FastQCFastQC Report
Fri 10 Feb 2017
ERR1632409.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632409.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98726
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6440.6523104349411503No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4470.4527682677308915No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3840.38895529039969207No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3550.3595810627392987No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3090.3129874602435022No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2980.3018455118205944No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2900.2937422766039341No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2850.28867775459352146No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2810.28462613698519135No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2790.28260032818102626No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2560.259303526933128No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2540.257277718128963No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2460.24917448291230274No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2450.2481615785102202No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2400.24309705649980753No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC2390.242084152097725No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2340.23701963008731236No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2330.23600672568522982No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2230.2258776816644045No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2210.22385187286023947No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2190.22182606405607438No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC2140.21676154204566173No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2110.21372282883941413No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG1920.19447764519984603No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1850.18738731438526832No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1850.18738731438526832No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1850.18738731438526832No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1740.17624536596236046No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT1700.17219374835403034RNA PCR Primer, Index 41 (95% over 22bp)
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1630.16510341753945262No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT1630.16510341753945262No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1630.16510341753945262No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1600.16206470433320505No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC1580.16003889552903997No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1580.16003889552903997No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1570.15902599112695745No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1540.15598727792070985No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG1520.15396146911654476No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1500.1519356603123797No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1480.14990985150821465No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT1480.14990985150821465No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1460.1478840427040496No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1450.14687113830196705No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1450.14687113830196705No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT1410.14281952069363693No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1410.14281952069363693No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC1380.13978080748738936No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG1370.13876790308530682No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1310.1326904766728116No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1300.1316775722707291No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA1300.1316775722707291No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG1290.13066466786864656No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1290.13066466786864656No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG1250.1266130502603164No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1220.12357433705406883No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1210.12256143265198631No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC1200.12154852824990377No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT1190.12053562384782125No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA1190.12053562384782125No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1180.11952271944573871No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA1170.11850981504365618No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC1170.11850981504365618No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC1160.11749691064157365No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC1140.11547110183740858No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG1130.11445819743532606No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA1120.11344529303324352No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC1110.11243238863116098No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT1100.11141948422907846No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1100.11141948422907846No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT1090.11040657982699592No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT1090.11040657982699592No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA1090.11040657982699592No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC1080.1093936754249134No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC1080.1093936754249134No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA1060.10736786662074833No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT1050.1063549622186658No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA1050.1063549622186658No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC1000.10129044020825313No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG1000.10129044020825313No Hit
CCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTC990.10027753580617062No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGATC200.001833797137.03
GCCGTCT352.366419E-531.71428536
TATACAC551.819717E-830.27272837
GTGAGTC250.005472333629.623
GAGTCGA250.005472333629.625
ATACGGC250.005472333629.629
GGAGGGC250.005472333629.633
CCCCTAA250.005472333629.632
GACCCCT250.005472333629.630
TGAGTCG250.005472333629.624
GGTATCA2100.028.1904751
TGCCGTC405.8861246E-527.7535
TATGCCG405.8861246E-527.7533
CGAGCCC405.8861246E-527.755
TCTCCGA405.8861246E-527.751
CTCCGAG405.8861246E-527.752
CCCACGA508.981655E-625.9000029
GTATGCC451.3112786E-424.66666832
ATCTCGT451.3112786E-424.66666827
CTAAGAC451.3112786E-424.66666820
Produced by FastQC (version 0.11.5)