FastQCFastQC Report
Fri 10 Feb 2017
ERR1632404.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632404.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99563
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2200.22096561975834395No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2160.21694806303546496No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2020.20288661450538853No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1870.18782077679459236No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1790.17978566334883442No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1630.16371543645731848No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1590.1596978797344395No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1480.1486495987465223No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1480.1486495987465223No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1460.1466408203850828No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1410.1416188744814841No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1360.13659692857788536No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1310.13157498267428663No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1230.12353986922852868No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1190.11952231250564968No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1180.11851792332492994No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT1070.10746964233701274RNA PCR Primer, Index 39 (95% over 23bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1020.10244769643341402No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGGC303.5761172E-430.8333344
CTCTATG303.5761172E-430.8333341
TCTATGG303.5761172E-430.8333342
TGAGTAT250.0054725629.5999983
AATCTGA250.0054725629.59999824
TGTACTG250.0054725629.59999810
GCATGTA250.0054725629.5999987
GGTATCA1050.026.4285741
CTATGGG358.812376E-426.428573
ATACGGC358.812376E-426.4285729
GAACTCG451.3113898E-424.66666827
CCGTGCC901.382432E-1024.6666689
CAATGAG451.3113898E-424.66666833
GCGGTAA851.9226718E-923.94117723
CGTGCCA851.9226718E-923.94117710
CCGCGGT851.9226718E-923.94117721
TGAGCAA400.001918890223.12536
CGCGGTA903.7234713E-922.61111322
CGGTAAT903.7234713E-922.61111324
AATGAGC502.6781566E-422.19999934