FastQCFastQC Report
Fri 10 Feb 2017
ERR1632401.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632401.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785156
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA17370.2212299211876366No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA13970.17792642481239396No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA13010.16569955524761956No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA11960.1523264166611476No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC10800.13755228260371188No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA10490.1336040226400868No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG9580.12201396919847775No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA9440.12023088405361483No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT9070.11551844474219136No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT8790.1119522744524655No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC8660.11029655253223564No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8510.10838610416273964No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT8300.10571147644544522No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT8240.10494729709764683No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA7980.10163585325718712No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7970.10150849003255404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9650.028.9481871
GTCGAAT400.001930644923.12500229
ATACGGC1600.023.12500229
ATGCTAG705.0985236E-621.14285915
GTATCAA13350.020.9250932
AACGACC1251.8189894E-1120.7237
ATTATCC450.003824745320.5555553
TCGAATC450.003824745320.55555530
TACGGCT1950.018.9743630
GCGGTAA7950.018.84905823
CGTGCCA8250.018.61212210
AAATCCT609.2332606E-418.55
GTTTAGG701.2185727E-418.51
TAAACCG500.007032944418.55
TATTGCA801.6156071E-518.52
TAACCCT902.1504493E-618.535
TAACACC701.2185727E-418.535
CCATACA500.007032944418.52
GTCAAGG902.1504493E-618.51
TATACAC3000.018.537
Produced by FastQC (version 0.11.5)