FastQCFastQC Report
Fri 10 Feb 2017
ERR1632412.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632412.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2351609
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA57040.242557329896254No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA45780.19467522024282097No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA40410.17183979139389244No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA36380.15470258873817883No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA35890.1526189090108092No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC34890.14836650140393237No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA32810.13952149358162858No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG31810.13526908597475173No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA29700.12629650592424166No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC28630.12174642978488345No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT28370.12064080380709548No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT27240.11583558321132469No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA25490.10839386989929024No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT24980.10622514201978304No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATAT303.6006139E-430.8333341
GGTATCA33000.029.8803041
ATACGGC3900.023.71794929
CTTATAC32550.020.68817137
TTATATA450.003826981420.5555552
GCGTATA450.003826981420.55555521
GTATCAA48600.020.2129632
GTTAGAC555.145293E-420.1818183
TACGGCT4850.019.83505230
ATACACA6000.019.73333437
GCGGTAA20950.019.51551223
ATAATAC500.00703701618.53
AATAGTC500.00703701618.55
GGTCTAG500.00703701618.51
GCAGTCG5400.018.1574069
TAGCGAA1950.018.02564210
CGCGGTA22750.017.8087922
CTAGTTC2300.017.6956523
GGTAATA23550.017.6751625
CGGTAAT23050.017.65726724
Produced by FastQC (version 0.11.5)