FastQCFastQC Report
Fri 10 Feb 2017
ERR1632411.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632411.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74956
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4450.5936816265542452No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2890.3855595282565772No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2630.35087251187363255No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2580.3442019317999893No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2200.293505523240301No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2190.29217140722557233No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2140.28550082715192915No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1950.26015262287208496No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1860.2481455787395272No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1860.2481455787395272No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT1840.24547734671006993Illumina PCR Primer Index 10 (95% over 21bp)
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1820.24280911468061261No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1780.2374726506216981No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1720.2294679545333262No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1650.22012914243022572No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1630.21746091040076845No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1530.20411975025348203No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1520.2027856342387534No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1520.2027856342387534No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1510.20145151822402477No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1460.19478093815038156No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1440.19211270612092426No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1400.1867762420620097No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1380.18410801003255242No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1340.17877154597363787No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1320.1761033139441806No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1310.17476919792945195No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1260.16809861785580874No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1250.1667645018410801No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1240.16543038582635144No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1210.16142803778216552No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1200.1600939217674369No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1170.15609157372325097No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1170.15609157372325097No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1160.15475745770852234No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1160.15475745770852234No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1160.15475745770852234No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG1120.1494209936496078No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1090.14541864560542184No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1090.14541864560542184No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1070.14275041357596457No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1060.14141629756123592No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1050.14008218154650728No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC1040.13874806553177862No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1000.13341160147286407No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA980.1307433694434068No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG970.12940925342867815No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG940.12540690538449226No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC930.1240727893697636No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG900.12007044132557768No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC900.12007044132557768No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG880.11740220929612039No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC880.11740220929612039No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT860.1147339772666631No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG850.11339986125193446No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA850.11339986125193446No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC850.11339986125193446No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT840.11206574523720583No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA830.11073162922247719No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC820.10939751320774856No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC800.10672928117829127No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA790.10539516516356263No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT790.10539516516356263No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT780.10406104914883398No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT780.10406104914883398No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG770.10272693313410534No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA760.1013928171193767No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT760.1013928171193767No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT750.10005870110464807No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTCAT303.5677495E-430.83333434
GGTATCA1500.030.8333341
TATAACG358.791949E-426.42857228
GTTCATA358.791949E-426.42857235
GGTTATA358.791949E-426.42857225
AACGGTT358.791949E-426.42857231
CGGTTCA358.791949E-426.42857233
CGCGGTA2400.026.20833422
ATACGGC508.943969E-625.89999829
GCGGTAA2450.025.67346823
CCGCGGT2450.025.67346821
ACGGAGC809.422365E-1025.43750231
GCAGCCG2450.024.91836717
CCAGCAG2550.024.66666614
GAGCTGT901.364242E-1024.66666634
GGTAATA2550.024.66666625
CAGCCGC2550.024.66666618
GCCAGCA2600.024.19230813
CGGTAAT2600.024.19230824
AGCCGCG2650.023.7358519
Produced by FastQC (version 0.11.5)