##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632402.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	134712
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.010845358988064	34.0	33.0	34.0	31.0	34.0
2	33.148271868875824	34.0	33.0	34.0	31.0	34.0
3	33.225080171031536	34.0	33.0	34.0	31.0	34.0
4	36.522529544509766	37.0	37.0	37.0	35.0	37.0
5	36.48971880753014	37.0	37.0	37.0	35.0	37.0
6	36.5356389928143	37.0	37.0	37.0	35.0	37.0
7	36.542854385652355	37.0	37.0	37.0	35.0	37.0
8	36.51975325138072	37.0	37.0	37.0	35.0	37.0
9	38.31875408278401	39.0	39.0	39.0	37.0	39.0
10	38.34022210345032	39.0	39.0	39.0	37.0	39.0
11	38.41207167884079	39.0	39.0	39.0	37.0	39.0
12	38.35718421521468	39.0	39.0	39.0	37.0	39.0
13	38.400031177623376	39.0	39.0	39.0	37.0	39.0
14	39.92475057901301	41.0	40.0	41.0	38.0	41.0
15	39.93156511669339	41.0	40.0	41.0	38.0	41.0
16	39.889260051071915	41.0	40.0	41.0	38.0	41.0
17	39.88686234336956	41.0	40.0	41.0	38.0	41.0
18	39.894419205416	41.0	40.0	41.0	38.0	41.0
19	39.90148613338084	41.0	40.0	41.0	38.0	41.0
20	39.88548904329236	41.0	40.0	41.0	38.0	41.0
21	39.85488300967991	41.0	40.0	41.0	38.0	41.0
22	39.80899251737039	41.0	40.0	41.0	38.0	41.0
23	39.76300552289328	41.0	40.0	41.0	38.0	41.0
24	39.751833541184155	41.0	40.0	41.0	38.0	41.0
25	39.717642080883664	41.0	40.0	41.0	38.0	41.0
26	39.62785052556565	41.0	40.0	41.0	37.0	41.0
27	39.519278163786446	41.0	40.0	41.0	37.0	41.0
28	39.47994981887285	41.0	39.0	41.0	37.0	41.0
29	39.46457628125186	41.0	39.0	41.0	37.0	41.0
30	39.41662955044836	41.0	39.0	41.0	37.0	41.0
31	39.360621177029515	41.0	39.0	41.0	36.0	41.0
32	39.31360977492725	41.0	39.0	41.0	36.0	41.0
33	39.24086198705386	41.0	39.0	41.0	36.0	41.0
34	39.212289922204405	41.0	39.0	41.0	35.0	41.0
35	39.11877932181246	41.0	39.0	41.0	35.0	41.0
36	39.07686026486133	41.0	39.0	41.0	35.0	41.0
37	39.00712631391413	41.0	39.0	41.0	35.0	41.0
38	38.94339776708831	40.0	39.0	41.0	35.0	41.0
39	38.82686026486133	40.0	39.0	41.0	35.0	41.0
40	38.77025803194964	40.0	38.0	41.0	35.0	41.0
41	38.72184363679553	40.0	38.0	41.0	35.0	41.0
42	38.64456767028921	40.0	38.0	41.0	35.0	41.0
43	37.93319080705505	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	7.0
20	13.0
21	17.0
22	38.0
23	52.0
24	80.0
25	137.0
26	177.0
27	227.0
28	307.0
29	431.0
30	592.0
31	794.0
32	1088.0
33	1372.0
34	2101.0
35	3304.0
36	5212.0
37	9996.0
38	27340.0
39	81425.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.56116752776293	16.320001187718987	12.5890789239266	28.52975236059148
2	21.763465764000237	19.586970722727003	31.446344794821545	27.203218718451218
3	21.38339568858008	19.115594750282085	29.180028505255656	30.32098105588218
4	15.662301799394262	15.947354355959382	34.607161945483696	33.783181899162656
5	17.37335946315102	32.4959914484233	32.99186412494804	17.13878496347764
6	33.140328998159035	33.979155531801176	15.962943167646534	16.917572302393253
7	29.450976898865726	27.39399608052735	19.775521111704972	23.379505908901955
8	26.81943702120078	30.179197101965677	20.908308094304886	22.093057782528653
9	27.72507274778787	12.499257675633944	17.47060395510422	42.30506562147396
10	19.5491121800582	22.691371221568975	28.998159035572186	28.761357562800644
11	39.92814300136588	18.969356850169248	18.552170556446345	22.550329592018528
12	22.46867391175248	23.6838588989845	26.75930874755033	27.08815844171269
13	36.451837995130354	17.011847496882236	20.20829621711503	26.32801829087238
14	24.112922382564285	20.1444563216343	22.606746243838707	33.13587505196271
15	29.957984440881287	23.74769879446523	19.192054159985748	27.102262604667736
16	25.490676405962347	23.84494328641843	22.521378941742384	28.143001365876835
17	27.333867806876892	23.45744996733773	21.234930815369083	27.9737514104163
18	26.527703545341176	20.70491121800582	23.06550270206069	29.701882534592315
19	28.262515588811688	22.39147217768276	22.894025773501987	26.451986460003564
20	30.875497357325255	20.26916681513154	21.98987469564701	26.865461131896197
21	28.167498069956647	21.382653364214026	21.436100718569985	29.013747847259335
22	28.60472712156304	22.34247876952313	20.803640358691133	28.2491537502227
23	28.8148049171566	21.422738879980997	21.763465764000237	27.998990438862165
24	27.90248827127502	21.16292535186175	22.470900884850646	28.46368549201259
25	28.943227032484113	21.79241641427638	22.17248648969654	27.091870063542967
26	28.667824692677712	22.41819585486074	21.865164202149774	27.048815250311776
27	27.050299899043885	21.649890135993825	24.25544866084684	27.044361304115444
28	26.937466595403524	23.113011461488213	21.174060217352576	28.775461725755687
29	27.108201199596177	23.883544153453293	21.649890135993825	27.358364510956708
30	27.252954450976898	21.891145554961696	23.610368786745056	27.245531207316347
31	28.384999109210764	22.130916325197457	22.070045727180947	27.414038838410832
32	25.648049171566008	21.63281667557456	22.703248411425854	30.01588574143358
33	26.3161411010155	21.738969059920425	24.023843458637685	27.92104638042639
34	26.44159391887879	21.759754142169964	23.63635013955698	28.162301799394264
35	25.897470158560488	23.2154522240038	24.248025417186295	26.63905220024942
36	26.25972444919532	21.636528297404833	25.01039254112477	27.093354712275076
37	27.05623849397233	21.091662212720472	24.77359106835323	27.078508224953975
38	25.7735019894293	20.484440881287487	26.100867034859554	27.641190094423656
39	25.1714769285587	19.724300730447176	26.98942930102738	28.114793039966745
40	24.674861927667912	20.430251202565472	28.361987053863057	26.532899815903555
41	22.99349723855336	19.67011105172516	29.051606389928143	28.284785319793336
42	21.42348120434705	20.491121800581983	30.58747550329592	27.497921491775045
43	20.491121800581983	21.289862818457152	29.576429716729024	28.642585664231845
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	3.0
2	2.0
3	3.5
4	5.0
5	5.0
6	4.5
7	4.0
8	6.5
9	9.0
10	13.0
11	17.0
12	17.0
13	29.0
14	41.0
15	89.5
16	138.0
17	123.0
18	108.0
19	108.0
20	144.0
21	180.0
22	153.5
23	127.0
24	180.5
25	234.0
26	234.0
27	276.5
28	319.0
29	424.0
30	529.0
31	644.0
32	759.0
33	759.0
34	992.0
35	1225.0
36	1416.0
37	1607.0
38	2053.0
39	2499.0
40	2499.0
41	3073.0
42	3647.0
43	4377.5
44	5108.0
45	7332.0
46	9556.0
47	9556.0
48	10984.0
49	12412.0
50	13915.5
51	15419.0
52	15879.5
53	16340.0
54	16340.0
55	15307.0
56	14274.0
57	13561.5
58	12849.0
59	12028.5
60	11208.0
61	11208.0
62	10300.5
63	9393.0
64	7487.5
65	5582.0
66	4802.0
67	4022.0
68	4022.0
69	3389.0
70	2756.0
71	2325.0
72	1894.0
73	1469.5
74	1045.0
75	1045.0
76	842.0
77	639.0
78	496.0
79	353.0
80	273.0
81	193.0
82	193.0
83	149.5
84	106.0
85	83.5
86	61.0
87	40.5
88	20.0
89	20.0
90	20.5
91	21.0
92	13.0
93	5.0
94	3.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	134712.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.813053031652714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.98748268743304	49.42024467011105
2	5.798729976219719	6.588871073104103
3	2.078814644471738	3.543114199180474
4	1.1681082917396188	2.654551933012649
5	0.7983379935714846	2.2678009382979987
6	0.5121906603601014	1.7459469089613397
7	0.367157080513236	1.460152028030168
8	0.3253455980348603	1.4787101371815428
9	0.23649619776831213	1.209246392303581
>10	1.4934538897744791	15.777362076132787
>50	0.14895340632921317	5.735198052140864
>100	0.08362296495675124	7.717946433873745
>500	0.0013066088274492382	0.4008551576696953
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	540	0.4008551576696953	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	382	0.2835679078330067	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	295	0.21898568798622248	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	290	0.2152740661559475	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	288	0.2137894174238375	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	264	0.19597363263851772	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	251	0.18632341587980283	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	243	0.1803848209513629	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	238	0.17667319912108795	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	232	0.172219252924758	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	221	0.1640536848981531	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	220	0.1633113605320981	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	213	0.15811508996971316	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	210	0.1558881168715482	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	207	0.15366114377338322	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	205	0.15217649504127323	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	201	0.14920719757705328	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	197	0.1462379001128333	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	196	0.14549557574677832	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	193	0.14326860264861335	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	184	0.13658768335411842	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	178	0.13213373715778848	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	177	0.13139141279173347	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	177	0.13139141279173347	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	172	0.1276797909614585	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	168	0.12471049349723855	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	164	0.12174119603301858	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	160	0.11877189856879862	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	157	0.11654492547063366	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	154	0.11431795237246867	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	151	0.1120909792743037	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	146	0.10837935744402874	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	142	0.10541005997980878	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	139	0.10318308688164382	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	138	0.10244076251558881	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	136	0.10095611378347882	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	136	0.10095611378347882	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.002226973098164974	0.0
18	0.0	0.0	0.0	0.002226973098164974	0.0
19	0.0	0.0	0.0	0.002226973098164974	0.0
20	0.0	0.0	0.0	0.003711621830274957	0.0
21	0.0	0.0	0.0	0.00519627056238494	0.0
22	0.0	0.0	0.0	0.007423243660549914	0.0
23	0.0	0.0	0.0	0.009650216758714887	0.0
24	0.0	0.0	0.0	0.011877189856879862	0.0
25	0.0	0.0	0.0	0.014104162955044837	0.0
26	0.0	0.0	0.0	0.018558109151374784	0.0
27	0.0	0.0	0.0	0.046024110695409466	0.0
28	0.0	0.0	0.0	0.1803848209513629	0.0
29	0.0	0.0	0.0	0.3771007779559356	0.0
30	0.0	0.0	0.0	0.6235524674861928	0.0
31	0.0	0.0	0.0	1.2574974760971553	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTAAGA	20	0.001836174	37.0	2
GGACTGG	20	0.001836174	37.0	6
TACGGCT	60	1.2496457E-9	30.833334	30
GAGGGCT	30	3.58278E-4	30.833334	34
GGTATCA	250	0.0	30.34	1
CATCTAA	45	3.9738243E-6	28.777779	31
ACATCTA	45	3.9738243E-6	28.777779	30
ATACGGC	70	6.4828782E-9	26.42857	29
AACATCT	60	1.32282E-6	24.666668	29
AAACATC	60	1.32282E-6	24.666668	28
ATCTAAG	55	1.8865436E-5	23.545454	32
TGGACTG	40	0.0019223975	23.125	5
AGTGTCA	40	0.0019223975	23.125	5
GAAACAT	65	2.6537691E-6	22.76923	27
CTGGACT	50	2.6849812E-4	22.199999	4
GGTAATA	265	0.0	21.641508	25
GGGGAAC	70	5.0469753E-6	21.142857	19
GCGGTAA	280	0.0	21.142857	23
GTATCAA	360	0.0	21.069445	2
CGCGGTA	285	0.0	20.77193	22
>>END_MODULE