##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632407.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	468445
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.063648880871824	34.0	33.0	34.0	31.0	34.0
2	33.201782493142204	34.0	33.0	34.0	31.0	34.0
3	33.281435387292	34.0	34.0	34.0	31.0	34.0
4	36.56328491071524	37.0	37.0	37.0	35.0	37.0
5	36.527620104814865	37.0	37.0	37.0	35.0	37.0
6	36.564783485788084	37.0	37.0	37.0	35.0	37.0
7	36.56022371889976	37.0	37.0	37.0	35.0	37.0
8	36.53363788705184	37.0	37.0	37.0	35.0	37.0
9	38.32614714640993	39.0	39.0	39.0	37.0	39.0
10	38.359179839682355	39.0	39.0	39.0	37.0	39.0
11	38.427294559660155	39.0	39.0	39.0	37.0	39.0
12	38.38429698256999	39.0	39.0	39.0	37.0	39.0
13	38.416802399427894	39.0	39.0	39.0	37.0	39.0
14	39.987864103576726	41.0	40.0	41.0	38.0	41.0
15	39.97213973892346	41.0	40.0	41.0	38.0	41.0
16	39.95039118786624	41.0	40.0	41.0	38.0	41.0
17	39.94449295007952	41.0	40.0	41.0	38.0	41.0
18	39.94307976389971	41.0	40.0	41.0	38.0	41.0
19	39.95155247681158	41.0	40.0	41.0	38.0	41.0
20	39.914687956963995	41.0	40.0	41.0	38.0	41.0
21	39.88822593901099	41.0	40.0	41.0	38.0	41.0
22	39.855797372156815	41.0	40.0	41.0	38.0	41.0
23	39.81996819263734	41.0	40.0	41.0	38.0	41.0
24	39.80341342099926	41.0	40.0	41.0	38.0	41.0
25	39.76401925519538	41.0	40.0	41.0	38.0	41.0
26	39.69294581007375	41.0	40.0	41.0	38.0	41.0
27	39.60328320293738	41.0	40.0	41.0	37.0	41.0
28	39.55691916873913	41.0	40.0	41.0	37.0	41.0
29	39.52043249474325	41.0	40.0	41.0	37.0	41.0
30	39.48012039833919	41.0	40.0	41.0	37.0	41.0
31	39.42506164010716	41.0	39.0	41.0	37.0	41.0
32	39.36797276094312	41.0	39.0	41.0	36.0	41.0
33	39.29780657281004	41.0	39.0	41.0	36.0	41.0
34	39.26219940441247	41.0	39.0	41.0	36.0	41.0
35	39.1912455037411	41.0	39.0	41.0	35.0	41.0
36	39.1429474111155	41.0	39.0	41.0	35.0	41.0
37	39.08485948190289	41.0	39.0	41.0	35.0	41.0
38	39.01195657974789	41.0	39.0	41.0	35.0	41.0
39	38.93940377205435	40.0	39.0	41.0	35.0	41.0
40	38.86611661988067	40.0	39.0	41.0	35.0	41.0
41	38.818777017579436	40.0	38.0	41.0	35.0	41.0
42	38.74822657942768	40.0	38.0	41.0	35.0	41.0
43	38.01319898814162	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	2.0
16	0.0
17	3.0
18	3.0
19	13.0
20	22.0
21	37.0
22	82.0
23	162.0
24	258.0
25	401.0
26	534.0
27	759.0
28	1067.0
29	1510.0
30	1897.0
31	2644.0
32	3435.0
33	4650.0
34	6871.0
35	10844.0
36	17593.0
37	33569.0
38	90683.0
39	291405.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.63287045437565	16.008282722624855	11.866067521267171	28.49277930173233
2	21.083371580441675	18.659394379276115	31.850697520520015	28.40653651976219
3	21.34103256518908	19.113236345782322	28.189435259208658	31.356295829819935
4	16.005294111368464	14.760964467546883	32.78015562125757	36.45358579982709
5	17.698342388113865	32.537010748326914	32.59635602898953	17.168290834569692
6	36.66705803242643	32.51203449711279	14.559019735507903	16.261887734952875
7	31.65152792750483	27.096457428300013	19.183682182540107	22.06833246165505
8	28.39244735241063	30.5852341256711	19.134583569042256	21.887734952876002
9	28.126034006126655	12.59571561229173	17.176189307175868	42.102061074405746
10	19.154223014441396	23.326751272828187	29.91834687103075	27.600678841699665
11	40.092646948948115	18.865822028199684	18.61008229354567	22.431448729306535
12	23.806636851711513	22.992453756577614	26.376842532207622	26.824066859503247
13	35.02033323015509	17.271397922915178	20.522793497635796	27.18547534929394
14	24.681873005368825	18.567814791490996	22.36762053175933	34.38269167138085
15	29.313366563844205	23.682396012338693	19.712239430456084	27.29199799336101
16	26.9064671412866	23.199094877733778	21.676183970370055	28.218254010609574
17	27.253146047027933	23.037496397656074	21.711620360981545	27.997737194334448
18	27.213013267299257	21.460150070979516	22.78794735774744	28.538889303973786
19	28.608267779568575	22.034176904439153	22.495276926853737	26.862278389138535
20	29.488413794575667	21.229813532004822	22.135576214923844	27.146196458495663
21	27.99731024986925	21.7916724482063	21.882184674828423	28.32883262709603
22	28.73955320261717	22.343284697243007	21.060956996018742	27.85620510412108
23	28.610189029661964	21.7916724482063	21.976112457172135	27.6220260649596
24	28.383268046408865	21.588660355004322	21.602962994588477	28.425108603998332
25	28.62812069720031	21.699025499258184	22.194281078888665	27.47857272465284
26	28.508576246944678	21.829883977841583	22.02328982057659	27.63824995463715
27	27.448473139856333	21.662094803018498	23.17668029331085	27.71275176381432
28	27.039887286661184	22.284579833278187	22.04186190481273	28.633670975247895
29	27.668776483898856	22.89297569618632	21.998527041595064	27.43972077831976
30	27.420935221851018	21.909722592833734	23.0618322321724	27.607509953142845
31	28.094440115701953	21.97077565135715	22.313825529144296	27.620958703796607
32	26.485286426368088	21.930429399395873	22.474783592524204	29.109500581711835
33	26.891524085004644	21.522697435131125	23.640768926981824	27.94500955288241
34	27.330209522996295	21.204196864092903	23.465081279552564	28.00051233335824
35	26.49446573236986	22.257041915272872	23.934079774573323	27.314412577783948
36	26.71690379873838	21.0428118562478	24.667570365784673	27.572713979229153
37	26.83751561015701	20.78472392703519	24.51109521928935	27.86666524351845
38	25.804096532143582	20.493334329537085	25.738987501200782	27.96358163711855
39	25.255899838828466	19.766888322001517	26.720532826692565	28.256679012477452
40	25.04242760622912	20.357565989603902	27.48881939181761	27.11118701234937
41	23.754336154724673	19.772652072281698	28.28955373629775	28.183458036695875
42	22.37359775427211	20.60583419611694	29.152195028231702	27.868373021379245
43	21.808323282349047	20.546061970989125	29.09156891417349	28.554045832488338
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	4.5
2	7.0
3	8.5
4	10.0
5	10.0
6	16.0
7	22.0
8	21.0
9	20.0
10	35.0
11	50.0
12	50.0
13	89.0
14	128.0
15	213.5
16	299.0
17	308.0
18	317.0
19	317.0
20	406.0
21	495.0
22	465.5
23	436.0
24	541.0
25	646.0
26	646.0
27	824.0
28	1002.0
29	1402.0
30	1802.0
31	2346.5
32	2891.0
33	2891.0
34	3948.5
35	5006.0
36	6070.0
37	7134.0
38	9071.5
39	11009.0
40	11009.0
41	13127.5
42	15246.0
43	17887.5
44	20529.0
45	25606.5
46	30684.0
47	30684.0
48	34266.5
49	37849.0
50	42081.0
51	46313.0
52	47879.5
53	49446.0
54	49446.0
55	47383.0
56	45320.0
57	43807.0
58	42294.0
59	40856.0
60	39418.0
61	39418.0
62	36690.5
63	33963.0
64	29146.5
65	24330.0
66	21305.0
67	18280.0
68	18280.0
69	15450.5
70	12621.0
71	10513.0
72	8405.0
73	6735.5
74	5066.0
75	5066.0
76	4048.5
77	3031.0
78	2450.5
79	1870.0
80	1508.5
81	1147.0
82	1147.0
83	922.5
84	698.0
85	522.0
86	346.0
87	261.5
88	177.0
89	177.0
90	133.5
91	90.0
92	62.0
93	34.0
94	22.0
95	10.0
96	10.0
97	6.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	468445.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.67203309315054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.38789280640148	53.27716984003933
2	7.16748887759646	8.840672225078345
3	2.249313695649778	4.161592460249695
4	1.0938860191109558	2.6984869908298226
5	0.6728684471512615	2.0748582570024707
6	0.4437781858451777	1.6421221778077262
7	0.30098474233458644	1.2993638692854401
8	0.20460072580512856	1.0094514186189205
9	0.19656035915908984	1.091004927737485
>10	1.1304966126657636	13.05605701169273
>50	0.0989883917404337	4.2935059388340076
>100	0.05140449809082411	5.8616937679621275
>500	0.0017366384490143283	0.6940211148619512
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	881	0.18806903692002264	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	708	0.15113834068033602	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	568	0.12125222811642776	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	553	0.11805014462743758	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	530	0.11314028327765266	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	471	0.10054542155429132	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	4.2694446519868926E-4	0.0
19	0.0	0.0	0.0	0.0010673611629967233	0.0
20	0.0	0.0	0.0	0.001707777860794757	0.0
21	0.0	0.0	0.0	0.0029886112563908248	0.0
22	0.0	0.0	0.0	0.004055972419387548	0.0
23	0.0	0.0	0.0	0.0049098613497849265	0.0
24	0.0	0.0	0.0	0.007471528140977062	0.0
25	0.0	0.0	0.0	0.00875236153657313	0.0
26	0.0	0.0	0.0	0.011314028327765267	0.0
27	0.0	0.0	0.0	0.03180736265730235	0.0
28	0.0	0.0	0.0	0.12786986732700745	0.0
29	0.0	0.0	0.0	0.2770869579139493	0.0
30	0.0	0.0	0.0	0.46558293929917066	0.0
31	0.0	0.0	0.0	1.0763269967658957	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCATT	30	3.596251E-4	30.833334	18
ATACGGC	80	1.8189894E-12	30.0625	29
GGTATCA	510	0.0	28.294117	1
TAAACTA	35	8.861518E-4	26.428572	5
TACGGCT	110	3.6379788E-12	23.545454	30
GGTAACG	210	0.0	22.02381	25
GCGGTAA	340	0.0	20.676472	23
TGGGTAA	45	0.0038224752	20.555555	10
GTCTTAA	45	0.0038224752	20.555555	1
GTAACGT	225	0.0	20.555553	26
AACGTCA	235	0.0	20.468086	28
CGCGGTA	365	0.0	20.273972	22
TATTAGA	55	5.136842E-4	20.181818	2
GTATCAA	715	0.0	20.181818	2
CGTCAAT	220	0.0	20.181818	30
GCGGGTA	235	0.0	19.680851	22
CGGTAAT	365	0.0	19.260273	24
TATACAC	185	0.0	19.000002	37
CGGGTAA	255	0.0	18.862745	23
CTTATAC	550	0.0	18.836365	37
>>END_MODULE