##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632412.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2351609
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.07802062332641	34.0	33.0	34.0	31.0	34.0
2	33.21053372393115	34.0	33.0	34.0	31.0	34.0
3	33.28254867199437	34.0	34.0	34.0	31.0	34.0
4	36.559020228277745	37.0	37.0	37.0	35.0	37.0
5	36.53233977247068	37.0	37.0	37.0	35.0	37.0
6	36.578549410212325	37.0	37.0	37.0	35.0	37.0
7	36.576997281435816	37.0	37.0	37.0	35.0	37.0
8	36.55010675669297	37.0	37.0	37.0	35.0	37.0
9	38.348862842419805	39.0	39.0	39.0	37.0	39.0
10	38.384540117000746	39.0	39.0	39.0	37.0	39.0
11	38.45108902032608	39.0	39.0	39.0	37.0	39.0
12	38.41371588559152	39.0	39.0	39.0	37.0	39.0
13	38.441116699247196	39.0	39.0	39.0	37.0	39.0
14	40.01091933225294	41.0	40.0	41.0	38.0	41.0
15	40.007214209505065	41.0	40.0	41.0	38.0	41.0
16	39.97306439973652	41.0	40.0	41.0	38.0	41.0
17	39.97297977682514	41.0	40.0	41.0	38.0	41.0
18	39.970130238487776	41.0	40.0	41.0	38.0	41.0
19	39.97875156966996	41.0	40.0	41.0	38.0	41.0
20	39.952643062686015	41.0	40.0	41.0	38.0	41.0
21	39.92214989821862	41.0	40.0	41.0	38.0	41.0
22	39.89341042664831	41.0	40.0	41.0	38.0	41.0
23	39.85523188591301	41.0	40.0	41.0	38.0	41.0
24	39.83631079826621	41.0	40.0	41.0	38.0	41.0
25	39.79148234251527	41.0	40.0	41.0	38.0	41.0
26	39.713431527094855	41.0	40.0	41.0	38.0	41.0
27	39.62635242508427	41.0	40.0	41.0	37.0	41.0
28	39.5843811620044	41.0	40.0	41.0	37.0	41.0
29	39.54585519956761	41.0	40.0	41.0	37.0	41.0
30	39.498903091457805	41.0	40.0	41.0	37.0	41.0
31	39.44001532567702	41.0	39.0	41.0	37.0	41.0
32	39.38533489198247	41.0	39.0	41.0	36.0	41.0
33	39.32051671855313	41.0	39.0	41.0	36.0	41.0
34	39.28274385750352	41.0	39.0	41.0	36.0	41.0
35	39.199932471767205	41.0	39.0	41.0	35.0	41.0
36	39.15525667744935	41.0	39.0	41.0	35.0	41.0
37	39.097928269538	41.0	39.0	41.0	35.0	41.0
38	39.024959081207804	41.0	39.0	41.0	35.0	41.0
39	38.93504022139735	41.0	39.0	41.0	35.0	41.0
40	38.86416151664669	40.0	39.0	41.0	35.0	41.0
41	38.80664812900444	40.0	38.0	41.0	35.0	41.0
42	38.73569628284294	40.0	38.0	41.0	35.0	41.0
43	38.010339729096124	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	3.0
12	1.0
13	0.0
14	1.0
15	9.0
16	4.0
17	11.0
18	42.0
19	91.0
20	120.0
21	241.0
22	455.0
23	855.0
24	1350.0
25	1920.0
26	2696.0
27	3738.0
28	5214.0
29	7184.0
30	9616.0
31	12610.0
32	16830.0
33	22741.0
34	34122.0
35	53772.0
36	86189.0
37	168309.0
38	440380.0
39	1483103.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.16725271930835	16.02617612026489	12.59184668879903	28.214724471627722
2	20.83377806429555	19.17882607185123	32.60886482404175	27.378531039811467
3	21.122048775965734	19.430100837341584	28.914415619263234	30.53343476742945
4	15.706310020075618	15.575676058392359	33.45173453580081	35.26627938573122
5	17.12857026827164	32.75187329186102	33.12264071110461	16.996915728762733
6	34.54217941843223	33.32365201868168	15.319128307469482	16.815040255416612
7	30.517148046295112	27.69388958793745	19.701361918584254	22.087600447183185
8	27.398985120400543	30.864867416309426	20.085396849561302	21.65075061372873
9	28.150002827851058	13.060079290392238	17.41335400570418	41.37656387605252
10	19.290791964140297	23.45866170779241	29.51698177715768	27.733564550909612
11	39.165184348248374	19.44017904336988	18.796024339080176	22.598612269301572
12	22.911929661776256	23.65325187988309	26.56912777591853	26.865690682422123
13	34.79455980990037	17.599524410733245	21.015143248728847	26.590772530637537
14	24.310674095906247	19.68358685478751	22.865025605872404	33.14071344343383
15	29.412670218560994	23.952833995787564	20.119458634492386	26.51503715115906
16	25.88249151963613	23.966611796433845	22.236689857880286	27.914206826049735
17	27.02894061045012	23.517217360539103	21.800903126327547	27.65293890268323
18	26.48042255323908	21.591174383156382	23.473630182568616	28.45477288103592
19	28.313465376259405	22.27245260585412	23.142452678145048	26.271629339741427
20	29.806655783338133	20.94285231941194	22.709727680069264	26.540764217180662
21	27.42088501957596	22.230523866850312	21.894881334439525	28.4537097791342
22	28.43878382843406	22.662823624165412	21.283470168722776	27.614922378677747
23	28.058916256911758	22.18719183333624	22.341639277618004	27.412252632134
24	27.94988452587144	21.746557357111662	22.573267919964586	27.730290197052316
25	28.22697140553553	22.041887065409256	22.900193016781277	26.830948512273938
26	27.962811844996338	22.633269391297617	22.38667227417483	27.01724648953121
27	27.226720088245965	21.73835021043039	23.84911777425584	27.18581192706781
28	26.50083410975209	22.88075951401785	22.32250344338706	28.295902932843003
29	27.2167269303698	23.476011530828465	22.248213882494923	27.059047656306813
30	26.978464532156494	22.427410339048713	23.521044527385293	27.073080601409504
31	27.74015578270027	22.339810742347048	22.726269545660013	27.19376392929267
32	25.51852795256354	22.26101362939162	23.221589983709027	28.99886843433581
33	26.161534506799388	21.771944230524717	24.44696375970665	27.619557502969244
34	26.518183932788148	21.626639462597737	24.12845842995158	27.72671817466254
35	25.91264108956889	22.761139288036404	24.577810341770252	26.748409280624458
36	26.15383764903094	21.5422716956773	25.249095406591827	27.054795248699932
37	26.59196320476746	21.092239398641528	24.86267912735493	27.453118269236082
38	25.557820198851083	20.53840583192189	26.207928273790415	27.695845695436617
39	24.939222464278714	19.69702446282524	27.354632509060817	28.00912056383523
40	24.569135430252224	20.45850309298867	28.338894773748528	26.633466703010576
41	22.935658096222628	20.03891803441814	29.225564283858414	27.79985958550082
42	21.70832821272584	20.696765491201983	29.865253960160892	27.729652335911286
43	20.94578648066069	21.13595414884022	29.58646611745405	28.33179325304504
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16.0
1	16.0
2	16.0
3	60.5
4	105.0
5	105.0
6	145.5
7	186.0
8	189.5
9	193.0
10	310.5
11	428.0
12	428.0
13	898.0
14	1368.0
15	2539.0
16	3710.0
17	3588.0
18	3466.0
19	3466.0
20	3969.0
21	4472.0
22	4153.0
23	3834.0
24	4440.5
25	5047.0
26	5047.0
27	5940.5
28	6834.0
29	9900.5
30	12967.0
31	15522.0
32	18077.0
33	18077.0
34	22923.0
35	27769.0
36	31296.5
37	34824.0
38	45652.5
39	56481.0
40	56481.0
41	67719.0
42	78957.0
43	89934.0
44	100911.0
45	133049.5
46	165188.0
47	165188.0
48	186423.0
49	207658.0
50	232775.5
51	257893.0
52	268089.0
53	278285.0
54	278285.0
55	254932.0
56	231579.0
57	217208.0
58	202837.0
59	189859.5
60	176882.0
61	176882.0
62	163932.5
63	150983.0
64	126121.5
65	101260.0
66	87141.5
67	73023.0
68	73023.0
69	62426.5
70	51830.0
71	44613.0
72	37396.0
73	30067.5
74	22739.0
75	22739.0
76	18505.0
77	14271.0
78	11570.5
79	8870.0
80	7015.0
81	5160.0
82	5160.0
83	4060.0
84	2960.0
85	2283.5
86	1607.0
87	1240.5
88	874.0
89	874.0
90	650.5
91	427.0
92	292.5
93	158.0
94	106.5
95	55.0
96	55.0
97	32.5
98	10.0
99	6.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2351609.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.65954391920869
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.61072976299259	34.83184868678479
2	8.637510635936124	7.196695073808263
3	2.512760282337869	3.1404134202149305
4	1.2214555970074916	2.0354113235558744
5	0.734010572772821	1.5289272846796427
6	0.5054073679763105	1.2633024265980464
7	0.34306325504879387	1.0004301118540342
8	0.26676746079934116	0.8890728599508748
9	0.2102746459634285	0.7883951263748558
>10	1.5164454264601035	12.709081666341614
>50	0.2158139442510993	6.31477156316439
>100	0.1986839450988617	16.317893084825506
>500	0.01846166137856274	5.333952605715309
>1k	0.008512877191226153	6.406084129587689
>5k	1.0256478543645968E-4	0.24372063654423937
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	5704	0.242557329896254	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	4578	0.19467522024282097	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	4041	0.17183979139389244	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	3638	0.15470258873817883	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	3589	0.1526189090108092	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	3489	0.14836650140393237	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	3281	0.13952149358162858	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	3181	0.13526908597475173	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2970	0.12629650592424166	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	2863	0.12174642978488345	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	2837	0.12064080380709548	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	2724	0.11583558321132469	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	2549	0.10839386989929024	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	2498	0.10622514201978304	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.2757222820630471E-4	0.0	0.0	0.0	0.0
7	1.2757222820630471E-4	0.0	0.0	0.0	0.0
8	2.1262038034384117E-4	0.0	0.0	4.2524076068768236E-5	0.0
9	2.1262038034384117E-4	0.0	0.0	4.2524076068768236E-5	0.0
10	2.5514445641260943E-4	0.0	0.0	4.2524076068768236E-5	0.0
11	2.5514445641260943E-4	0.0	0.0	4.2524076068768236E-5	0.0
12	2.5514445641260943E-4	0.0	0.0	8.504815213753647E-5	0.0
13	2.5514445641260943E-4	0.0	0.0	8.504815213753647E-5	0.0
14	2.9766853248137763E-4	0.0	0.0	1.7009630427507294E-4	0.0
15	3.827166846189141E-4	0.0	0.0	5.528129888939871E-4	0.0
16	3.827166846189141E-4	0.0	0.0	9.355296735129012E-4	0.0
17	3.827166846189141E-4	0.0	0.0	0.0015733908145444248	0.0
18	3.827166846189141E-4	0.0	0.0	0.0020411556513008754	0.0
19	3.827166846189141E-4	0.0	0.0	0.0024238723359197895	0.0
20	3.827166846189141E-4	0.0	0.0	0.003231829781226386	0.0
21	3.827166846189141E-4	0.0	0.0	0.005953370649627553	0.0
22	3.827166846189141E-4	0.0	0.0	0.009185200430853939	0.0
23	3.827166846189141E-4	4.2524076068768236E-5	0.0	0.011396452386429888	0.0
24	3.827166846189141E-4	4.2524076068768236E-5	0.0	0.014373137711243664	0.0
25	3.827166846189141E-4	4.2524076068768236E-5	0.0	0.016031576677925624	0.0
26	3.827166846189141E-4	4.2524076068768236E-5	0.0	0.020666700969421362	0.0
27	4.2524076068768235E-4	4.2524076068768236E-5	0.0	0.04626619476281984	0.0
28	4.677648367564506E-4	8.504815213753647E-5	0.0	0.16414293362544538	0.0
29	5.102889128252189E-4	8.504815213753647E-5	0.0	0.34759179778611154	0.0
30	5.102889128252189E-4	8.504815213753647E-5	0.0	0.5908720369755346	0.0
31	5.102889128252189E-4	8.504815213753647E-5	0.0	1.3352559885593225	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAATAT	30	3.6006139E-4	30.833334	1
GGTATCA	3300	0.0	29.880304	1
ATACGGC	390	0.0	23.717949	29
CTTATAC	3255	0.0	20.688171	37
TTATATA	45	0.0038269814	20.555555	2
GCGTATA	45	0.0038269814	20.555555	21
GTATCAA	4860	0.0	20.212963	2
GTTAGAC	55	5.145293E-4	20.181818	3
TACGGCT	485	0.0	19.835052	30
ATACACA	600	0.0	19.733334	37
GCGGTAA	2095	0.0	19.515512	23
ATAATAC	50	0.007037016	18.5	3
AATAGTC	50	0.007037016	18.5	5
GGTCTAG	50	0.007037016	18.5	1
GCAGTCG	540	0.0	18.157406	9
TAGCGAA	195	0.0	18.025642	10
CGCGGTA	2275	0.0	17.80879	22
CTAGTTC	230	0.0	17.695652	3
GGTAATA	2355	0.0	17.67516	25
CGGTAAT	2305	0.0	17.657267	24
>>END_MODULE