FastQCFastQC Report
Fri 10 Feb 2017
ERR1632405.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632405.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68513
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2600.3794900237911053No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2390.3488389064849007No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2060.3006728650037219No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1740.2539664005371243No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1740.2539664005371243No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1720.251047246507962No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1640.23937063039131257No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1540.22477486024550086No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1540.22477486024550086No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1410.2058003590559456No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT1400.20434078204136444TruSeq Adapter, Index 7 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1350.19704289696845853No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1320.19266416592471503No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1310.19120458891013384No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1300.18974501189555265No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1200.17514924174974092No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1130.1649322026476727No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1120.16347262563309153No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1060.1547151635456045No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1000.14595770145811746No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC990.14449812444353627No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA960.14011939339979274No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT960.14011939339979274No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA950.13865981638521158No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT950.13865981638521158No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT950.13865981638521158No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA900.1313619313123057No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG890.12990235429772454No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA870.12698320026856216No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT860.125523623253981No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC820.11968531519565628No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC810.11822573818107514No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA790.11530658415191278No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG790.11530658415191278No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA780.1138470071373316No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC780.1138470071373316No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG780.1138470071373316No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC760.11092785310816924No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC750.10946827609358807No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG740.1080086990790069No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC730.10654912206442572No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC720.10508954504984455No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC720.10508954504984455No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT720.10508954504984455No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA710.10362996803526339No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG710.10362996803526339No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC700.10217039102068222No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA690.10071081400610103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT308.198353E-637.037
ATACGGC401.571183E-632.37529
GCCGTCT352.3559172E-531.71428536
GGTATCA1100.030.2727281
GAGTTCC250.00546077929.615
GTATGCC405.860212E-527.7532
TGCCGTC405.860212E-527.7535
TATGCCG405.860212E-527.7533
ATGCCGT405.860212E-527.7534
CGTATGC405.860212E-527.7531
CCTATAC358.784178E-426.428573
CCAGTCA358.784178E-426.4285714
AGTCAAA358.784178E-426.4285716
TACGGCT508.929253E-625.90000230
CGGCTGT508.929253E-625.90000232
TAGCGCT451.3055529E-424.66666819
ATCTCGT451.3055529E-424.66666827
ATGGTCT451.3055529E-424.66666836
GAGCCCA451.3055529E-424.6666686
CACGAGA451.3055529E-424.66666811
Produced by FastQC (version 0.11.5)