##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632410.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	427513
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.08618217457715	34.0	33.0	34.0	31.0	34.0
2	33.223754599275345	34.0	33.0	34.0	31.0	34.0
3	33.29695471248827	34.0	34.0	34.0	31.0	34.0
4	36.56805524042544	37.0	37.0	37.0	35.0	37.0
5	36.54275542498123	37.0	37.0	37.0	35.0	37.0
6	36.58656227997745	37.0	37.0	37.0	35.0	37.0
7	36.58479391269973	37.0	37.0	37.0	35.0	37.0
8	36.55583338986183	37.0	37.0	37.0	35.0	37.0
9	38.352438405381825	39.0	39.0	39.0	37.0	39.0
10	38.392820803110084	39.0	39.0	39.0	37.0	39.0
11	38.45733112209453	39.0	39.0	39.0	37.0	39.0
12	38.41871943075415	39.0	39.0	39.0	37.0	39.0
13	38.45292891678148	39.0	39.0	39.0	37.0	39.0
14	40.03004586995015	41.0	40.0	41.0	38.0	41.0
15	40.01967659462987	41.0	40.0	41.0	38.0	41.0
16	39.98809392930741	41.0	40.0	41.0	38.0	41.0
17	39.988774610362725	41.0	40.0	41.0	38.0	41.0
18	39.99225754538458	41.0	40.0	41.0	38.0	41.0
19	39.99189264420029	41.0	40.0	41.0	38.0	41.0
20	39.96379525300985	41.0	40.0	41.0	38.0	41.0
21	39.93747090731744	41.0	40.0	41.0	38.0	41.0
22	39.916636453160486	41.0	40.0	41.0	38.0	41.0
23	39.876504340218894	41.0	40.0	41.0	38.0	41.0
24	39.854004439631076	41.0	40.0	41.0	38.0	41.0
25	39.81275423203505	41.0	40.0	41.0	38.0	41.0
26	39.73388411580466	41.0	40.0	41.0	38.0	41.0
27	39.65602683427171	41.0	40.0	41.0	37.0	41.0
28	39.61974021842611	41.0	40.0	41.0	37.0	41.0
29	39.581554245134065	41.0	40.0	41.0	37.0	41.0
30	39.535639851887545	41.0	40.0	41.0	37.0	41.0
31	39.48057018149156	41.0	40.0	41.0	37.0	41.0
32	39.43344179007422	41.0	39.0	41.0	37.0	41.0
33	39.36899696617413	41.0	39.0	41.0	36.0	41.0
34	39.32834790988812	41.0	39.0	41.0	36.0	41.0
35	39.251077745004245	41.0	39.0	41.0	36.0	41.0
36	39.20288505846606	41.0	39.0	41.0	35.0	41.0
37	39.15698002166016	41.0	39.0	41.0	35.0	41.0
38	39.07981277762314	41.0	39.0	41.0	35.0	41.0
39	38.997784862682536	41.0	39.0	41.0	35.0	41.0
40	38.92208891893346	40.0	39.0	41.0	35.0	41.0
41	38.86490937117701	40.0	39.0	41.0	35.0	41.0
42	38.79686231763712	40.0	38.0	41.0	35.0	41.0
43	38.093239269916936	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	3.0
19	9.0
20	21.0
21	48.0
22	77.0
23	138.0
24	224.0
25	339.0
26	472.0
27	611.0
28	852.0
29	1256.0
30	1660.0
31	2329.0
32	2982.0
33	4016.0
34	5989.0
35	9647.0
36	15252.0
37	29590.0
38	79100.0
39	272893.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.25248588931799	16.299621298065787	12.266995389613882	28.180897423002342
2	21.22578728600066	18.81018822819423	32.09048613726366	27.873538348541448
3	21.020881236360065	19.53928886373046	28.42205968005651	31.017770219852963
4	15.571456306591847	15.323744541101675	33.639678793393415	35.46512035891306
5	17.2345636273049	32.728829298758164	33.09536786015864	16.941239213778296
6	35.304657402231044	33.369043748377244	14.869021526830764	16.45727732256095
7	31.01192244446368	27.2527385132148	19.582094579580037	22.153244462741483
8	27.59471641798027	30.766315878113648	19.465840804841022	22.173126899065057
9	28.15937760956977	12.867678877601382	17.39034836367549	41.582595149153356
10	19.232163700285138	23.05052711847359	29.788333922009393	27.92897525923188
11	39.528154699389255	19.231695878253994	18.83568452889152	22.404464893465228
12	23.435076828073065	23.303384926306332	26.476621763548714	26.784916482071893
13	34.77999499430426	17.57022593464994	20.460430443050853	27.189348627994935
14	24.227801259844732	19.225380280833566	22.644925417472685	33.90189304184902
15	29.397468614989485	23.865940918755687	19.960796513790225	26.775793952464603
16	26.26914269273683	23.629924704044086	22.341075008245365	27.75985759497372
17	27.15753672987722	23.2544975240519	21.749981871896292	27.837983874174586
18	26.759888003405745	21.79138412165244	23.065965245501307	28.382762629440506
19	28.211539766042204	22.19768755570006	22.835562895163424	26.755209783094315
20	29.48986346614021	21.122398617118076	22.32727425832665	27.060463658415067
21	27.566179274080554	22.188798937108345	21.89056239225474	28.354459396556365
22	28.525916170970238	22.722583874642407	21.223214264829373	27.52828568955798
23	28.307911104457645	22.207745729369634	22.04962188284333	27.434721283329395
24	28.218323185493777	21.71887170682529	21.933602019119885	28.12920308856105
25	28.1902538636252	21.901322298971028	22.704806637458976	27.203617199944794
26	28.031896106083327	22.66761478598312	22.261311351935497	27.03917775599806
27	27.422791821535252	21.91909953615446	23.335196824424052	27.322911817886244
28	26.618605750000583	22.740127200810267	22.1621330813332	28.479133967855947
29	27.673076608196713	23.33309162528391	22.131256827277767	26.86257493924161
30	27.220926615097085	21.996056260277467	23.352974061607483	27.430043063017965
31	27.850381157999877	22.224587322490777	22.5251629775001	27.399868542009248
32	26.002250223969796	22.039797620189326	22.755565327837985	29.20238682800289
33	26.320369205146974	21.75115142697415	24.157627955173293	27.77085141270558
34	26.79988678706846	21.427886403454398	24.065700926053708	27.706525883423428
35	26.189846858458104	22.627616002320398	24.25984706897802	26.922690070243476
36	26.550771555484864	21.38461286557368	24.851408027358232	27.21320755158323
37	26.42983956043442	20.918896033570906	24.818426574162658	27.832837831832013
38	25.533960370795743	20.399145756971134	26.163882735729672	27.90301113650345
39	24.967427891081677	19.760100862429912	27.13671864949136	28.135752596997047
40	24.744042871210933	20.42955418899542	27.95470547094474	26.8716974688489
41	23.289116354356477	19.85904522201664	28.878420071436423	27.97341835219046
42	21.933368108104315	20.98485893996206	29.542025622612645	27.539747329320978
43	21.256897451071662	20.969888634965486	29.31326064938376	28.45995326457909
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	4.0
2	5.0
3	10.0
4	15.0
5	15.0
6	17.5
7	20.0
8	19.5
9	19.0
10	37.5
11	56.0
12	56.0
13	101.5
14	147.0
15	276.0
16	405.0
17	398.5
18	392.0
19	392.0
20	474.5
21	557.0
22	502.5
23	448.0
24	503.0
25	558.0
26	558.0
27	730.5
28	903.0
29	1254.5
30	1606.0
31	2224.0
32	2842.0
33	2842.0
34	3856.0
35	4870.0
36	5924.0
37	6978.0
38	9016.0
39	11054.0
40	11054.0
41	12960.5
42	14867.0
43	17352.0
44	19837.0
45	24788.5
46	29740.0
47	29740.0
48	33271.5
49	36803.0
50	40751.0
51	44699.0
52	45582.0
53	46465.0
54	46465.0
55	43965.5
56	41466.0
57	39419.0
58	37372.0
59	35748.5
60	34125.0
61	34125.0
62	31439.0
63	28753.0
64	24330.0
65	19907.0
66	17332.5
67	14758.0
68	14758.0
69	12626.5
70	10495.0
71	8793.0
72	7091.0
73	5635.0
74	4179.0
75	4179.0
76	3373.5
77	2568.0
78	2051.0
79	1534.0
80	1254.5
81	975.0
82	975.0
83	760.5
84	546.0
85	379.0
86	212.0
87	174.0
88	136.0
89	136.0
90	102.5
91	69.0
92	46.0
93	23.0
94	16.0
95	9.0
96	9.0
97	6.5
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	427513.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.59404503020712
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.09038749500351	51.02978086580424
2	6.680994010777059	7.829329278280303
3	2.0300142791457216	3.5684024425268355
4	1.036089202075727	2.428346294469461
5	0.6207593670311868	1.8186401152374119
6	0.40557970871716814	1.4258733429547221
7	0.31637857867116204	1.2976530479675696
8	0.2154364988120468	1.0098636730034587
9	0.1627731876763836	0.8583785539578339
>10	1.2314814016250113	13.950223397597275
>50	0.128710138969021	5.316274257645799
>100	0.07858936834094282	8.403416017532587
>500	0.002806763155033672	1.063818713022466
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	943	0.2205780876838833	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	697	0.16303597785330504	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	680	0.15905949058859029	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	589	0.13777358817158775	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	588	0.1375396771560163	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	516	0.12069808403487145	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	515	0.1204641730193	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	473	0.11063991036529884	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	472	0.11040599934972738	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	456	0.10666342310058406	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	451	0.1054938680227268	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	434	0.10151738075801205	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	429	0.10034782568015477	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	2.3391101557145632E-4	0.0	0.0	0.0	0.0
9	2.3391101557145632E-4	0.0	0.0	2.3391101557145632E-4	0.0
10	2.3391101557145632E-4	0.0	0.0	4.6782203114291264E-4	0.0
11	2.3391101557145632E-4	0.0	0.0	4.6782203114291264E-4	0.0
12	2.3391101557145632E-4	0.0	0.0	4.6782203114291264E-4	0.0
13	2.3391101557145632E-4	0.0	0.0	4.6782203114291264E-4	0.0
14	2.3391101557145632E-4	0.0	0.0	4.6782203114291264E-4	0.0
15	2.3391101557145632E-4	0.0	0.0	4.6782203114291264E-4	0.0
16	2.3391101557145632E-4	0.0	0.0	9.356440622858253E-4	0.0
17	2.3391101557145632E-4	0.0	0.0	0.0014034660934287378	0.0
18	2.3391101557145632E-4	0.0	0.0	0.0018712881245716506	0.0
19	4.6782203114291264E-4	0.0	0.0	0.0018712881245716506	0.0
20	4.6782203114291264E-4	0.0	0.0	0.002339110155714563	0.0
21	4.6782203114291264E-4	0.0	0.0	0.004210398280286213	2.3391101557145632E-4
22	4.6782203114291264E-4	0.0	0.0	0.006549508436000776	2.3391101557145632E-4
23	4.6782203114291264E-4	0.0	0.0	0.008186885545000971	2.3391101557145632E-4
24	4.6782203114291264E-4	0.0	0.0	0.011227728747429902	2.3391101557145632E-4
25	4.6782203114291264E-4	0.0	0.0	0.012163372809715727	2.3391101557145632E-4
26	4.6782203114291264E-4	0.0	0.0	0.015438127027716117	2.3391101557145632E-4
27	4.6782203114291264E-4	0.0	0.0	0.03508665233571845	2.3391101557145632E-4
28	4.6782203114291264E-4	0.0	0.0	0.14736393981001747	4.6782203114291264E-4
29	4.6782203114291264E-4	0.0	0.0	0.3148442269591802	4.6782203114291264E-4
30	4.6782203114291264E-4	0.0	0.0	0.5489891535462079	4.6782203114291264E-4
31	4.6782203114291264E-4	0.0	0.0	1.2469796240114335	4.6782203114291264E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	475	0.0	29.6	1
AAGAGCG	35	8.860249E-4	26.42857	7
GCTATAC	35	8.860249E-4	26.42857	3
ATAGATT	35	8.860249E-4	26.42857	12
CCGTTAC	35	8.860249E-4	26.42857	14
CGTTAGA	45	1.3213136E-4	24.666666	1
TAGAACA	50	2.6982572E-4	22.2	4
CTAAATC	50	2.6982572E-4	22.2	3
GCGGTAA	415	0.0	21.843374	23
ATACGGC	85	5.1615643E-8	21.764706	29
ATACACA	115	1.3460522E-10	20.913042	37
GAATATA	45	0.003821937	20.555555	1
TAGATTG	45	0.003821937	20.555555	13
GATTGGT	45	0.003821937	20.555555	15
GTCCAAT	45	0.003821937	20.555555	20
TAATACG	425	0.0	20.458824	27
AGATTGG	55	5.135833E-4	20.181818	14
CGGTAAT	450	0.0	20.144444	24
TAAACTA	65	6.8891764E-5	19.923077	5
CTTATAC	545	0.0	19.688074	37
>>END_MODULE