FastQCFastQC Report
Fri 10 Feb 2017
ERR1632398.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632398.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69427
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1990.28663200195889205No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1970.28375127832111424No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1730.24918259466778056No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1180.16996269462889077No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1150.16564160917222406No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1080.15555907644000175No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1030.1483572673455572No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1000.14403618188889047No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG930.13395364915666816No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA850.12243075460555693No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA850.12243075460555693No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT820.11810966914889019No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA760.10946749823555676No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGCA200.001830046137.030
TAGGTAC250.005461270529.59999822
GTTAGGT250.005461270529.59999820
ATACTGG250.005461270529.5999986
TACTGTT250.005461270529.59999826
CCCTTGA250.005461270529.5999981
TTGTTAG250.005461270529.59999818
AACGTCA556.120463E-726.9090928
TAACGTC556.120463E-726.9090927
CAAATTC358.78535E-426.4285730
CTTATAC659.12878E-825.61538537
TCTGCGG601.3074168E-624.66666819
CGGGTAA601.3074168E-624.66666823
TGTATCG451.305795E-424.6666685
TGCGGGT601.3074168E-624.66666821
AATGAGC601.3074168E-624.66666834
TAGGACC451.305795E-424.6666684
GTAACGT601.3074168E-624.66666826
TCTTATA701.8693572E-723.78571336
TTAGGAC551.8694867E-523.5454543
Produced by FastQC (version 0.11.5)