGTATCA	220	0.0	27.75	1
ATCTAAG	55	6.212995E-7	26.90909	32
TTTCCGG	35	8.840502E-4	26.42857	21
TAAGTAC	50	9.036083E-6	25.900002	35
ATACGGC	50	9.036083E-6	25.900002	29
TCTAAGT	65	9.3008566E-8	25.615385	33
CTAAGTA	55	1.8912282E-5	23.545454	34
CATCTAA	65	2.6622147E-6	22.76923	31
GTAACGT	65	2.6622147E-6	22.76923	26
CTAATAC	50	2.689961E-4	22.2	3
CGGTAAT	150	0.0	22.199999	24
>>END_MODULE