FastQCFastQC Report
Fri 10 Feb 2017
ERR1632407.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632407.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468445
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8810.18806903692002264No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7080.15113834068033602No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5680.12125222811642776No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5530.11805014462743758No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5300.11314028327765266No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4710.10054542155429132No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATT303.596251E-430.83333418
ATACGGC801.8189894E-1230.062529
GGTATCA5100.028.2941171
TAAACTA358.861518E-426.4285725
TACGGCT1103.6379788E-1223.54545430
GGTAACG2100.022.0238125
GCGGTAA3400.020.67647223
TGGGTAA450.003822475220.55555510
GTCTTAA450.003822475220.5555551
GTAACGT2250.020.55555326
AACGTCA2350.020.46808628
CGCGGTA3650.020.27397222
TATTAGA555.136842E-420.1818182
GTATCAA7150.020.1818182
CGTCAAT2200.020.18181830
GCGGGTA2350.019.68085122
CGGTAAT3650.019.26027324
TATACAC1850.019.00000237
CGGGTAA2550.018.86274523
CTTATAC5500.018.83636537
Produced by FastQC (version 0.11.5)