##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632401.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	785156
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.04226293882999	34.0	33.0	34.0	31.0	34.0
2	33.184709790156354	34.0	33.0	34.0	31.0	34.0
3	33.25535817086031	34.0	34.0	34.0	31.0	34.0
4	36.543024570913296	37.0	37.0	37.0	35.0	37.0
5	36.50888867944714	37.0	37.0	37.0	35.0	37.0
6	36.55464009699983	37.0	37.0	37.0	35.0	37.0
7	36.55031484189129	37.0	37.0	37.0	35.0	37.0
8	36.5257885057237	37.0	37.0	37.0	35.0	37.0
9	38.31905251949931	39.0	39.0	39.0	37.0	39.0
10	38.34908222060329	39.0	39.0	39.0	37.0	39.0
11	38.41957012364422	39.0	39.0	39.0	37.0	39.0
12	38.37979076769457	39.0	39.0	39.0	37.0	39.0
13	38.40934795123517	39.0	39.0	39.0	37.0	39.0
14	39.96262144083469	41.0	40.0	41.0	38.0	41.0
15	39.95737534961205	41.0	40.0	41.0	38.0	41.0
16	39.923112094921265	41.0	40.0	41.0	38.0	41.0
17	39.91917784491235	41.0	40.0	41.0	38.0	41.0
18	39.91743424236712	41.0	40.0	41.0	38.0	41.0
19	39.92040944729455	41.0	40.0	41.0	38.0	41.0
20	39.89151073162531	41.0	40.0	41.0	38.0	41.0
21	39.862070722251374	41.0	40.0	41.0	38.0	41.0
22	39.831986254960796	41.0	40.0	41.0	38.0	41.0
23	39.79372379501653	41.0	40.0	41.0	38.0	41.0
24	39.77691057573272	41.0	40.0	41.0	38.0	41.0
25	39.724684011839685	41.0	40.0	41.0	38.0	41.0
26	39.64720004686966	41.0	40.0	41.0	37.0	41.0
27	39.564212716963254	41.0	40.0	41.0	37.0	41.0
28	39.51664764709179	41.0	40.0	41.0	37.0	41.0
29	39.47018554274565	41.0	40.0	41.0	37.0	41.0
30	39.42737366841749	41.0	39.0	41.0	37.0	41.0
31	39.363799041209646	41.0	39.0	41.0	36.0	41.0
32	39.309602168231535	41.0	39.0	41.0	36.0	41.0
33	39.244976794420474	41.0	39.0	41.0	36.0	41.0
34	39.20983473347972	41.0	39.0	41.0	35.0	41.0
35	39.130278568844915	41.0	39.0	41.0	35.0	41.0
36	39.07917789585764	41.0	39.0	41.0	35.0	41.0
37	39.03215029879412	41.0	39.0	41.0	35.0	41.0
38	38.953125238806045	40.0	39.0	41.0	35.0	41.0
39	38.86965647591052	40.0	39.0	41.0	35.0	41.0
40	38.793764551248415	40.0	38.0	41.0	35.0	41.0
41	38.7447220679712	40.0	38.0	41.0	35.0	41.0
42	38.68255098349882	40.0	38.0	41.0	35.0	41.0
43	37.94097223991156	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	0.0
14	0.0
15	1.0
16	0.0
17	7.0
18	13.0
19	29.0
20	39.0
21	93.0
22	155.0
23	295.0
24	452.0
25	679.0
26	955.0
27	1412.0
28	1854.0
29	2519.0
30	3369.0
31	4385.0
32	5882.0
33	8073.0
34	12096.0
35	19242.0
36	30329.0
37	58489.0
38	154613.0
39	480173.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.204789876152	16.436224138897238	12.375120358247278	27.983865626703487
2	21.446566032737444	19.099516529199292	31.708348404648245	27.745569033415013
3	21.170060472059056	19.832491886962593	28.68640117377948	30.311046467198878
4	15.965107570979525	15.239774006694212	33.42380367723102	35.37131474509525
5	17.256698031983454	32.797686064934865	33.14092995532098	16.8046859477607
6	35.57394963548645	33.21823943267325	14.751208677001767	16.45660225483853
7	30.63607741646246	27.34832313578448	19.85821925833847	22.15738018941459
8	27.97724273902256	30.505020658315036	19.88318245036655	21.63455415229585
9	28.28788164390261	12.749058785769963	17.229697028361244	41.73336254196619
10	19.459699728461604	23.144572543545486	29.75306818008141	27.642659547911496
11	39.85360870960675	19.06321801017887	18.46932329371488	22.6138499864995
12	23.459796524512328	23.579772682116676	26.1127724936191	26.8476582997519
13	35.185237073906336	17.503018508423803	20.33341144944444	26.97833296822542
14	24.469277442954013	19.376913632450112	22.465471829801974	33.6883370947939
15	29.578325835884844	23.892576761815487	19.683094824468004	26.84600257783167
16	26.416151694695067	23.5043736531339	21.895139309895104	28.18433534227593
17	27.218157920209485	23.159983493726088	21.558518307189907	28.063340278874517
18	26.890197616779343	21.278956029120327	23.2659496966208	28.564896657479533
19	28.58260014570353	21.894502493771938	22.701221158597782	26.821676201926753
20	29.883106032431773	20.74695983982801	22.345750398646892	27.024183729093327
21	27.85166259953436	21.884822888699826	21.55482477367555	28.708689738090264
22	28.735690741712478	22.532082796285067	20.860949925874603	27.871276536127855
23	28.405183173789666	21.928763201198233	21.91666369485809	27.749389930154006
24	28.17185374626189	21.5469282537483	21.891573139605377	28.389644860384433
25	28.392064761652463	21.88966269123588	22.32817427364753	27.390098273464126
26	28.280494576873892	22.361670801726028	21.7845880309136	27.573246590486477
27	27.398886335963805	21.735553189429872	23.22239147379629	27.64316900081003
28	26.543387556103497	22.54660220389324	22.01053039141266	28.899479848590598
29	27.635145117658144	23.114769548981347	21.95729256351604	27.292792769844464
30	27.251527085063348	21.966335352464988	23.132345673980712	27.64979188849095
31	27.974440748080635	22.13458217220527	22.382813097015116	27.50816398269898
32	25.882754509931782	22.001105512789813	22.57194748559522	29.54419249168318
33	26.38673078980483	21.744468615154187	23.941866329748482	27.926934265292502
34	26.68756272638813	21.387342133283067	23.701531924865886	28.223563215462917
35	26.219757602310878	22.854693844280625	24.122212655828907	26.80333589757959
36	26.41793477983993	21.707405916785962	24.486598841504108	27.388060461869994
37	26.567331842334518	21.369766008283705	24.36127342846517	27.70162872091661
38	25.719984308850723	20.76746531899393	25.653118615918363	27.85943175623698
39	25.087498535322915	19.817080936781988	26.813015502651705	28.28240502524339
40	24.87072632699744	20.586609540014976	27.713218774358218	26.829445358629368
41	23.431903978317685	19.890696880619902	28.49433743103281	28.183061710029598
42	21.872850745584316	20.60291203276801	29.60609101885485	27.91814620279282
43	21.21884058709352	20.7511628262409	29.380148658355793	28.64984792830979
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	3.5
2	3.0
3	13.5
4	24.0
5	24.0
6	25.0
7	26.0
8	33.0
9	40.0
10	54.5
11	69.0
12	69.0
13	131.0
14	193.0
15	336.5
16	480.0
17	489.0
18	498.0
19	498.0
20	638.0
21	778.0
22	839.5
23	901.0
24	1135.0
25	1369.0
26	1369.0
27	1788.5
28	2208.0
29	3133.5
30	4059.0
31	4937.0
32	5815.0
33	5815.0
34	7522.5
35	9230.0
36	10769.5
37	12309.0
38	15510.0
39	18711.0
40	18711.0
41	22229.5
42	25748.0
43	29609.5
44	33471.0
45	42837.0
46	52203.0
47	52203.0
48	58528.5
49	64854.0
50	72490.0
51	80126.0
52	83613.0
53	87100.0
54	87100.0
55	82552.5
56	78005.0
57	75043.0
58	72081.0
59	68044.0
60	64007.0
61	64007.0
62	58621.5
63	53236.0
64	45201.0
65	37166.0
66	32192.0
67	27218.0
68	27218.0
69	23199.0
70	19180.0
71	16417.0
72	13654.0
73	10859.5
74	8065.0
75	8065.0
76	6488.0
77	4911.0
78	3961.5
79	3012.0
80	2487.5
81	1963.0
82	1963.0
83	1546.5
84	1130.0
85	880.5
86	631.0
87	490.0
88	349.0
89	349.0
90	277.5
91	206.0
92	146.0
93	86.0
94	55.0
95	24.0
96	24.0
97	16.0
98	8.0
99	6.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	785156.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.808770041363935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.55822412680133	44.84475128974291
2	6.650049243888429	6.890617440807236
3	2.0191372189451653	3.138270475748676
4	1.056520921026757	2.1894819776546113
5	0.660799392721618	1.7117601890493623
6	0.4679954014125474	1.4547759679319086
7	0.3426572182187708	1.2426854315196831
8	0.25523623983508875	1.057878052467082
9	0.2093703377558816	0.9762497714049452
>10	1.4825777060528418	15.322086503761367
>50	0.17313230167950885	6.1831346914678145
>100	0.11468144766447819	11.1900894944934
>500	0.008138683382568884	2.8066901793261065
>1k	0.0014797606150125243	0.9915285346249016
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1737	0.2212299211876366	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1397	0.17792642481239396	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1301	0.16569955524761956	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1196	0.1523264166611476	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1080	0.13755228260371188	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1049	0.1336040226400868	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	958	0.12201396919847775	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	944	0.12023088405361483	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	907	0.11551844474219136	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	879	0.1119522744524655	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	866	0.11029655253223564	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	851	0.10838610416273964	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	830	0.10571147644544522	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	824	0.10494729709764683	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	798	0.10163585325718712	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	797	0.10150849003255404	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.2736322463306656E-4	0.0
3	0.0	0.0	0.0	1.2736322463306656E-4	0.0
4	0.0	0.0	0.0	1.2736322463306656E-4	0.0
5	1.2736322463306656E-4	0.0	0.0	1.2736322463306656E-4	0.0
6	1.2736322463306656E-4	0.0	0.0	1.2736322463306656E-4	0.0
7	1.2736322463306656E-4	0.0	0.0	1.2736322463306656E-4	0.0
8	1.2736322463306656E-4	0.0	0.0	1.2736322463306656E-4	0.0
9	1.2736322463306656E-4	0.0	0.0	1.2736322463306656E-4	0.0
10	1.2736322463306656E-4	0.0	0.0	1.2736322463306656E-4	0.0
11	2.547264492661331E-4	0.0	0.0	1.2736322463306656E-4	0.0
12	2.547264492661331E-4	0.0	0.0	1.2736322463306656E-4	0.0
13	2.547264492661331E-4	0.0	0.0	1.2736322463306656E-4	0.0
14	2.547264492661331E-4	0.0	0.0	2.547264492661331E-4	0.0
15	2.547264492661331E-4	0.0	0.0	2.547264492661331E-4	0.0
16	2.547264492661331E-4	0.0	0.0	3.8208967389919963E-4	0.0
17	2.547264492661331E-4	0.0	0.0	0.0010189057970645325	0.0
18	2.547264492661331E-4	0.0	0.0	0.0012736322463306655	0.0
19	2.547264492661331E-4	0.0	0.0	0.002037811594129065	1.2736322463306656E-4
20	3.8208967389919963E-4	0.0	0.0	0.0024199012680282644	1.2736322463306656E-4
21	3.8208967389919963E-4	0.0	0.0	0.004457712862157329	1.2736322463306656E-4
22	3.8208967389919963E-4	0.0	0.0	0.006495524456286394	1.2736322463306656E-4
23	3.8208967389919963E-4	0.0	0.0	0.008023883151883193	1.2736322463306656E-4
24	3.8208967389919963E-4	0.0	0.0	0.009934331521379191	1.2736322463306656E-4
25	3.8208967389919963E-4	0.0	0.0	0.01069851086917759	1.2736322463306656E-4
26	3.8208967389919963E-4	0.0	0.0	0.013882591485004253	1.2736322463306656E-4
27	3.8208967389919963E-4	0.0	0.0	0.041520411230379695	1.2736322463306656E-4
28	3.8208967389919963E-4	0.0	0.0	0.1450667128570628	1.2736322463306656E-4
29	5.094528985322663E-4	0.0	0.0	0.30414338042376293	1.2736322463306656E-4
30	5.094528985322663E-4	0.0	0.0	0.5105991675539638	1.2736322463306656E-4
31	5.094528985322663E-4	0.0	0.0	1.0326610253249036	1.2736322463306656E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	965	0.0	28.948187	1
GTCGAAT	40	0.0019306449	23.125002	29
ATACGGC	160	0.0	23.125002	29
ATGCTAG	70	5.0985236E-6	21.142859	15
GTATCAA	1335	0.0	20.925093	2
AACGACC	125	1.8189894E-11	20.72	37
ATTATCC	45	0.0038247453	20.555555	3
TCGAATC	45	0.0038247453	20.555555	30
TACGGCT	195	0.0	18.97436	30
GCGGTAA	795	0.0	18.849058	23
CGTGCCA	825	0.0	18.612122	10
AAATCCT	60	9.2332606E-4	18.5	5
GTTTAGG	70	1.2185727E-4	18.5	1
TAAACCG	50	0.0070329444	18.5	5
TATTGCA	80	1.6156071E-5	18.5	2
TAACCCT	90	2.1504493E-6	18.5	35
TAACACC	70	1.2185727E-4	18.5	35
CCATACA	50	0.0070329444	18.5	2
GTCAAGG	90	2.1504493E-6	18.5	1
TATACAC	300	0.0	18.5	37
>>END_MODULE