FastQCFastQC Report
Fri 10 Feb 2017
ERR1632406.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632406.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14014
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT680.4852290566576281TruSeq Adapter, Index 10 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA590.4210075638647067No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA450.3211074639646068No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA360.25688597117168543No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA350.24975024975024976No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC330.23547880690737832No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA320.22834308548594262No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG320.22834308548594262No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT280.1998001998001998No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA270.19266447837876408No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC260.1855287569573284No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA260.1855287569573284No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA260.1855287569573284No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC250.17839303553589267No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC250.17839303553589267No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT240.17125731411445697No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC230.16412159269302126No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA230.16412159269302126No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT230.16412159269302126No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT220.15698587127158556No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT220.15698587127158556No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT220.15698587127158556No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC210.14985014985014986No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC200.14271442842871412No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA200.14271442842871412No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG190.13557870700727845No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC190.13557870700727845No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC190.13557870700727845RNA PCR Primer, Index 10 (95% over 22bp)
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC180.12844298558584272No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC180.12844298558584272No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC180.12844298558584272No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC180.12844298558584272No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA170.12130726416440703No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT170.12130726416440703No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG170.12130726416440703No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA160.11417154274297131No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT160.11417154274297131No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC160.11417154274297131No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC160.11417154274297131No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG160.11417154274297131No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA160.11417154274297131No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT150.10703582132153562No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA150.10703582132153562No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA150.10703582132153562No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA150.10703582132153562No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA150.10703582132153562No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA150.10703582132153562No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG150.10703582132153562No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA303.4226518E-430.8333341
TCTCTTA652.383067E-622.7692337
TATCAAC450.00364302620.5555552
TCAACGC500.00670191818.54
ATCAACG500.00670191818.53
GTCTCTT1009.761851E-514.837
TGTCTCT1102.1962737E-413.45454536
CTGTCTC1350.001233778710.96296235
Produced by FastQC (version 0.11.5)