##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632398.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	69427
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.05536750831809	34.0	33.0	34.0	31.0	34.0
2	33.19472251429559	34.0	33.0	34.0	31.0	34.0
3	33.27849395768217	34.0	34.0	34.0	31.0	34.0
4	36.53830642257335	37.0	37.0	37.0	35.0	37.0
5	36.51307128350642	37.0	37.0	37.0	35.0	37.0
6	36.57241419044464	37.0	37.0	37.0	35.0	37.0
7	36.565313206677516	37.0	37.0	37.0	35.0	37.0
8	36.54295879124836	37.0	37.0	37.0	35.0	37.0
9	38.324815993777634	39.0	39.0	39.0	37.0	39.0
10	38.35353680844628	39.0	39.0	39.0	37.0	39.0
11	38.42280380831665	39.0	39.0	39.0	37.0	39.0
12	38.38080285767785	39.0	39.0	39.0	37.0	39.0
13	38.41231797427514	39.0	39.0	39.0	37.0	39.0
14	39.99552047474326	41.0	40.0	41.0	38.0	41.0
15	39.99132902185029	41.0	40.0	41.0	38.0	41.0
16	39.970342950149075	41.0	40.0	41.0	38.0	41.0
17	39.970789462312936	41.0	40.0	41.0	38.0	41.0
18	39.96878735938468	41.0	40.0	41.0	38.0	41.0
19	39.97645008426117	41.0	40.0	41.0	38.0	41.0
20	39.95461419908681	41.0	40.0	41.0	38.0	41.0
21	39.91657424344996	41.0	40.0	41.0	38.0	41.0
22	39.8892937906002	41.0	40.0	41.0	38.0	41.0
23	39.84998631656272	41.0	40.0	41.0	38.0	41.0
24	39.833465366500064	41.0	40.0	41.0	38.0	41.0
25	39.784809944258	41.0	40.0	41.0	38.0	41.0
26	39.7032710616907	41.0	40.0	41.0	37.0	41.0
27	39.63486827891166	41.0	40.0	41.0	37.0	41.0
28	39.58814294150691	41.0	40.0	41.0	37.0	41.0
29	39.561352211675576	41.0	40.0	41.0	37.0	41.0
30	39.49724170711683	41.0	40.0	41.0	37.0	41.0
31	39.43334725683091	41.0	40.0	41.0	37.0	41.0
32	39.39117346277385	41.0	39.0	41.0	36.0	41.0
33	39.312371267662435	41.0	39.0	41.0	36.0	41.0
34	39.26269318852896	41.0	39.0	41.0	35.0	41.0
35	39.18652685554611	41.0	39.0	41.0	35.0	41.0
36	39.12624771342561	41.0	39.0	41.0	35.0	41.0
37	39.06686159563282	41.0	39.0	41.0	35.0	41.0
38	38.97512495138779	41.0	39.0	41.0	35.0	41.0
39	38.886009765653135	41.0	39.0	41.0	35.0	41.0
40	38.798867875610355	40.0	39.0	41.0	35.0	41.0
41	38.713670474023075	40.0	39.0	41.0	35.0	41.0
42	38.60665159087963	40.0	38.0	41.0	35.0	41.0
43	37.89216011061979	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	0.0
18	0.0
19	1.0
20	7.0
21	6.0
22	22.0
23	27.0
24	43.0
25	48.0
26	78.0
27	135.0
28	186.0
29	259.0
30	332.0
31	398.0
32	514.0
33	723.0
34	1086.0
35	1581.0
36	2424.0
37	4861.0
38	12388.0
39	44307.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.069310210724936	17.831679317844642	11.911792242211243	26.187218229219177
2	20.13913895170467	20.556843879182452	32.910827199792585	26.39318996932029
3	20.843475881141345	21.34760251775246	29.69593962003255	28.11298198107365
4	14.67728693447794	16.28329036253907	35.43434110648595	33.60508159649704
5	16.011061978769067	34.791939735261494	33.95797024212482	15.239028043844613
6	33.41351347458482	35.237011537298166	15.517017874890172	15.832457113226841
7	29.3776196580581	29.677214916386994	20.83195298659023	20.113212438964666
8	27.090325089662525	31.437337059069236	20.274532962680226	21.197804888588013
9	27.235801633370304	13.42993359932015	19.195701960332435	40.13856280697711
10	18.731905454650207	24.63162746481916	31.566969622769243	25.069497457761393
11	36.77963904532819	20.710962593803565	21.075374133982454	21.434024226885796
12	22.404828092816917	25.207772192374723	28.022239186483645	24.365160528324715
13	32.42974635228369	19.16257363849799	22.841257723940252	25.566422285278062
14	22.933440880349142	20.93853976118801	24.49911417748138	31.628905180981466
15	27.07880219511141	25.851613925418064	22.341452172785804	24.72813170668472
16	24.569691906606938	25.204891468736946	24.418453915623605	25.80696270903251
17	25.00036009045472	25.317239690610283	23.878318233540266	25.804081985394735
18	24.14766589367249	23.787575438950263	25.874659714520288	26.190098952856957
19	25.77527474901695	23.92729053538249	25.971163956385844	24.326270759214715
20	26.741757529491405	23.037146931309145	25.317239690610283	24.903855848589167
21	25.301395710602502	24.21248217552249	25.09542397050139	25.390698143373612
22	26.45080444207585	24.683480490299164	23.897042937185823	24.968672130439167
23	26.1454477364714	23.72708024255693	24.970112492258057	25.157359528713613
24	25.68597231624584	23.856712806256933	24.162069511861382	26.29524536563585
25	25.844412116323625	23.8999236608236	25.354689097901396	24.900975124951387
26	25.897705503622507	24.592737695709165	24.676278681204717	24.833278119463607
27	25.20057038328028	24.16495023549916	25.51889034525473	25.115589035965836
28	24.849122099471387	24.477508750198048	24.719489535771384	25.953879614559177
29	25.286992092413612	25.045011306840276	24.637388912094718	25.03060768865139
30	25.15015771961917	23.958978495398046	25.648522908954728	25.24234087602806
31	25.998530830944734	24.054042375444713	24.785746179440277	25.16168061417028
32	24.166390597318046	23.957538133579153	25.148717357800283	26.727353911302522
33	24.533682861134718	23.77893326803693	25.994209745488067	25.693174125340285
34	24.784305817621384	23.758768202572487	25.704697019891398	25.75222895991473
35	24.48615092111138	25.37197343972806	25.180405317815836	24.961470321344724
36	25.263946303311393	24.074207440909156	25.357569821539172	25.30427643424028
37	25.45119333976695	24.107335762743602	25.30715715787806	25.134313739611393
38	24.80591124490472	24.087170697279156	25.648522908954728	25.458395148861396
39	24.43429789563138	23.837988102611376	26.38598816022585	25.341725841531392
40	24.661875063015827	24.198078557333602	25.998530830944734	25.141515548705833
41	23.64641998069915	24.303224970112492	26.48969421118585	25.56066083800251
42	23.099082489521365	24.794388350353607	26.38454779840696	25.721981361718065
43	23.061633082230255	24.638829273913608	25.9509988909214	26.348538752934736
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12.0
1	9.0
2	6.0
3	13.5
4	21.0
5	21.0
6	26.5
7	32.0
8	31.0
9	30.0
10	42.5
11	55.0
12	55.0
13	106.5
14	158.0
15	233.5
16	309.0
17	297.0
18	285.0
19	285.0
20	354.0
21	423.0
22	358.5
23	294.0
24	350.0
25	406.0
26	406.0
27	466.0
28	526.0
29	641.0
30	756.0
31	976.0
32	1196.0
33	1196.0
34	1455.5
35	1715.0
36	1892.5
37	2070.0
38	2501.0
39	2932.0
40	2932.0
41	3304.0
42	3676.0
43	3982.0
44	4288.0
45	4825.0
46	5362.0
47	5362.0
48	5657.0
49	5952.0
50	6417.5
51	6883.0
52	6801.0
53	6719.0
54	6719.0
55	6237.5
56	5756.0
57	5277.5
58	4799.0
59	4490.5
60	4182.0
61	4182.0
62	3847.0
63	3512.0
64	2901.5
65	2291.0
66	1949.0
67	1607.0
68	1607.0
69	1391.5
70	1176.0
71	957.0
72	738.0
73	600.0
74	462.0
75	462.0
76	384.5
77	307.0
78	254.0
79	201.0
80	167.0
81	133.0
82	133.0
83	105.0
84	77.0
85	61.0
86	45.0
87	33.0
88	21.0
89	21.0
90	16.0
91	11.0
92	6.5
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	69427.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.61700779235744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.02199325512589	61.974447981332915
2	5.495210311795254	7.651201981937862
3	1.8682887468189437	3.901940167370043
4	1.017938054745205	2.834632059573365
5	0.5337967848054124	1.8580667463666873
6	0.3806922806364182	1.590159448053351
7	0.2710363519748412	1.3208117879211259
8	0.19448409989034407	1.0831520878044565
9	0.15517348395506175	0.9722442277500107
>10	0.9993172366705978	13.128897979172368
>50	0.047586535079552274	2.225359010183358
>100	0.014482858502472431	1.4590865225344607
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	199	0.28663200195889205	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	197	0.28375127832111424	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	173	0.24918259466778056	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	118	0.16996269462889077	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	115	0.16564160917222406	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	108	0.15555907644000175	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	103	0.1483572673455572	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	100	0.14403618188889047	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	93	0.13395364915666816	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	85	0.12243075460555693	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	85	0.12243075460555693	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	82	0.11810966914889019	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	76	0.10946749823555676	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0014403618188889048	0.0
10	0.0	0.0	0.0	0.0014403618188889048	0.0
11	0.0	0.0	0.0	0.0014403618188889048	0.0
12	0.0014403618188889048	0.0	0.0	0.0014403618188889048	0.0
13	0.0014403618188889048	0.0	0.0	0.0014403618188889048	0.0
14	0.0014403618188889048	0.0	0.0	0.0014403618188889048	0.0
15	0.0014403618188889048	0.0	0.0	0.0014403618188889048	0.0
16	0.0014403618188889048	0.0	0.0	0.0028807236377778097	0.0
17	0.0014403618188889048	0.0	0.0	0.0028807236377778097	0.0
18	0.0028807236377778097	0.0	0.0	0.0028807236377778097	0.0
19	0.0028807236377778097	0.0	0.0	0.0043210854566667145	0.0
20	0.0028807236377778097	0.0	0.0	0.0043210854566667145	0.0
21	0.0028807236377778097	0.0	0.0	0.005761447275555619	0.0
22	0.0028807236377778097	0.0	0.0	0.008642170913333429	0.0
23	0.0028807236377778097	0.0	0.0	0.008642170913333429	0.0
24	0.0028807236377778097	0.0	0.0	0.011522894551111239	0.0
25	0.0028807236377778097	0.0	0.0	0.014403618188889048	0.0
26	0.0028807236377778097	0.0	0.0	0.018724703645555764	0.0
27	0.0028807236377778097	0.0	0.0	0.031687960015555905	0.0
28	0.0028807236377778097	0.0	0.0	0.07057772912555633	0.0
29	0.0028807236377778097	0.0	0.0	0.12963256370000145	0.0
30	0.0028807236377778097	0.0	0.0	0.22037535829000246	0.0
31	0.0028807236377778097	0.0	0.0	0.4724386765955608	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGCA	20	0.0018300461	37.0	30
TAGGTAC	25	0.0054612705	29.599998	22
GTTAGGT	25	0.0054612705	29.599998	20
ATACTGG	25	0.0054612705	29.599998	6
TACTGTT	25	0.0054612705	29.599998	26
CCCTTGA	25	0.0054612705	29.599998	1
TTGTTAG	25	0.0054612705	29.599998	18
AACGTCA	55	6.120463E-7	26.90909	28
TAACGTC	55	6.120463E-7	26.90909	27
CAAATTC	35	8.78535E-4	26.42857	30
CTTATAC	65	9.12878E-8	25.615385	37
TCTGCGG	60	1.3074168E-6	24.666668	19
CGGGTAA	60	1.3074168E-6	24.666668	23
TGTATCG	45	1.305795E-4	24.666668	5
TGCGGGT	60	1.3074168E-6	24.666668	21
AATGAGC	60	1.3074168E-6	24.666668	34
TAGGACC	45	1.305795E-4	24.666668	4
GTAACGT	60	1.3074168E-6	24.666668	26
TCTTATA	70	1.8693572E-7	23.785713	36
TTAGGAC	55	1.8694867E-5	23.545454	3
>>END_MODULE