##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1632411.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 74956 Sequences flagged as poor quality 0 Sequence length 43 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.00328192539623 34.0 33.0 34.0 31.0 34.0 2 33.14455147019585 34.0 33.0 34.0 31.0 34.0 3 33.213645338598646 34.0 33.0 34.0 31.0 34.0 4 36.50362879556006 37.0 37.0 37.0 35.0 37.0 5 36.48146912855542 37.0 37.0 37.0 35.0 37.0 6 36.51982496397887 37.0 37.0 37.0 35.0 37.0 7 36.53647473184268 37.0 37.0 37.0 35.0 37.0 8 36.50416244196595 37.0 37.0 37.0 35.0 37.0 9 38.301710336730885 39.0 39.0 39.0 37.0 39.0 10 38.343574897273065 39.0 39.0 39.0 37.0 39.0 11 38.3925636373339 39.0 39.0 39.0 37.0 39.0 12 38.34472223704574 39.0 39.0 39.0 37.0 39.0 13 38.390055499226214 39.0 39.0 39.0 37.0 39.0 14 39.89583222156999 41.0 40.0 41.0 38.0 41.0 15 39.91393617588986 41.0 40.0 41.0 38.0 41.0 16 39.853674155504564 41.0 40.0 41.0 38.0 41.0 17 39.86554778803565 41.0 40.0 41.0 38.0 41.0 18 39.89025561662842 41.0 40.0 41.0 38.0 41.0 19 39.878955653983674 41.0 40.0 41.0 38.0 41.0 20 39.8620924275575 41.0 40.0 41.0 38.0 41.0 21 39.81386413362506 41.0 40.0 41.0 38.0 41.0 22 39.77522813383852 41.0 40.0 41.0 38.0 41.0 23 39.732029457281605 41.0 40.0 41.0 38.0 41.0 24 39.7082154864187 41.0 40.0 41.0 38.0 41.0 25 39.66260205987513 41.0 40.0 41.0 37.0 41.0 26 39.56502481455787 41.0 40.0 41.0 37.0 41.0 27 39.41748492448903 41.0 39.0 41.0 37.0 41.0 28 39.40539783339559 41.0 39.0 41.0 37.0 41.0 29 39.369216607076154 41.0 39.0 41.0 36.0 41.0 30 39.3281791984631 41.0 39.0 41.0 36.0 41.0 31 39.26584929825498 41.0 39.0 41.0 36.0 41.0 32 39.18665617162068 41.0 39.0 41.0 35.0 41.0 33 39.105221730081645 40.0 39.0 41.0 35.0 41.0 34 39.080100325524306 40.0 39.0 41.0 35.0 41.0 35 38.97900101392817 40.0 39.0 41.0 35.0 41.0 36 38.9061049148834 40.0 39.0 41.0 35.0 41.0 37 38.83107423021506 40.0 38.0 41.0 35.0 41.0 38 38.744383371577996 40.0 38.0 41.0 35.0 41.0 39 38.62016382944661 40.0 38.0 41.0 35.0 41.0 40 38.55160360744971 40.0 38.0 41.0 35.0 41.0 41 38.48483110091254 40.0 37.0 41.0 35.0 41.0 42 38.41317572976146 40.0 37.0 41.0 35.0 41.0 43 37.680719355355144 39.0 36.0 41.0 33.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 1.0 20 4.0 21 10.0 22 17.0 23 31.0 24 47.0 25 70.0 26 106.0 27 122.0 28 172.0 29 262.0 30 324.0 31 463.0 32 598.0 33 844.0 34 1201.0 35 2095.0 36 3166.0 37 6349.0 38 16266.0 39 42807.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.09995197182347 15.605155024280911 13.016969955707347 28.27792304818827 2 23.18426810395432 19.303324617108704 30.19905010939751 27.313357169539465 3 22.120977640215596 18.418805699343615 28.83958589038903 30.620630770051765 4 15.554458615721224 16.527029190458403 35.15662522012914 32.76188697369123 5 18.253375313517264 31.781311702865683 31.825337531351728 18.13997545226533 6 31.361065158226157 34.31079566679118 16.457655157692514 17.870484017290146 7 28.85025881850686 26.761033139441803 19.117882491061422 25.270825550989912 8 26.49287582048135 28.863599978654143 21.371204439938097 23.27231976092641 9 27.468114627247985 11.902983083408932 17.164736645498692 43.46416564384439 10 20.98297667965206 21.335183307540422 26.72234377501467 30.959496237792838 11 41.072095629435935 18.724318266716473 17.615667858476975 22.587918245370616 12 21.75009338812103 23.08287528683494 26.27674902609531 28.890282298948716 13 38.343828379315866 16.404290517103366 18.86440044826298 26.387480655317784 14 24.07679171780778 20.31591867228774 22.46784780404504 33.139441805859434 15 31.63589305726026 22.586584129355888 18.614920753508727 27.16260205987513 16 24.6971556646566 23.373712578045787 22.786701531565186 29.14243022573243 17 27.969742248785955 22.758685095255885 20.211857623138908 29.059715032819255 18 27.312023053524737 19.41672447836064 21.872832061476068 31.39842040663856 19 29.230481882704517 21.62468648273654 21.778109824430334 27.36672181012861 20 32.34697689311062 18.584236085169966 20.89492502267997 28.173861999039435 21 28.659480228400664 20.285234003948982 20.84556273013501 30.20972303751534 22 29.21047014248359 21.804792144724907 19.462084422861412 29.52265328993009 23 30.429852179945566 20.17983883878542 20.71348524467688 28.676823736592134 24 28.514061582795243 19.442072682640482 21.914189657932653 30.129676076631622 25 30.312449970649446 20.921607342974546 21.743422808047388 27.022519878328623 26 29.686749559741717 22.048935375420246 21.185762313890816 27.07855275094722 27 27.754949570414645 20.696141736485405 24.506377074550404 27.042531618549546 28 26.819734244089865 23.214952772293078 20.492021986231922 29.47329099738513 29 28.147179678744866 23.668552217300817 20.43732322962805 27.746944874326275 30 28.063130369816957 20.718821708735792 23.76460857036128 27.453439351085972 31 29.48930038956188 21.1710870377288 21.579326538235765 27.76028603447356 32 26.27541491008058 20.510699610438124 22.283739794012487 30.93014568546881 33 26.654303858263518 20.866908586370673 23.994076524894606 28.48471103047121 34 26.554245157158867 21.152409413522598 23.28165857302951 29.01168685628902 35 26.019264635252682 23.170926943807032 24.319600832488394 26.49020758845189 36 27.191952612199156 21.03500720422648 25.01734350819147 26.75569667538289 37 27.661561449383637 20.618763007631145 23.90869309995197 27.810982443033243 38 26.01392817119377 19.52478787555366 25.824483697102302 28.636800256150273 39 25.353540743903093 18.566892576978493 27.137253855595283 28.94231282352313 40 25.226799722503866 19.688884145365282 28.56208975932547 26.52222637280538 41 23.00683067399541 18.860398100218795 28.99567746411228 29.137093761673516 42 20.90826618282726 19.703559421527295 31.246331180959498 28.141843214685945 43 19.7409146699397 21.056353060462136 29.641389615240943 29.561342654357226 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 1.0 4 2.0 5 2.0 6 2.0 7 2.0 8 3.5 9 5.0 10 7.0 11 9.0 12 9.0 13 22.5 14 36.0 15 52.5 16 69.0 17 68.0 18 67.0 19 67.0 20 79.5 21 92.0 22 65.0 23 38.0 24 34.0 25 30.0 26 30.0 27 28.5 28 27.0 29 25.5 30 24.0 31 29.5 32 35.0 33 35.0 34 112.5 35 190.0 36 166.0 37 142.0 38 307.0 39 472.0 40 472.0 41 780.0 42 1088.0 43 1617.5 44 2147.0 45 3861.5 46 5576.0 47 5576.0 48 6606.5 49 7637.0 50 8490.0 51 9343.0 52 9537.0 53 9731.0 54 9731.0 55 9031.5 56 8332.0 57 7991.0 58 7650.0 59 7112.5 60 6575.0 61 6575.0 62 6078.0 63 5581.0 64 4451.5 65 3322.0 66 2898.5 67 2475.0 68 2475.0 69 2102.0 70 1729.0 71 1426.0 72 1123.0 73 886.0 74 649.0 75 649.0 76 533.5 77 418.0 78 314.5 79 211.0 80 141.0 81 71.0 82 71.0 83 55.5 84 40.0 85 25.5 86 11.0 87 7.5 88 4.0 89 4.0 90 2.0 91 0.0 92 1.0 93 2.0 94 1.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 74956.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.54538662682106 #Duplication Level Percentage of deduplicated Percentage of total 1 82.14066024195202 37.41128128502055 2 7.349365827938721 6.69459416190832 3 2.8091039573508305 3.8382517743742994 4 1.646211078238964 2.9990928011099847 5 1.075016842906939 2.448102887027056 6 0.7528047101555406 2.0572068947115643 7 0.5858402413661794 1.867762420620097 8 0.4364509798178037 1.5902662895565398 9 0.35736254723336947 1.4648593841720476 >10 2.4165909956354903 21.117722397139655 >50 0.3017077243035824 9.116014728640803 >100 0.12888485310055947 9.394844975719089 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA 445 0.5936816265542452 No Hit GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA 289 0.3855595282565772 No Hit CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA 263 0.35087251187363255 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA 258 0.3442019317999893 No Hit GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT 220 0.293505523240301 No Hit GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC 219 0.29217140722557233 No Hit CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT 214 0.28550082715192915 No Hit ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA 195 0.26015262287208496 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA 186 0.2481455787395272 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT 186 0.2481455787395272 No Hit TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT 184 0.24547734671006993 Illumina PCR Primer Index 10 (95% over 21bp) CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA 182 0.24280911468061261 No Hit CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC 178 0.2374726506216981 No Hit CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC 172 0.2294679545333262 No Hit TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA 165 0.22012914243022572 No Hit CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT 163 0.21746091040076845 No Hit CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA 153 0.20411975025348203 No Hit CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC 152 0.2027856342387534 No Hit ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA 152 0.2027856342387534 No Hit GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC 151 0.20145151822402477 No Hit GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC 146 0.19478093815038156 No Hit GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC 144 0.19211270612092426 No Hit AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA 140 0.1867762420620097 No Hit GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT 138 0.18410801003255242 No Hit GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA 134 0.17877154597363787 No Hit CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC 132 0.1761033139441806 No Hit CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT 131 0.17476919792945195 No Hit GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA 126 0.16809861785580874 No Hit GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG 125 0.1667645018410801 No Hit GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC 124 0.16543038582635144 No Hit TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC 121 0.16142803778216552 No Hit CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA 120 0.1600939217674369 No Hit ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG 117 0.15609157372325097 No Hit GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC 117 0.15609157372325097 No Hit GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC 116 0.15475745770852234 No Hit TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT 116 0.15475745770852234 No Hit GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC 116 0.15475745770852234 No Hit CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG 112 0.1494209936496078 No Hit GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA 109 0.14541864560542184 No Hit GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC 109 0.14541864560542184 No Hit CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA 107 0.14275041357596457 No Hit GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC 106 0.14141629756123592 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA 105 0.14008218154650728 No Hit GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC 104 0.13874806553177862 No Hit AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA 100 0.13341160147286407 No Hit GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA 98 0.1307433694434068 No Hit GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG 97 0.12940925342867815 No Hit GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG 94 0.12540690538449226 No Hit CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC 93 0.1240727893697636 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG 90 0.12007044132557768 No Hit AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC 90 0.12007044132557768 No Hit GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG 88 0.11740220929612039 No Hit TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC 88 0.11740220929612039 No Hit CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT 86 0.1147339772666631 No Hit AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG 85 0.11339986125193446 No Hit ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA 85 0.11339986125193446 No Hit GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC 85 0.11339986125193446 No Hit GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT 84 0.11206574523720583 No Hit CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA 83 0.11073162922247719 No Hit GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC 82 0.10939751320774856 No Hit CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC 80 0.10672928117829127 No Hit GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA 79 0.10539516516356263 No Hit GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT 79 0.10539516516356263 No Hit GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT 78 0.10406104914883398 No Hit GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT 78 0.10406104914883398 No Hit ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG 77 0.10272693313410534 No Hit CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA 76 0.1013928171193767 No Hit TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT 76 0.1013928171193767 No Hit ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT 75 0.10005870110464807 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0013341160147286408 0.0 16 0.0 0.0 0.0 0.0013341160147286408 0.0 17 0.0 0.0 0.0 0.0013341160147286408 0.0 18 0.0 0.0 0.0 0.0013341160147286408 0.0 19 0.0 0.0 0.0 0.0013341160147286408 0.0 20 0.0 0.0 0.0 0.0013341160147286408 0.0 21 0.0 0.0 0.0 0.0013341160147286408 0.0 22 0.0 0.0 0.0 0.0040023480441859225 0.0 23 0.0 0.0 0.0 0.005336464058914563 0.0 24 0.0 0.0 0.0 0.006670580073643204 0.0 25 0.0 0.0 0.0 0.009338812103100485 0.0 26 0.0 0.0 0.0 0.021345856235658253 0.0 27 0.0 0.0 0.0 0.06003522066278884 0.0 28 0.0 0.0 0.0 0.2481455787395272 0.0 29 0.0 0.0 0.0 0.49228880943486847 0.0 30 0.0 0.0 0.0 0.857836597470516 0.0 31 0.0 0.0 0.0 1.5689204333208815 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTTCAT 30 3.5677495E-4 30.833334 34 GGTATCA 150 0.0 30.833334 1 TATAACG 35 8.791949E-4 26.428572 28 GTTCATA 35 8.791949E-4 26.428572 35 GGTTATA 35 8.791949E-4 26.428572 25 AACGGTT 35 8.791949E-4 26.428572 31 CGGTTCA 35 8.791949E-4 26.428572 33 CGCGGTA 240 0.0 26.208334 22 ATACGGC 50 8.943969E-6 25.899998 29 GCGGTAA 245 0.0 25.673468 23 CCGCGGT 245 0.0 25.673468 21 ACGGAGC 80 9.422365E-10 25.437502 31 GCAGCCG 245 0.0 24.918367 17 CCAGCAG 255 0.0 24.666666 14 GAGCTGT 90 1.364242E-10 24.666666 34 GGTAATA 255 0.0 24.666666 25 CAGCCGC 255 0.0 24.666666 18 GCCAGCA 260 0.0 24.192308 13 CGGTAAT 260 0.0 24.192308 24 AGCCGCG 265 0.0 23.73585 19 >>END_MODULE