##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632406.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14014
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.884187241330096	34.0	31.0	34.0	31.0	34.0
2	33.034822320536605	34.0	33.0	34.0	31.0	34.0
3	33.113386613386616	34.0	33.0	34.0	31.0	34.0
4	36.46310832025118	37.0	37.0	37.0	35.0	37.0
5	36.405237619523334	37.0	37.0	37.0	35.0	37.0
6	36.42735835592978	37.0	37.0	37.0	35.0	37.0
7	36.45711431425717	37.0	37.0	37.0	35.0	37.0
8	36.41622663051235	37.0	37.0	37.0	35.0	37.0
9	38.18652775795633	39.0	39.0	39.0	37.0	39.0
10	38.21414299985729	39.0	39.0	39.0	37.0	39.0
11	38.26166690452405	39.0	39.0	39.0	37.0	39.0
12	38.21078921078921	39.0	39.0	39.0	37.0	39.0
13	38.23833309547595	39.0	39.0	39.0	37.0	39.0
14	39.755744255744254	41.0	40.0	41.0	38.0	41.0
15	39.75874125874126	41.0	40.0	41.0	38.0	41.0
16	39.690095618667044	41.0	40.0	41.0	38.0	41.0
17	39.700156985871274	41.0	40.0	41.0	38.0	41.0
18	39.69801626944484	41.0	40.0	41.0	38.0	41.0
19	39.69715998287427	41.0	40.0	41.0	38.0	41.0
20	39.681532752961324	41.0	40.0	41.0	38.0	41.0
21	39.63251034679606	41.0	40.0	41.0	37.0	41.0
22	39.59526188097617	41.0	40.0	41.0	37.0	41.0
23	39.57521050378193	41.0	40.0	41.0	37.0	41.0
24	39.56222349079492	41.0	40.0	41.0	37.0	41.0
25	39.538889681746824	41.0	39.0	41.0	37.0	41.0
26	39.417582417582416	41.0	39.0	41.0	37.0	41.0
27	39.30498073355216	40.0	39.0	41.0	36.0	41.0
28	39.29713143998858	40.0	39.0	41.0	36.0	41.0
29	39.256243756243755	40.0	39.0	41.0	36.0	41.0
30	39.23676323676324	40.0	39.0	41.0	36.0	41.0
31	39.178607107178536	40.0	39.0	41.0	36.0	41.0
32	39.15063507920651	40.0	39.0	41.0	35.0	41.0
33	39.00171257314114	40.0	39.0	41.0	35.0	41.0
34	38.945839874411305	40.0	39.0	41.0	35.0	41.0
35	38.84037391180248	40.0	39.0	41.0	35.0	41.0
36	38.82524618238904	40.0	39.0	41.0	35.0	41.0
37	38.78121878121878	40.0	38.0	41.0	35.0	41.0
38	38.71699728842586	40.0	38.0	41.0	35.0	41.0
39	38.55829884401313	40.0	38.0	41.0	35.0	41.0
40	38.4552590266876	40.0	38.0	41.0	35.0	41.0
41	38.404024546881686	40.0	38.0	41.0	35.0	41.0
42	38.332025117739406	40.0	38.0	41.0	35.0	41.0
43	37.55387469673184	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	0.0
20	2.0
21	2.0
22	0.0
23	5.0
24	12.0
25	10.0
26	26.0
27	28.0
28	50.0
29	55.0
30	71.0
31	100.0
32	129.0
33	145.0
34	261.0
35	416.0
36	639.0
37	1277.0
38	3381.0
39	7403.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.86527757956329	16.29085200513772	12.644498358784073	29.199372056514918
2	21.307264164407023	19.65177679463394	31.04752390466676	27.993435136292277
3	21.150278293135436	19.444840873412303	28.892536035393178	30.512344798059083
4	15.89125160553732	15.470244041672613	34.030255458826886	34.60824889396318
5	16.59055230483802	32.5531611245897	32.64592550306836	18.210361067503925
6	33.38804053089767	34.13015555872699	15.748537177108608	16.73326673326673
7	29.84158698444413	27.472527472527474	19.230769230769234	23.455116312259168
8	27.01584130155559	30.08420151277294	20.436706150991863	22.463251034679608
9	28.129013843299553	12.41615527329813	17.40402454688169	42.05080633652062
10	19.587555301841018	22.77722277722278	28.607107178535752	29.028114742400458
11	39.57471100328243	19.16654773797631	18.431568431568433	22.827172827172827
12	22.72013700585129	23.761952333380904	26.54488368774083	26.973026973026975
13	36.71328671328671	17.11859569002426	18.78121878121878	27.386898815470246
14	24.397031539888683	19.38061938061938	22.80576566290852	33.416583416583414
15	30.462394748109034	23.626373626373624	19.252176395033537	26.6590552304838
16	26.009704581133153	23.426573426573427	22.20636506350792	28.3573569287855
17	26.245183388040534	23.29099471956615	21.763950335378908	28.69987155701441
18	27.20850577993435	20.358213215356074	22.86998715570144	29.563293849008133
19	27.9577565291851	22.27772227772228	22.184957899243614	27.579563293849006
20	30.869130869130867	19.872984158698443	21.51420008562866	27.74368488654203
21	27.536748965320395	21.642643071214497	21.678321678321677	29.142286285143427
22	29.598972456115312	22.670186955901244	20.686456400742117	27.044384187241334
23	28.728414442700156	21.071785357499643	21.428571428571427	28.771228771228774
24	28.02911374339946	20.772085057799345	22.320536606250894	28.878264592550305
25	29.149422006564862	21.328671328671327	22.62023690595119	26.90166975881262
26	28.87112887112887	22.59169402026545	21.314399885828458	27.22277722277722
27	27.22277722277722	21.3429427715142	24.839446268017696	26.59483373769088
28	26.159554730983302	22.83430854859426	21.65691451405737	29.34922220636506
29	27.286998715570142	23.326673326673326	21.457114314257172	27.929213643499356
30	27.522477522477523	21.22163550734979	23.761952333380904	27.493934636791778
31	29.10660767803625	21.77822177822178	21.999429142286285	27.11574140145569
32	26.64478378764093	21.485657199942914	22.249179392036535	29.620379620379623
33	25.34608248893963	21.9423433709148	24.304267161410017	28.40730697873555
34	27.11574140145569	22.07792207792208	22.898530041387186	27.907806479235052
35	25.51733980305409	22.962751534180107	24.496931639788784	27.022977022977024
36	26.66619095190524	21.69259312116455	24.375624375624376	27.265591551305835
37	27.022977022977024	21.371485657199944	23.897531040388184	27.708006279434848
38	26.2594548308834	20.90766376480662	25.445982588839733	27.386898815470246
39	26.61624090195519	19.623233908948194	25.71000428143285	28.050520907663767
40	24.810903382331954	21.16454973597831	27.78649921507064	26.238047666619096
41	24.38989581846725	19.99429142286285	27.629513343799054	27.986299414870842
42	22.07792207792208	20.829170829170828	29.613243898958185	27.479663193948905
43	21.136006850292567	21.057513914656774	28.13614956472099	29.67032967032967
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	2.0
4	3.0
5	3.0
6	2.0
7	1.0
8	1.0
9	1.0
10	1.0
11	1.0
12	1.0
13	3.5
14	6.0
15	12.5
16	19.0
17	19.5
18	20.0
19	20.0
20	22.0
21	24.0
22	20.0
23	16.0
24	17.5
25	19.0
26	19.0
27	25.0
28	31.0
29	35.0
30	39.0
31	63.5
32	88.0
33	88.0
34	101.0
35	114.0
36	124.5
37	135.0
38	168.5
39	202.0
40	202.0
41	284.0
42	366.0
43	445.5
44	525.0
45	738.5
46	952.0
47	952.0
48	1093.5
49	1235.0
50	1427.0
51	1619.0
52	1642.0
53	1665.0
54	1665.0
55	1575.0
56	1485.0
57	1374.5
58	1264.0
59	1260.0
60	1256.0
61	1256.0
62	1134.0
63	1012.0
64	819.0
65	626.0
66	542.0
67	458.0
68	458.0
69	386.5
70	315.0
71	266.5
72	218.0
73	170.0
74	122.0
75	122.0
76	100.0
77	78.0
78	57.5
79	37.0
80	31.5
81	26.0
82	26.0
83	20.5
84	15.0
85	12.0
86	9.0
87	7.0
88	5.0
89	5.0
90	4.0
91	3.0
92	2.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	14014.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.04124446981591
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.49159828057836	64.63536463536464
2	5.930050801094177	8.662765805622948
3	1.8561938257131692	4.067361210218353
4	1.0844079718640094	3.1682603111174537
5	0.6252442360296991	2.283430854859426
6	0.4787026182102384	2.097902097902098
7	0.3907776475185619	1.998001998001998
8	0.16608050019538884	0.9704581133152562
9	0.16608050019538884	1.0917653774796632
>10	0.7913247362250878	10.118452975595833
>50	0.019538882375928098	0.9062366205223348
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT	68	0.4852290566576281	TruSeq Adapter, Index 10 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	59	0.4210075638647067	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	45	0.3211074639646068	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	36	0.25688597117168543	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	35	0.24975024975024976	No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC	33	0.23547880690737832	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	32	0.22834308548594262	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	32	0.22834308548594262	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	28	0.1998001998001998	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	27	0.19266447837876408	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	26	0.1855287569573284	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	26	0.1855287569573284	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	26	0.1855287569573284	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	25	0.17839303553589267	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	25	0.17839303553589267	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	24	0.17125731411445697	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	23	0.16412159269302126	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	23	0.16412159269302126	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	23	0.16412159269302126	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	22	0.15698587127158556	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	22	0.15698587127158556	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	22	0.15698587127158556	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	21	0.14985014985014986	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	20	0.14271442842871412	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	20	0.14271442842871412	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	19	0.13557870700727845	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	19	0.13557870700727845	No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC	19	0.13557870700727845	RNA PCR Primer, Index 10 (95% over 22bp)
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	18	0.12844298558584272	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	18	0.12844298558584272	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	18	0.12844298558584272	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	18	0.12844298558584272	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	17	0.12130726416440703	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	17	0.12130726416440703	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	17	0.12130726416440703	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	16	0.11417154274297131	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	16	0.11417154274297131	No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC	16	0.11417154274297131	No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC	16	0.11417154274297131	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	16	0.11417154274297131	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	16	0.11417154274297131	No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT	15	0.10703582132153562	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	15	0.10703582132153562	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	15	0.10703582132153562	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	15	0.10703582132153562	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	15	0.10703582132153562	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	15	0.10703582132153562	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	15	0.10703582132153562	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.007135721421435707	0.0
24	0.0	0.0	0.0	0.014271442842871414	0.0
25	0.0	0.0	0.0	0.014271442842871414	0.0
26	0.0	0.0	0.0	0.014271442842871414	0.0
27	0.0	0.0	0.0	0.03567860710717854	0.0
28	0.0	0.0	0.0	0.11417154274297131	0.0
29	0.0	0.0	0.0	0.24261452832881406	0.0
30	0.0	0.0	0.0	0.4995004995004995	0.0
31	0.0	0.0	0.0	0.9775938347366919	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	30	3.4226518E-4	30.833334	1
TCTCTTA	65	2.383067E-6	22.76923	37
TATCAAC	45	0.003643026	20.555555	2
TCAACGC	50	0.006701918	18.5	4
ATCAACG	50	0.006701918	18.5	3
GTCTCTT	100	9.761851E-5	14.8	37
TGTCTCT	110	2.1962737E-4	13.454545	36
CTGTCTC	135	0.0012337787	10.962962	35
>>END_MODULE