##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632404.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	99563
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.06658095878991	34.0	33.0	34.0	31.0	34.0
2	33.211966292699096	34.0	33.0	34.0	31.0	34.0
3	33.27593583961914	34.0	34.0	34.0	31.0	34.0
4	36.554432871649105	37.0	37.0	37.0	35.0	37.0
5	36.52812791900605	37.0	37.0	37.0	35.0	37.0
6	36.57189919950182	37.0	37.0	37.0	35.0	37.0
7	36.57393810953869	37.0	37.0	37.0	35.0	37.0
8	36.544549682110826	37.0	37.0	37.0	35.0	37.0
9	38.348523045709754	39.0	39.0	39.0	37.0	39.0
10	38.38552474312747	39.0	39.0	39.0	37.0	39.0
11	38.454907947731584	39.0	39.0	39.0	37.0	39.0
12	38.41267338268232	39.0	39.0	39.0	37.0	39.0
13	38.43339393148057	39.0	39.0	39.0	37.0	39.0
14	40.000924038046264	41.0	40.0	41.0	38.0	41.0
15	39.99150286753111	41.0	40.0	41.0	38.0	41.0
16	39.96863292588612	41.0	40.0	41.0	38.0	41.0
17	39.95673091409459	41.0	40.0	41.0	38.0	41.0
18	39.956369333989535	41.0	40.0	41.0	38.0	41.0
19	39.95260287456183	41.0	40.0	41.0	38.0	41.0
20	39.918162369554956	41.0	40.0	41.0	38.0	41.0
21	39.902905697899826	41.0	40.0	41.0	38.0	41.0
22	39.869108001968605	41.0	40.0	41.0	38.0	41.0
23	39.82983638500246	41.0	40.0	41.0	38.0	41.0
24	39.813374446330464	41.0	40.0	41.0	38.0	41.0
25	39.76828741600796	41.0	40.0	41.0	38.0	41.0
26	39.68109639122967	41.0	40.0	41.0	37.0	41.0
27	39.59856573224993	41.0	40.0	41.0	37.0	41.0
28	39.56226710725872	41.0	40.0	41.0	37.0	41.0
29	39.532717977561944	41.0	40.0	41.0	37.0	41.0
30	39.47313761136165	41.0	40.0	41.0	37.0	41.0
31	39.41716300232014	41.0	39.0	41.0	36.0	41.0
32	39.36285567931862	41.0	39.0	41.0	36.0	41.0
33	39.292056285969686	41.0	39.0	41.0	36.0	41.0
34	39.257043279129796	41.0	39.0	41.0	35.0	41.0
35	39.16874742625272	41.0	39.0	41.0	35.0	41.0
36	39.1363357873909	41.0	39.0	41.0	35.0	41.0
37	39.0759217781706	41.0	39.0	41.0	35.0	41.0
38	38.99488765907014	41.0	39.0	41.0	35.0	41.0
39	38.911854805500035	40.0	39.0	41.0	35.0	41.0
40	38.814047387081544	40.0	38.0	41.0	35.0	41.0
41	38.77178269035686	40.0	38.0	41.0	35.0	41.0
42	38.701816940027925	40.0	38.0	41.0	35.0	41.0
43	37.96412321846469	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	2.0
20	4.0
21	9.0
22	15.0
23	28.0
24	62.0
25	65.0
26	121.0
27	149.0
28	191.0
29	322.0
30	435.0
31	549.0
32	758.0
33	977.0
34	1482.0
35	2434.0
36	3802.0
37	7153.0
38	18978.0
39	62025.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.18873477094905	15.92358607113084	12.277653345118168	28.610025812801943
2	21.6134507799082	18.362243001918383	31.777869288792022	28.246436929381396
3	21.56524009923365	19.017104747747656	28.676315498729448	30.741339654289245
4	15.859305163564777	15.289816498096684	32.99820214336651	35.85267619497203
5	17.38798549662023	32.33932284081436	33.21816337394414	17.054528288621277
6	35.61564034832217	32.83549109608991	14.955354900917007	16.59351365467091
7	30.60574711489208	26.90457298393982	19.647861153239656	22.841818747928446
8	28.07066882275544	30.255215290820885	19.741269347046593	21.932846539377078
9	28.319757339573936	12.42529855468397	17.1830901037534	42.07185400198869
10	19.84371704348001	22.54251077207396	29.110211624800375	28.503560559645653
11	40.098229261874394	18.795134738808596	18.13725982543716	22.969376173879855
12	23.607163303636895	23.141126723782932	25.926297921918785	27.32541205066139
13	34.97383566184225	16.96011570563362	20.43429788174322	27.631750750780913
14	24.48198628004379	19.209947470445847	22.74238421903719	33.56568203047316
15	30.176872934724745	22.972389341422016	19.87686188644376	26.973875837409476
16	26.2627682974599	23.18933740445748	21.59738055301668	28.950513745065937
17	27.557425951407648	23.036670248988077	21.205668772535983	28.200235027068288
18	26.82924379538584	20.85413255928407	22.999507849301448	29.31711579602865
19	28.73457007121119	21.302090133885077	22.381808503158805	27.581531291744927
20	29.626467663690327	20.33888090957484	22.849853861374207	27.184797565360626
21	28.811908038126617	21.391480770969135	21.29003746371644	28.506573727187813
22	29.392444984582628	22.150798991593263	20.530719243092314	27.9260367807318
23	29.048943884776474	21.666683406486346	21.310125247330834	27.974247461406343
24	28.86413627552404	21.112260578729046	21.587336661209484	28.43626648453743
25	29.029860490342795	21.21772144270462	22.31853198477346	27.43388608217912
26	28.921386458825065	22.00114500366602	21.713889697980175	27.363578839528742
27	27.976256239767782	21.356327149643946	23.335978224842563	27.33143838574571
28	26.68662053172363	21.88764902624469	22.258268633930275	29.167461808101404
29	28.550766851139482	22.541506382893242	21.669696574028503	27.238030191938773
30	27.177766841095586	20.991733877042677	23.508733163926358	28.321766117935375
31	28.094774163092715	21.88865341542541	22.390848005785283	27.625724415696595
32	26.603256229723893	21.386458825065535	22.429014794652634	29.581270150557938
33	26.811164790132878	21.235800447957576	23.92856784146721	28.024466920442332
34	26.947761718710765	21.393489549330575	23.61419402790193	28.04455470405673
35	26.63740546186836	22.66906380884465	23.948655625081607	26.744875104205377
36	26.799112119964242	21.09116840593393	24.152546628767716	27.95717284533411
37	26.317005313218765	21.20968632925886	24.622600765344554	27.850707592177816
38	26.463646133603845	20.38006086598435	25.180036760644015	27.976256239767782
39	25.451221839438347	19.591615359119352	26.7408575474825	28.216305253959806
40	25.228247441318562	20.33888090957484	27.672930707190424	26.75994094191617
41	23.39121962978215	19.71816839589004	28.219318421501967	28.67129355282585
42	21.58030593694445	20.41923204403242	29.619436939425288	28.381025079597844
43	21.543143537257816	20.62714060444141	29.159426694655643	28.67028916364513
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	3.0
4	5.0
5	5.0
6	6.5
7	8.0
8	5.5
9	3.0
10	9.5
11	16.0
12	16.0
13	31.5
14	47.0
15	70.0
16	93.0
17	88.5
18	84.0
19	84.0
20	91.0
21	98.0
22	79.5
23	61.0
24	82.5
25	104.0
26	104.0
27	132.5
28	161.0
29	213.0
30	265.0
31	369.5
32	474.0
33	474.0
34	683.0
35	892.0
36	1091.0
37	1290.0
38	1752.0
39	2214.0
40	2214.0
41	2548.5
42	2883.0
43	3495.0
44	4107.0
45	5343.0
46	6579.0
47	6579.0
48	7262.0
49	7945.0
50	9257.5
51	10570.0
52	11048.0
53	11526.0
54	11526.0
55	10906.0
56	10286.0
57	9763.5
58	9241.0
59	8590.5
60	7940.0
61	7940.0
62	7480.5
63	7021.0
64	5870.5
65	4720.0
66	4033.0
67	3346.0
68	3346.0
69	2857.0
70	2368.0
71	2066.5
72	1765.0
73	1614.0
74	1463.0
75	1463.0
76	1318.0
77	1173.0
78	806.0
79	439.0
80	320.0
81	201.0
82	201.0
83	144.5
84	88.0
85	70.5
86	53.0
87	38.5
88	24.0
89	24.0
90	15.0
91	6.0
92	4.5
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	99563.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.48219720177175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.94347469029023	48.827375631509696
2	6.685188097359822	7.685586010867492
3	2.456710524016704	4.236513564275886
4	1.3628977302510876	3.1336942438456057
5	0.933060753787283	2.6817191125217197
6	0.5241914347119567	1.8079005252955416
7	0.47701420558788066	1.9193877243554331
8	0.38265974733972846	1.7596898446209936
9	0.3162621656095473	1.636149975392465
>10	1.7857454875853995	19.530347619095448
>50	0.10134367737764498	4.024587447144019
>100	0.03145148608271741	2.757048301075701
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	220	0.22096561975834395	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	216	0.21694806303546496	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	202	0.20288661450538853	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	187	0.18782077679459236	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	179	0.17978566334883442	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	163	0.16371543645731848	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	159	0.1596978797344395	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	148	0.1486495987465223	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	148	0.1486495987465223	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	146	0.1466408203850828	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	141	0.1416188744814841	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	136	0.13659692857788536	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	131	0.13157498267428663	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	123	0.12353986922852868	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	119	0.11952231250564968	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	118	0.11851792332492994	No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	107	0.10746964233701274	RNA PCR Primer, Index 39 (95% over 23bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	102	0.10244769643341402	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0010043891807197452
2	0.0	0.0	0.0	0.0	0.0010043891807197452
3	0.0	0.0	0.0	0.0	0.0010043891807197452
4	0.0	0.0	0.0	0.0	0.0010043891807197452
5	0.0	0.0	0.0	0.0	0.0010043891807197452
6	0.0	0.0	0.0	0.0	0.0010043891807197452
7	0.0	0.0	0.0	0.0	0.0010043891807197452
8	0.0	0.0	0.0	0.0	0.0010043891807197452
9	0.0	0.0	0.0	0.0	0.0010043891807197452
10	0.0	0.0	0.0	0.0	0.0010043891807197452
11	0.0	0.0	0.0	0.0	0.0010043891807197452
12	0.0	0.0	0.0	0.0	0.0010043891807197452
13	0.0	0.0	0.0	0.0	0.0010043891807197452
14	0.0	0.0	0.0	0.0	0.0010043891807197452
15	0.0	0.0	0.0	0.0	0.0010043891807197452
16	0.0	0.0	0.0	0.0	0.0010043891807197452
17	0.0	0.0	0.0	0.0010043891807197452	0.0010043891807197452
18	0.0	0.0	0.0	0.0020087783614394904	0.0010043891807197452
19	0.0	0.0	0.0	0.0030131675421592357	0.0010043891807197452
20	0.0	0.0	0.0	0.0030131675421592357	0.0010043891807197452
21	0.0010043891807197452	0.0	0.0	0.0030131675421592357	0.0010043891807197452
22	0.0010043891807197452	0.0	0.0	0.004017556722878981	0.0010043891807197452
23	0.0010043891807197452	0.0	0.0	0.006026335084318471	0.0010043891807197452
24	0.0010043891807197452	0.0	0.0	0.007030724265038217	0.0010043891807197452
25	0.0010043891807197452	0.0	0.0	0.008035113445757962	0.0010043891807197452
26	0.0010043891807197452	0.0	0.0	0.012052670168636943	0.0010043891807197452
27	0.0010043891807197452	0.0	0.0	0.03214045378303185	0.0010043891807197452
28	0.0010043891807197452	0.0	0.0	0.1376013177586051	0.0010043891807197452
29	0.0010043891807197452	0.0	0.0	0.3173869811074395	0.0010043891807197452
30	0.0020087783614394904	0.0	0.0	0.5182648172513886	0.0010043891807197452
31	0.0020087783614394904	0.0	0.0	1.1058324879724395	0.0010043891807197452
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGGGC	30	3.5761172E-4	30.833334	4
CTCTATG	30	3.5761172E-4	30.833334	1
TCTATGG	30	3.5761172E-4	30.833334	2
TGAGTAT	25	0.00547256	29.599998	3
AATCTGA	25	0.00547256	29.599998	24
TGTACTG	25	0.00547256	29.599998	10
GCATGTA	25	0.00547256	29.599998	7
GGTATCA	105	0.0	26.428574	1
CTATGGG	35	8.812376E-4	26.42857	3
ATACGGC	35	8.812376E-4	26.42857	29
GAACTCG	45	1.3113898E-4	24.666668	27
CCGTGCC	90	1.382432E-10	24.666668	9
CAATGAG	45	1.3113898E-4	24.666668	33
GCGGTAA	85	1.9226718E-9	23.941177	23
CGTGCCA	85	1.9226718E-9	23.941177	10
CCGCGGT	85	1.9226718E-9	23.941177	21
TGAGCAA	40	0.0019188902	23.125	36
CGCGGTA	90	3.7234713E-9	22.611113	22
CGGTAAT	90	3.7234713E-9	22.611113	24
AATGAGC	50	2.6781566E-4	22.199999	34
>>END_MODULE