FastQCFastQC Report
Fri 10 Feb 2017
ERR1632402.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632402.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134712
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5400.4008551576696953No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3820.2835679078330067No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2950.21898568798622248No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2900.2152740661559475No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2880.2137894174238375No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2640.19597363263851772No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2510.18632341587980283No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2430.1803848209513629No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2380.17667319912108795No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2320.172219252924758No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC2210.1640536848981531No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2200.1633113605320981No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2130.15811508996971316No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2100.1558881168715482No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2070.15366114377338322No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2050.15217649504127323No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2010.14920719757705328No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1970.1462379001128333No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1960.14549557574677832No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1930.14326860264861335No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1840.13658768335411842No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1780.13213373715778848No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1770.13139141279173347No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1770.13139141279173347No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1720.1276797909614585No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1680.12471049349723855No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC1640.12174119603301858No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1600.11877189856879862No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1570.11654492547063366No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1540.11431795237246867No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1510.1120909792743037No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1460.10837935744402874No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1420.10541005997980878No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1390.10318308688164382No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1380.10244076251558881No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1360.10095611378347882No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1360.10095611378347882No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAGA200.00183617437.02
GGACTGG200.00183617437.06
TACGGCT601.2496457E-930.83333430
GAGGGCT303.58278E-430.83333434
GGTATCA2500.030.341
CATCTAA453.9738243E-628.77777931
ACATCTA453.9738243E-628.77777930
ATACGGC706.4828782E-926.4285729
AACATCT601.32282E-624.66666829
AAACATC601.32282E-624.66666828
ATCTAAG551.8865436E-523.54545432
TGGACTG400.001922397523.1255
AGTGTCA400.001922397523.1255
GAAACAT652.6537691E-622.7692327
CTGGACT502.6849812E-422.1999994
GGTAATA2650.021.64150825
GGGGAAC705.0469753E-621.14285719
GCGGTAA2800.021.14285723
GTATCAA3600.021.0694452
CGCGGTA2850.020.7719322
Produced by FastQC (version 0.11.5)