FastQCFastQC Report
Fri 10 Feb 2017
ERR1632408.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632408.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2291246
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA46380.20242261197619113No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA38640.1686418656050027No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA31020.13538485173569315No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA28770.12556486732546396No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA27750.1211131410594934No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA25840.11277706540458773No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA25170.10985289226909725No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG25010.10915458226659205No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC24760.1080634728876777No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT23380.10204054911607047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25400.029.0610241
ATACGGC2950.020.06779729
GTATCAA36700.019.9114462
CTTATAC29500.018.81355937
GCGGTAA18000.018.70555523
TGTTACG1258.591087E-917.7616
GGTAATA20000.017.3925
CGCGGTA19550.017.31713522
GTATTAT752.0683964E-417.2666661
ATCTTAC1851.8189894E-1217.01
CGGTAAT20150.016.70967924
TCTTATA53050.016.70405437
TAATACG19500.016.50769227
CCGCGGT21600.016.187521
GTAACGT13900.016.10431726
TAGGTTG1151.2434375E-616.0869565
GACCGTT2650.016.0566027
TATACAC9450.016.0529137
GGACCGT4400.015.9772736
CTATATG700.002593547615.8571431
Produced by FastQC (version 0.11.5)