##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632403.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	501683
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.0326241869866	34.0	33.0	34.0	31.0	34.0
2	33.1718356013658	34.0	33.0	34.0	31.0	34.0
3	33.240853287833154	34.0	34.0	34.0	31.0	34.0
4	36.530235228221805	37.0	37.0	37.0	35.0	37.0
5	36.50046543335134	37.0	37.0	37.0	35.0	37.0
6	36.54613371391895	37.0	37.0	37.0	35.0	37.0
7	36.54818480992978	37.0	37.0	37.0	35.0	37.0
8	36.52152056178902	37.0	37.0	37.0	35.0	37.0
9	38.31968992371677	39.0	39.0	39.0	37.0	39.0
10	38.34520005660945	39.0	39.0	39.0	37.0	39.0
11	38.414337340511835	39.0	39.0	39.0	37.0	39.0
12	38.36190383170249	39.0	39.0	39.0	37.0	39.0
13	38.40038231313399	39.0	39.0	39.0	37.0	39.0
14	39.933503826121274	41.0	40.0	41.0	38.0	41.0
15	39.9378073404919	41.0	40.0	41.0	38.0	41.0
16	39.900494934051984	41.0	40.0	41.0	38.0	41.0
17	39.900269692216	41.0	40.0	41.0	38.0	41.0
18	39.9094029496714	41.0	40.0	41.0	38.0	41.0
19	39.90918568099776	41.0	40.0	41.0	38.0	41.0
20	39.88889796943488	41.0	40.0	41.0	38.0	41.0
21	39.85056101163484	41.0	40.0	41.0	38.0	41.0
22	39.82019123629862	41.0	40.0	41.0	38.0	41.0
23	39.78321170938621	41.0	40.0	41.0	38.0	41.0
24	39.76033272006426	41.0	40.0	41.0	38.0	41.0
25	39.725444154974355	41.0	40.0	41.0	38.0	41.0
26	39.63691215369068	41.0	40.0	41.0	37.0	41.0
27	39.532232505386865	41.0	40.0	41.0	37.0	41.0
28	39.494210487499075	41.0	40.0	41.0	37.0	41.0
29	39.44280153004985	41.0	39.0	41.0	37.0	41.0
30	39.407657823765206	41.0	39.0	41.0	36.0	41.0
31	39.343711068543286	41.0	39.0	41.0	36.0	41.0
32	39.29842549976778	41.0	39.0	41.0	36.0	41.0
33	39.2212672145558	41.0	39.0	41.0	35.0	41.0
34	39.18580458177774	41.0	39.0	41.0	35.0	41.0
35	39.09224151506031	41.0	39.0	41.0	35.0	41.0
36	39.04069103397963	41.0	39.0	41.0	35.0	41.0
37	38.97979799993223	40.0	39.0	41.0	35.0	41.0
38	38.90702694729541	40.0	39.0	41.0	35.0	41.0
39	38.801484204168766	40.0	38.0	41.0	35.0	41.0
40	38.72805137905809	40.0	38.0	41.0	35.0	41.0
41	38.669257279995534	40.0	38.0	41.0	35.0	41.0
42	38.60304216008914	40.0	38.0	41.0	35.0	41.0
43	37.85597080227953	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	3.0
18	7.0
19	12.0
20	34.0
21	51.0
22	100.0
23	170.0
24	278.0
25	444.0
26	592.0
27	895.0
28	1175.0
29	1705.0
30	2271.0
31	2952.0
32	3822.0
33	5367.0
34	7881.0
35	12456.0
36	19926.0
37	38525.0
38	100459.0
39	302556.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.08098938971422	16.051171755869742	12.465441324501727	28.402397529914307
2	21.982207888248155	19.45092817576039	31.03473707500553	27.532126860985922
3	21.62281759597196	18.93386859829813	28.785308651080467	30.658005154649448
4	15.834102411283618	15.751978839227162	34.10360725796968	34.31031149151955
5	17.625472659029708	32.47728147057006	32.591098362910444	17.30614750748979
6	33.9044775286386	33.69697597885518	15.476107422416147	16.922439070090075
7	30.092508615998547	27.22954535035072	19.52248730772221	23.155458725928526
8	27.255458127941353	29.730726375021675	20.646902526097158	22.36691297093982
9	28.21662284749533	12.409429859094288	17.35418581056165	42.019761482848736
10	19.731184831855973	22.75440866044893	28.73069248908175	28.78371401861335
11	40.42612566102499	18.72596839039792	18.225253795723596	22.622652152853494
12	22.7681623654778	23.344024015164955	26.596276931847402	27.291536687509844
13	36.769832742987106	17.059976120378806	19.657034422135094	26.513156714498997
14	24.103268398570414	19.978552193317295	22.726502592274407	33.19167681583789
15	30.107258966319367	23.510862437036934	19.249805155845422	27.132073440798273
16	25.62355112690683	23.90055074618833	22.145259058010737	28.330639068894104
17	27.53292417721948	23.282630665181	21.20183462465342	27.982610532946104
18	27.369075691223344	20.456144617218442	22.616472952043424	29.558306739514794
19	28.591959464442684	22.145059728952347	22.59972931113871	26.663251495466262
20	30.87846309322819	20.344321015461954	21.658098839306895	27.119117052002956
21	28.035432733419313	21.36847371746701	21.239906474805803	29.356187074307883
22	28.927629598770537	22.174959087710768	20.481658736692292	28.415752576826403
23	29.274262831309812	21.072071407641875	21.31923944004481	28.3344263210035
24	28.18951409555436	20.870151071493353	22.17416177147721	28.766173061475076
25	29.056396170490128	21.472722815004694	21.983005204481714	27.48787581002346
26	28.82736708240064	22.116356344544265	21.649926347912928	27.406350225142173
27	27.160577496147965	21.48248993886578	24.053037475856268	27.303895089129988
28	27.0635042447123	22.78630130979124	21.16396210355942	28.98623234193704
29	27.390403900471018	23.581424923706802	21.55225510930209	27.475916066520096
30	27.289144738809167	21.48109463545705	23.283826639531338	27.94593398620244
31	28.775541527219378	21.964268272993106	21.741019727596907	27.519170472190606
32	26.181473161338932	21.564414181863846	22.337611599356567	29.91650105744065
33	26.557806423578235	21.25286286360112	23.949585694552137	28.239745018268508
34	26.99274242898404	21.36648042688311	23.132137226096958	28.50863991803589
35	26.00546560278104	23.189344665854733	24.08592677049053	26.719262960873696
36	26.757733469142863	21.516176549733597	24.497939934181545	27.228150046941995
37	27.072673381398214	21.172732582128557	24.199145675655743	27.555448360817486
38	26.169314088777174	20.59766824867496	25.374190474861614	27.85882718768625
39	25.503953691873154	19.6133813583478	26.380004903494836	28.502660046284205
40	25.351865620321995	20.574944736018562	27.34296358457432	26.730226059085123
41	23.44807378364425	20.029381103206607	28.199281219415447	28.323263893733692
42	21.727864009743207	20.7236442135771	29.705411584606217	27.843080192073483
43	20.91938534891555	21.312661581117958	28.99041825216322	28.777534817803275
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.5
2	3.0
3	8.5
4	14.0
5	14.0
6	18.5
7	23.0
8	28.0
9	33.0
10	47.5
11	62.0
12	62.0
13	95.0
14	128.0
15	254.5
16	381.0
17	383.5
18	386.0
19	386.0
20	473.0
21	560.0
22	535.0
23	510.0
24	628.0
25	746.0
26	746.0
27	968.0
28	1190.0
29	1484.0
30	1778.0
31	2196.0
32	2614.0
33	2614.0
34	3482.0
35	4350.0
36	5049.0
37	5748.0
38	7270.0
39	8792.0
40	8792.0
41	10984.5
42	13177.0
43	15919.5
44	18662.0
45	26956.5
46	35251.0
47	35251.0
48	39578.5
49	43906.0
50	49416.0
51	54926.0
52	56692.5
53	58459.0
54	58459.0
55	54173.0
56	49887.0
57	48780.0
58	47673.0
59	45918.5
60	44164.0
61	44164.0
62	40309.0
63	36454.0
64	29876.0
65	23298.0
66	20159.5
67	17021.0
68	17021.0
69	14573.0
70	12125.0
71	10098.5
72	8072.0
73	6334.0
74	4596.0
75	4596.0
76	3675.5
77	2755.0
78	2177.0
79	1599.0
80	1317.0
81	1035.0
82	1035.0
83	856.5
84	678.0
85	498.5
86	319.0
87	250.0
88	181.0
89	181.0
90	130.5
91	80.0
92	55.5
93	31.0
94	20.5
95	10.0
96	10.0
97	7.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	501683.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.496839091515156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.25347872631873	45.805318328704956
2	6.68243517572903	7.016134483194556
3	1.9174807669100984	3.019850378446636
4	0.9760957421996265	2.0496776446466742
5	0.5595716999447664	1.4687872746083004
6	0.39897470225687326	1.2566946447578549
7	0.2832288308518478	1.0408032851515194
8	0.20507788165592475	0.8612752443615898
9	0.18166137390130782	0.8582983123356475
>10	1.2565043699946563	13.339196130884387
>50	0.15721022879147692	5.796112049439468
>100	0.1153382550089872	12.129570945028856
>500	0.01103897490185026	3.9760469380589325
>1k	0.0019032715348017688	1.382234340380642
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2020	0.40264469794671137	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1431	0.2852398825553188	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1220	0.2431814512351425	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1177	0.23461030172439568	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1069	0.21308276341833388	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	984	0.1961397934552297	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	963	0.19195388322905102	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	898	0.17899749443373605	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	894	0.1782001782001782	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	867	0.17281829362366274	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	805	0.16045989200351615	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	788	0.15707129801089534	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	785	0.15647331083572694	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	780	0.15547666554377965	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	763	0.1520880715511588	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	712	0.14192228957329628	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	703	0.14012832804779113	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	700	0.13953034087262275	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	690	0.13753705028872815	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	684	0.13634107593839137	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	649	0.12936455889476026	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	631	0.12577663584374993	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	606	0.12079340938401341	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	592	0.11800280256656094	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	583	0.1162088410410558	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	581	0.11581018292427687	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	564	0.11242158893165605	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	558	0.11122561458131927	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	540	0.10763769153030898	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	536	0.10684037529675114	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	519	0.10345178130413028	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	513	0.10225580695379353	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	506	0.10086050354506731	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	503	0.10026251636989893	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9932905838946106E-4
2	0.0	0.0	0.0	0.0	1.9932905838946106E-4
3	0.0	0.0	0.0	0.0	1.9932905838946106E-4
4	0.0	0.0	0.0	0.0	1.9932905838946106E-4
5	0.0	0.0	0.0	0.0	1.9932905838946106E-4
6	0.0	0.0	0.0	0.0	1.9932905838946106E-4
7	0.0	0.0	0.0	0.0	1.9932905838946106E-4
8	0.0	0.0	0.0	0.0	1.9932905838946106E-4
9	0.0	0.0	0.0	0.0	1.9932905838946106E-4
10	0.0	0.0	0.0	0.0	1.9932905838946106E-4
11	0.0	0.0	0.0	0.0	1.9932905838946106E-4
12	0.0	0.0	0.0	0.0	1.9932905838946106E-4
13	0.0	0.0	0.0	0.0	1.9932905838946106E-4
14	0.0	0.0	0.0	0.0	1.9932905838946106E-4
15	0.0	0.0	0.0	0.0	1.9932905838946106E-4
16	0.0	0.0	0.0	3.986581167789221E-4	1.9932905838946106E-4
17	0.0	0.0	0.0	7.973162335578442E-4	1.9932905838946106E-4
18	0.0	0.0	0.0	0.0011959743503367664	1.9932905838946106E-4
19	0.0	0.0	0.0	0.0017939615255051498	1.9932905838946106E-4
20	0.0	0.0	0.0	0.002591277759062994	1.9932905838946106E-4
21	0.0	0.0	0.0	0.0037872521093997606	1.9932905838946106E-4
22	0.0	0.0	0.0	0.006378529868462754	1.9932905838946106E-4
23	0.0	0.0	0.0	0.007973162335578442	1.9932905838946106E-4
24	0.0	0.0	0.0	0.009567794802694131	1.9932905838946106E-4
25	0.0	0.0	0.0	0.010564440094641437	1.9932905838946106E-4
26	0.0	0.0	0.0	0.014152363145651737	1.9932905838946106E-4
27	0.0	0.0	0.0	0.04385239284568144	1.9932905838946106E-4
28	0.0	0.0	0.0	0.15129075531760094	1.9932905838946106E-4
29	0.0	0.0	0.0	0.307365408036549	1.9932905838946106E-4
30	0.0	0.0	0.0	0.52523206885623	1.9932905838946106E-4
31	0.0	0.0	0.0	1.0524574282963546	1.9932905838946106E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	735	0.0	30.455784	1
TTTATAC	25	0.005493427	29.6	3
TGTCGTG	25	0.005493427	29.6	5
CGTGCAT	35	8.8624004E-4	26.42857	10
AGACCGT	35	8.8624004E-4	26.42857	6
ATACGGC	100	0.0	25.900002	29
CGTATGC	110	3.6379788E-12	23.545454	31
GCCGTCT	110	3.6379788E-12	23.545454	36
AACGTCA	455	0.0	22.76923	28
CTCGTAT	125	0.0	22.2	29
CGTTAGA	50	2.6991614E-4	22.2	1
ACCGTCC	50	2.6991614E-4	22.2	8
ACGGGTA	75	3.732075E-7	22.199999	15
CGCGGTA	795	0.0	21.874212	22
GCGGTAA	800	0.0	21.737501	23
ATGAGCA	415	0.0	21.39759	35
GGTAATA	805	0.0	21.372671	25
ATGCCGT	130	1.8189894E-12	21.346153	34
TGAGCAA	420	0.0	21.142857	36
ACGTCAA	485	0.0	20.979382	29
>>END_MODULE