FastQCFastQC Report
Fri 10 Feb 2017
ERR1632400.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632400.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9180
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT560.6100217864923747RNA PCR Primer, Index 46 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA540.5882352941176471No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA450.49019607843137253No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA330.35947712418300654No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT270.29411764705882354No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC270.29411764705882354No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT270.29411764705882354No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA250.27233115468409586No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG250.27233115468409586No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT240.261437908496732No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC240.261437908496732No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA240.261437908496732No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA240.261437908496732No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC230.25054466230936817No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG230.25054466230936817No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC230.25054466230936817No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC220.23965141612200436No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC220.23965141612200436No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA220.23965141612200436No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC210.22875816993464054No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA210.22875816993464054No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC200.2178649237472767No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC200.2178649237472767No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC200.2178649237472767No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC190.20697167755991283No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA190.20697167755991283No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC190.20697167755991283No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA190.20697167755991283No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA180.19607843137254902No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA180.19607843137254902No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC180.19607843137254902No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC180.19607843137254902No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA180.19607843137254902No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA180.19607843137254902No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT180.19607843137254902No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC160.17429193899782133No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC160.17429193899782133No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT160.17429193899782133No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT160.17429193899782133No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT150.16339869281045752No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG150.16339869281045752No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT150.16339869281045752No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG150.16339869281045752No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT140.15250544662309368No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC140.15250544662309368No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA140.15250544662309368No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG140.15250544662309368No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC140.15250544662309368No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA140.15250544662309368No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG140.15250544662309368No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA140.15250544662309368No Hit
CCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGCCT130.14161220043572986No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT130.14161220043572986No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC130.14161220043572986No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA130.14161220043572986No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA130.14161220043572986No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA120.130718954248366No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC120.130718954248366RNA PCR Primer, Index 46 (95% over 22bp)
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC120.130718954248366No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC120.130718954248366No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG120.130718954248366No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA120.130718954248366No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG120.130718954248366No Hit
ACGTCAATGAGCAAAGGTATTAACTTTACTCCCTTCCTCCCCG120.130718954248366No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA120.130718954248366No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG120.130718954248366No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG120.130718954248366No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA110.11982570806100218No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC110.11982570806100218No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG110.11982570806100218No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA110.11982570806100218No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA110.11982570806100218No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA110.11982570806100218No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA110.11982570806100218No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC110.11982570806100218No Hit
CCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTC110.11982570806100218No Hit
ACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA110.11982570806100218No Hit
TCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTG100.10893246187363835No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG100.10893246187363835No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT100.10893246187363835No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG100.10893246187363835No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG100.10893246187363835No Hit
GACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCA100.10893246187363835No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG100.10893246187363835No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC100.10893246187363835No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAA100.10893246187363835No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT100.10893246187363835No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT100.10893246187363835No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG100.10893246187363835No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA100.10893246187363835No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG100.10893246187363835No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT100.10893246187363835No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA400.001789434823.1250021
Produced by FastQC (version 0.11.5)