FastQCFastQC Report
Fri 10 Feb 2017
ERR1632403.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632403.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501683
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA20200.40264469794671137No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA14310.2852398825553188No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA12200.2431814512351425No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA11770.23461030172439568No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA10690.21308276341833388No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9840.1961397934552297No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA9630.19195388322905102No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT8980.17899749443373605No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG8940.1782001782001782No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT8670.17281829362366274No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT8050.16045989200351615No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT7880.15707129801089534No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7850.15647331083572694No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC7800.15547666554377965No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7630.1520880715511588No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7120.14192228957329628No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA7030.14012832804779113No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC7000.13953034087262275No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6900.13753705028872815No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6840.13634107593839137No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC6490.12936455889476026No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6310.12577663584374993No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC6060.12079340938401341No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5920.11800280256656094No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT5830.1162088410410558No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC5810.11581018292427687No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5640.11242158893165605No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC5580.11122561458131927No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA5400.10763769153030898No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC5360.10684037529675114No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5190.10345178130413028No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC5130.10225580695379353No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA5060.10086050354506731No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5030.10026251636989893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7350.030.4557841
TTTATAC250.00549342729.63
TGTCGTG250.00549342729.65
CGTGCAT358.8624004E-426.4285710
AGACCGT358.8624004E-426.428576
ATACGGC1000.025.90000229
CGTATGC1103.6379788E-1223.54545431
GCCGTCT1103.6379788E-1223.54545436
AACGTCA4550.022.7692328
CTCGTAT1250.022.229
CGTTAGA502.6991614E-422.21
ACCGTCC502.6991614E-422.28
ACGGGTA753.732075E-722.19999915
CGCGGTA7950.021.87421222
GCGGTAA8000.021.73750123
ATGAGCA4150.021.3975935
GGTAATA8050.021.37267125
ATGCCGT1301.8189894E-1221.34615334
TGAGCAA4200.021.14285736
ACGTCAA4850.020.97938229
Produced by FastQC (version 0.11.5)