FastQCFastQC Report
Fri 10 Feb 2017
ERR1632399.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632399.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7156
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC220.3074343208496367No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA170.23756288429290107No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT160.22358859698155395No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA150.20961430967020683No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA150.20961430967020683No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT150.20961430967020683RNA PCR Primer, Index 27 (95% over 23bp)
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA150.20961430967020683No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA120.16769144773616546No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA120.16769144773616546No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA110.15371716042481834No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC110.15371716042481834RNA PCR Primer, Index 27 (95% over 21bp)
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA110.15371716042481834No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA110.15371716042481834No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT100.1397428731134712No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC100.1397428731134712No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC90.1257685858021241No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG90.1257685858021241No Hit
CCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCAC80.11179429849077697No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC80.11179429849077697No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC80.11179429849077697No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC80.11179429849077697No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA80.11179429849077697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT80.11179429849077697No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC80.11179429849077697No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)