FastQCFastQC Report
Fri 10 Feb 2017
ERR1632410.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632410.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427513
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9430.2205780876838833No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6970.16303597785330504No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6800.15905949058859029No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5890.13777358817158775No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5880.1375396771560163No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5160.12069808403487145No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5150.1204641730193No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4730.11063991036529884No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4720.11040599934972738No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4560.10666342310058406No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4510.1054938680227268No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4340.10151738075801205No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT4290.10034782568015477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4750.029.61
AAGAGCG358.860249E-426.428577
GCTATAC358.860249E-426.428573
ATAGATT358.860249E-426.4285712
CCGTTAC358.860249E-426.4285714
CGTTAGA451.3213136E-424.6666661
TAGAACA502.6982572E-422.24
CTAAATC502.6982572E-422.23
GCGGTAA4150.021.84337423
ATACGGC855.1615643E-821.76470629
ATACACA1151.3460522E-1020.91304237
GAATATA450.00382193720.5555551
TAGATTG450.00382193720.55555513
GATTGGT450.00382193720.55555515
GTCCAAT450.00382193720.55555520
TAATACG4250.020.45882427
AGATTGG555.135833E-420.18181814
CGGTAAT4500.020.14444424
TAAACTA656.8891764E-519.9230775
CTTATAC5450.019.68807437
Produced by FastQC (version 0.11.5)